Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23370 and PS417_23375 are separated by 11 nucleotides PS417_23375 and PS417_23380 overlap by 1 nucleotides PS417_23380 and PS417_23385 are separated by 3 nucleotides
PS417_23370: PS417_23370 - protoheme IX farnesyltransferase, at 5,059,146 to 5,060,033
_23370
PS417_23375: PS417_23375 - cytochrome C oxidase, at 5,060,045 to 5,060,386
_23375
PS417_23380: PS417_23380 - cytochrome o ubiquinol oxidase subunit III, at 5,060,386 to 5,061,012
_23380
PS417_23385: PS417_23385 - cytochrome o ubiquinol oxidase subunit I, at 5,061,016 to 5,063,034
_23385
Position (kb) 5060
5061
5062 Strain fitness (log2 ratio) -2
-1
0
1 at 5059.565 kb on + strand, within PS417_23370 at 5059.583 kb on - strand, within PS417_23370 at 5059.675 kb on + strand, within PS417_23370 at 5059.676 kb on - strand, within PS417_23370 at 5059.676 kb on - strand, within PS417_23370 at 5059.884 kb on + strand, within PS417_23370 at 5060.065 kb on + strand at 5060.184 kb on + strand, within PS417_23375 at 5060.263 kb on - strand, within PS417_23375 at 5060.516 kb on + strand, within PS417_23380 at 5060.516 kb on + strand, within PS417_23380 at 5060.517 kb on - strand, within PS417_23380 at 5060.730 kb on - strand, within PS417_23380 at 5060.861 kb on + strand, within PS417_23380 at 5060.906 kb on + strand, within PS417_23380 at 5061.002 kb on - strand at 5061.485 kb on + strand, within PS417_23385 at 5061.579 kb on + strand, within PS417_23385 at 5061.620 kb on + strand, within PS417_23385 at 5061.720 kb on - strand, within PS417_23385 at 5061.924 kb on - strand, within PS417_23385 at 5061.924 kb on - strand, within PS417_23385
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 5,059,565 + PS417_23370 0.47 -1.2 5,059,583 - PS417_23370 0.49 -0.6 5,059,675 + PS417_23370 0.60 -2.0 5,059,676 - PS417_23370 0.60 -0.9 5,059,676 - PS417_23370 0.60 -1.3 5,059,884 + PS417_23370 0.83 -1.2 5,060,065 + -0.0 5,060,184 + PS417_23375 0.41 -0.9 5,060,263 - PS417_23375 0.64 -1.3 5,060,516 + PS417_23380 0.21 +0.4 5,060,516 + PS417_23380 0.21 -1.8 5,060,517 - PS417_23380 0.21 -1.3 5,060,730 - PS417_23380 0.55 -2.3 5,060,861 + PS417_23380 0.76 +0.1 5,060,906 + PS417_23380 0.83 -1.3 5,061,002 - -1.4 5,061,485 + PS417_23385 0.23 -1.2 5,061,579 + PS417_23385 0.28 -0.9 5,061,620 + PS417_23385 0.30 -1.3 5,061,720 - PS417_23385 0.35 +1.2 5,061,924 - PS417_23385 0.45 -1.7 5,061,924 - PS417_23385 0.45 -1.4
Or see this region's nucleotide sequence