Strain Fitness in Pseudomonas simiae WCS417 around PS417_02040

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_02030 and PS417_02035 overlap by 1 nucleotidesPS417_02035 and PS417_02040 overlap by 4 nucleotidesPS417_02040 and PS417_02045 overlap by 4 nucleotides PS417_02030: PS417_02030 - 3-oxoacyl-ACP synthase, at 456,400 to 457,626 _02030 PS417_02035: PS417_02035 - 3-ketoacyl-ACP reductase, at 457,626 to 458,354 _02035 PS417_02040: PS417_02040 - 3-hydroxylacyl-ACP dehydratase, at 458,351 to 458,812 _02040 PS417_02045: PS417_02045 - 3-oxoacyl-ACP synthase, at 458,809 to 459,975 _02045 Position (kb) 458 459Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 457.669 kb on + strandat 457.670 kb on - strandat 457.897 kb on + strand, within PS417_02035at 457.898 kb on - strand, within PS417_02035at 457.953 kb on - strand, within PS417_02035at 458.009 kb on - strand, within PS417_02035at 458.114 kb on - strand, within PS417_02035at 458.378 kb on + strandat 458.379 kb on - strandat 458.408 kb on + strand, within PS417_02040at 458.408 kb on + strand, within PS417_02040at 458.409 kb on - strand, within PS417_02040at 458.409 kb on - strand, within PS417_02040at 458.914 kb on + strandat 458.914 kb on + strandat 458.915 kb on - strandat 459.234 kb on + strand, within PS417_02045at 459.235 kb on - strand, within PS417_02045at 459.599 kb on + strand, within PS417_02045at 459.707 kb on + strand, within PS417_02045at 459.708 kb on - strand, within PS417_02045

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
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457,669 + -0.5
457,670 - +0.1
457,897 + PS417_02035 0.37 -0.0
457,898 - PS417_02035 0.37 +0.2
457,953 - PS417_02035 0.45 -0.6
458,009 - PS417_02035 0.53 +0.1
458,114 - PS417_02035 0.67 -0.2
458,378 + -0.2
458,379 - -1.1
458,408 + PS417_02040 0.12 -0.6
458,408 + PS417_02040 0.12 -0.2
458,409 - PS417_02040 0.13 -0.7
458,409 - PS417_02040 0.13 -0.7
458,914 + -0.2
458,914 + +0.6
458,915 - -0.0
459,234 + PS417_02045 0.36 +0.4
459,235 - PS417_02045 0.37 -4.1
459,599 + PS417_02045 0.68 +0.1
459,707 + PS417_02045 0.77 -0.6
459,708 - PS417_02045 0.77 -0.4

Or see this region's nucleotide sequence