Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01745 and PS417_01750 are separated by 38 nucleotides PS417_01750 and PS417_01755 are separated by 161 nucleotides
PS417_01745: PS417_01745 - histidine ammonia-lyase, at 386,938 to 388,446
_01745
PS417_01750: PS417_01750 - histidine ammonia-lyase, at 388,485 to 390,017
_01750
PS417_01755: PS417_01755 - proline-specific permease, at 390,179 to 391,588
_01755
Position (kb) 388
389
390
391 Strain fitness (log2 ratio) -2
-1
0
1 at 387.821 kb on + strand, within PS417_01745 at 387.821 kb on + strand, within PS417_01745 at 387.822 kb on - strand, within PS417_01745 at 388.096 kb on + strand, within PS417_01745 at 388.096 kb on + strand, within PS417_01745 at 388.097 kb on - strand, within PS417_01745 at 388.363 kb on + strand at 388.364 kb on - strand at 388.478 kb on + strand at 388.479 kb on - strand at 388.538 kb on + strand at 388.539 kb on - strand at 388.542 kb on + strand at 388.879 kb on - strand, within PS417_01750 at 388.977 kb on + strand, within PS417_01750 at 388.978 kb on - strand, within PS417_01750 at 389.204 kb on + strand, within PS417_01750 at 389.393 kb on - strand at 389.910 kb on + strand at 390.058 kb on + strand at 390.059 kb on - strand at 390.062 kb on + strand at 390.062 kb on + strand at 390.063 kb on - strand at 390.063 kb on - strand at 390.063 kb on - strand at 390.063 kb on - strand at 390.092 kb on - strand at 390.092 kb on - strand at 390.092 kb on - strand at 390.168 kb on - strand at 390.168 kb on - strand at 390.226 kb on + strand at 390.226 kb on + strand at 390.226 kb on + strand at 390.227 kb on - strand at 390.227 kb on - strand at 390.272 kb on + strand at 390.273 kb on - strand at 390.425 kb on + strand, within PS417_01755 at 390.425 kb on + strand, within PS417_01755 at 390.425 kb on + strand, within PS417_01755 at 390.464 kb on + strand, within PS417_01755 at 390.514 kb on + strand, within PS417_01755 at 390.518 kb on + strand, within PS417_01755 at 390.518 kb on + strand, within PS417_01755 at 390.519 kb on - strand, within PS417_01755 at 390.519 kb on - strand, within PS417_01755 at 390.519 kb on - strand, within PS417_01755 at 390.519 kb on - strand, within PS417_01755 at 390.927 kb on - strand, within PS417_01755 at 390.927 kb on - strand, within PS417_01755 at 390.927 kb on - strand, within PS417_01755 at 390.955 kb on + strand, within PS417_01755 at 390.956 kb on - strand, within PS417_01755 at 390.960 kb on - strand, within PS417_01755
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 387,821 + PS417_01745 0.59 +0.0 387,821 + PS417_01745 0.59 -0.1 387,822 - PS417_01745 0.59 -0.1 388,096 + PS417_01745 0.77 +0.0 388,096 + PS417_01745 0.77 -0.5 388,097 - PS417_01745 0.77 +0.0 388,363 + -0.5 388,364 - -0.4 388,478 + +0.5 388,479 - -0.0 388,538 + -0.3 388,539 - -2.1 388,542 + +0.1 388,879 - PS417_01750 0.26 -0.3 388,977 + PS417_01750 0.32 -0.4 388,978 - PS417_01750 0.32 -0.1 389,204 + PS417_01750 0.47 -1.8 389,393 - -1.4 389,910 + -0.4 390,058 + -0.3 390,059 - +0.5 390,062 + -1.5 390,062 + -0.2 390,063 - -0.4 390,063 - +0.1 390,063 - -0.4 390,063 - -0.2 390,092 - -2.7 390,092 - +0.9 390,092 - -1.4 390,168 - -2.1 390,168 - -0.2 390,226 + +0.3 390,226 + +0.3 390,226 + -0.3 390,227 - -0.2 390,227 - +0.0 390,272 + -0.0 390,273 - +0.0 390,425 + PS417_01755 0.17 -1.1 390,425 + PS417_01755 0.17 -0.6 390,425 + PS417_01755 0.17 +0.4 390,464 + PS417_01755 0.20 -0.2 390,514 + PS417_01755 0.24 -0.4 390,518 + PS417_01755 0.24 +0.4 390,518 + PS417_01755 0.24 +1.2 390,519 - PS417_01755 0.24 +0.1 390,519 - PS417_01755 0.24 -0.2 390,519 - PS417_01755 0.24 -0.8 390,519 - PS417_01755 0.24 +0.2 390,927 - PS417_01755 0.53 +0.3 390,927 - PS417_01755 0.53 +0.7 390,927 - PS417_01755 0.53 -1.0 390,955 + PS417_01755 0.55 -0.3 390,956 - PS417_01755 0.55 -1.2 390,960 - PS417_01755 0.55 +0.7
Or see this region's nucleotide sequence