Strain Fitness in Pseudomonas simiae WCS417 around PS417_02280

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_02270 and PS417_02275 are separated by 0 nucleotidesPS417_02275 and PS417_02280 overlap by 4 nucleotidesPS417_02280 and PS417_02285 are separated by 59 nucleotides PS417_02270: PS417_02270 - carbamoyltransferase, at 518,175 to 519,932 _02270 PS417_02275: PS417_02275 - acetyltransferase, at 519,933 to 520,829 _02275 PS417_02280: PS417_02280 - GlcNAc-PI de-N-acetylase, at 520,826 to 522,226 _02280 PS417_02285: PS417_02285 - glycosyl transferase family 1, at 522,286 to 523,434 _02285 Position (kb) 520 521 522 523Strain fitness (log2 ratio) -1 0 1at 520.642 kb on - strand, within PS417_02275at 520.825 kb on + strandat 520.825 kb on + strandat 521.025 kb on - strand, within PS417_02280at 521.802 kb on + strand, within PS417_02280at 521.839 kb on + strand, within PS417_02280at 522.259 kb on - strandat 522.286 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
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520,642 - PS417_02275 0.79 +1.5
520,825 + -0.2
520,825 + -0.8
521,025 - PS417_02280 0.14 -0.6
521,802 + PS417_02280 0.70 -0.2
521,839 + PS417_02280 0.72 -1.5
522,259 - -0.3
522,286 - +1.5

Or see this region's nucleotide sequence