Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_20700 and PS417_20705 are separated by 10 nucleotides PS417_20705 and PS417_20710 are separated by 3 nucleotides PS417_20710 and PS417_20715 overlap by 4 nucleotides PS417_20715 and PS417_20720 are separated by 350 nucleotides
PS417_20700: PS417_20700 - cbb3-type cytochrome c oxidase subunit I, at 4,500,217 to 4,501,641
_20700
PS417_20705: PS417_20705 - cbb3-type cytochrome c oxidase subunit II, at 4,501,652 to 4,502,260
_20705
PS417_20710: PS417_20710 - cytochrome C oxidase, at 4,502,264 to 4,502,464
_20710
PS417_20715: PS417_20715 - cytochrome Cbb3, at 4,502,461 to 4,503,408
_20715
PS417_20720: PS417_20720 - cbb3-type cytochrome c oxidase subunit I, at 4,503,759 to 4,505,201
_20720
Position (kb)
4502
4503
4504 Strain fitness (log2 ratio)
-2
-1
0
1 at 4501.477 kb on + strand, within PS417_20700 at 4501.826 kb on + strand, within PS417_20705 at 4501.827 kb on - strand, within PS417_20705 at 4501.827 kb on - strand, within PS417_20705 at 4501.827 kb on - strand, within PS417_20705 at 4501.845 kb on + strand, within PS417_20705 at 4502.148 kb on - strand, within PS417_20705 at 4502.220 kb on - strand at 4502.446 kb on - strand at 4502.478 kb on + strand at 4502.478 kb on + strand at 4502.478 kb on + strand at 4502.478 kb on + strand at 4502.478 kb on + strand at 4502.479 kb on - strand at 4502.492 kb on + strand at 4502.609 kb on - strand, within PS417_20715 at 4502.609 kb on - strand, within PS417_20715 at 4502.609 kb on - strand, within PS417_20715 at 4503.232 kb on + strand, within PS417_20715 at 4503.233 kb on - strand, within PS417_20715 at 4503.238 kb on + strand, within PS417_20715 at 4503.239 kb on - strand, within PS417_20715 at 4503.406 kb on + strand at 4503.511 kb on + strand at 4503.665 kb on + strand at 4503.708 kb on + strand at 4503.708 kb on + strand at 4503.708 kb on + strand at 4503.708 kb on + strand at 4503.786 kb on + strand at 4503.787 kb on - strand at 4503.792 kb on + strand at 4503.792 kb on + strand at 4503.800 kb on - strand at 4503.994 kb on - strand, within PS417_20720 at 4503.997 kb on - strand, within PS417_20720 at 4504.183 kb on - strand, within PS417_20720 at 4504.233 kb on + strand, within PS417_20720 at 4504.234 kb on - strand, within PS417_20720
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 4,501,477 + PS417_20700 0.88 -0.6 4,501,826 + PS417_20705 0.29 -0.3 4,501,827 - PS417_20705 0.29 -0.6 4,501,827 - PS417_20705 0.29 +0.7 4,501,827 - PS417_20705 0.29 +1.7 4,501,845 + PS417_20705 0.32 +0.1 4,502,148 - PS417_20705 0.81 +0.7 4,502,220 - -0.4 4,502,446 - +1.5 4,502,478 + -2.0 4,502,478 + -0.3 4,502,478 + +1.3 4,502,478 + -2.0 4,502,478 + -0.3 4,502,479 - +1.7 4,502,492 + -0.3 4,502,609 - PS417_20715 0.16 -0.3 4,502,609 - PS417_20715 0.16 -0.3 4,502,609 - PS417_20715 0.16 -1.3 4,503,232 + PS417_20715 0.81 +0.7 4,503,233 - PS417_20715 0.81 -2.3 4,503,238 + PS417_20715 0.82 +0.1 4,503,239 - PS417_20715 0.82 -0.9 4,503,406 + -0.9 4,503,511 + +1.4 4,503,665 + +0.3 4,503,708 + -0.2 4,503,708 + -0.4 4,503,708 + -0.6 4,503,708 + -0.9 4,503,786 + +0.7 4,503,787 - -0.1 4,503,792 + +1.1 4,503,792 + +0.2 4,503,800 - +1.1 4,503,994 - PS417_20720 0.16 -0.2 4,503,997 - PS417_20720 0.16 -1.2 4,504,183 - PS417_20720 0.29 +0.6 4,504,233 + PS417_20720 0.33 -1.9 4,504,234 - PS417_20720 0.33 -1.6
Or see this region's nucleotide sequence