Strain Fitness in Pseudomonas simiae WCS417 around PS417_21855

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
4,744,451	+				+0.3
4,744,818	-		PS417_21850	0.42	-0.3
4,745,306	+				+0.3
4,745,367	+				-0.3
4,745,367	+				-1.6
4,745,367	+				-0.3
4,745,396	+				+1.4
4,745,403	+				-2.2
4,745,403	+				+0.5
4,745,403	+				-0.1
4,745,404	-				+0.5
4,745,404	-				+1.6
4,745,404	-				-2.8
4,745,448	+				-0.4
4,745,530	+		PS417_21855	0.17	-0.2
4,745,608	-		PS417_21855	0.28	-2.0
4,745,860	-		PS417_21855	0.64	-0.9
4,745,978	-				+1.8
4,746,134	+				-0.4
4,746,134	+				-0.2
4,746,135	-				+0.9
4,746,135	-				+0.2
4,746,138	-				-0.5
4,746,138	-				-0.2
4,746,148	-		PS417_21860	0.13	-0.9
4,746,480	+				-0.2
4,746,480	+		PS417_21865	0.16	-0.2
4,746,481	-		PS417_21865	0.17	+0.0
4,746,481	-		PS417_21865	0.17	-0.1
4,746,482	+		PS417_21865	0.17	-0.1
4,746,482	+		PS417_21865	0.17	-1.1
4,746,668	+		PS417_21865	0.74	-0.4
4,746,669	-		PS417_21865	0.75	-1.7
4,746,753	+				-0.3
4,746,959	+				+0.1
4,746,960	-				-0.1
4,746,982	+				-0.9
4,746,983	-				-0.2