Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_21345 and PS417_21350 are separated by 190 nucleotides PS417_21350 and PS417_21355 are separated by 51 nucleotides PS417_21355 and PS417_21360 are separated by 51 nucleotides PS417_21360 and PS417_21365 are separated by 51 nucleotides
PS417_21345: PS417_21345 - GNAT family acetyltransferase, at 4,639,518 to 4,639,997
_21345
PS417_21350: PS417_21350 - hypothetical protein, at 4,640,188 to 4,640,520
_21350
PS417_21355: PS417_21355 - hypothetical protein, at 4,640,572 to 4,640,838
_21355
PS417_21360: PS417_21360 - termination factor Rho, at 4,640,890 to 4,641,270
_21360
PS417_21365: PS417_21365 - sugar kinase, at 4,641,322 to 4,642,239
_21365
Position (kb)
4640
4641 Strain fitness (log2 ratio)
-1
0
1 at 4639.737 kb on - strand, within PS417_21345 at 4639.738 kb on + strand, within PS417_21345 at 4639.738 kb on + strand, within PS417_21345 at 4639.738 kb on + strand, within PS417_21345 at 4639.784 kb on - strand, within PS417_21345 at 4639.922 kb on - strand, within PS417_21345 at 4640.028 kb on + strand at 4640.028 kb on + strand at 4640.029 kb on - strand at 4640.029 kb on - strand at 4640.029 kb on - strand at 4640.029 kb on - strand at 4640.029 kb on - strand at 4640.029 kb on - strand at 4640.029 kb on - strand at 4640.029 kb on - strand at 4640.029 kb on - strand at 4640.029 kb on - strand at 4640.031 kb on + strand at 4640.031 kb on + strand at 4640.031 kb on + strand at 4640.031 kb on + strand at 4640.151 kb on + strand at 4640.154 kb on + strand at 4640.154 kb on + strand at 4640.155 kb on - strand at 4640.155 kb on - strand at 4640.158 kb on + strand at 4640.159 kb on - strand at 4640.167 kb on + strand at 4640.174 kb on - strand at 4640.567 kb on + strand at 4640.662 kb on + strand, within PS417_21355 at 4640.758 kb on + strand, within PS417_21355 at 4640.836 kb on + strand at 4640.915 kb on - strand at 4641.209 kb on + strand, within PS417_21360 at 4641.459 kb on + strand, within PS417_21365 at 4641.459 kb on + strand, within PS417_21365 at 4641.460 kb on - strand, within PS417_21365 at 4641.464 kb on - strand, within PS417_21365 at 4641.700 kb on + strand, within PS417_21365 at 4641.704 kb on - strand, within PS417_21365 at 4641.831 kb on + strand, within PS417_21365 at 4641.831 kb on + strand, within PS417_21365 at 4641.831 kb on + strand, within PS417_21365
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 4,639,737 - PS417_21345 0.46 +0.4 4,639,738 + PS417_21345 0.46 -0.9 4,639,738 + PS417_21345 0.46 -0.3 4,639,738 + PS417_21345 0.46 +0.1 4,639,784 - PS417_21345 0.55 +0.4 4,639,922 - PS417_21345 0.84 +1.1 4,640,028 + -0.0 4,640,028 + +0.7 4,640,029 - +0.2 4,640,029 - -0.4 4,640,029 - +0.3 4,640,029 - -0.4 4,640,029 - +0.3 4,640,029 - -0.2 4,640,029 - +0.4 4,640,029 - +0.4 4,640,029 - +0.3 4,640,029 - -0.3 4,640,031 + +0.2 4,640,031 + -0.6 4,640,031 + +0.9 4,640,031 + -0.1 4,640,151 + +0.3 4,640,154 + -0.2 4,640,154 + +0.8 4,640,155 - -0.4 4,640,155 - -0.8 4,640,158 + +0.2 4,640,159 - -0.2 4,640,167 + +0.1 4,640,174 - -0.9 4,640,567 + +0.1 4,640,662 + PS417_21355 0.34 -0.8 4,640,758 + PS417_21355 0.70 -0.5 4,640,836 + +0.1 4,640,915 - +1.0 4,641,209 + PS417_21360 0.84 +0.1 4,641,459 + PS417_21365 0.15 -0.5 4,641,459 + PS417_21365 0.15 +0.3 4,641,460 - PS417_21365 0.15 -0.2 4,641,464 - PS417_21365 0.15 -0.0 4,641,700 + PS417_21365 0.41 -1.5 4,641,704 - PS417_21365 0.42 +0.0 4,641,831 + PS417_21365 0.55 +0.2 4,641,831 + PS417_21365 0.55 -0.3 4,641,831 + PS417_21365 0.55 -0.3
Or see this region's nucleotide sequence