Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17480 and PS417_17485 are separated by 30 nucleotides PS417_17485 and PS417_17490 overlap by 4 nucleotides PS417_17490 and PS417_17495 overlap by 4 nucleotides
PS417_17480: PS417_17480 - branched-chain amino acid ABC transporter substrate-binding protein, at 3,818,835 to 3,820,007
_17480
PS417_17485: PS417_17485 - membrane protein, at 3,820,038 to 3,820,997
_17485
PS417_17490: PS417_17490 - ABC transporter ATP-binding protein, at 3,820,994 to 3,821,734
_17490
PS417_17495: PS417_17495 - multidrug ABC transporter permease, at 3,821,731 to 3,822,522
_17495
Position (kb)
3820
3821 Strain fitness (log2 ratio)
-1
0
1 at 3819.068 kb on + strand, within PS417_17480 at 3819.069 kb on - strand, within PS417_17480 at 3819.089 kb on - strand, within PS417_17480 at 3819.279 kb on - strand, within PS417_17480 at 3819.279 kb on - strand, within PS417_17480 at 3819.565 kb on - strand, within PS417_17480 at 3820.013 kb on + strand at 3820.013 kb on + strand at 3820.230 kb on + strand, within PS417_17485 at 3820.357 kb on - strand, within PS417_17485 at 3820.357 kb on - strand, within PS417_17485 at 3820.357 kb on - strand, within PS417_17485 at 3820.357 kb on - strand, within PS417_17485 at 3820.752 kb on + strand, within PS417_17485 at 3820.752 kb on + strand, within PS417_17485 at 3820.824 kb on + strand, within PS417_17485 at 3820.824 kb on + strand, within PS417_17485 at 3820.862 kb on + strand, within PS417_17485 at 3821.150 kb on + strand, within PS417_17490 at 3821.556 kb on + strand, within PS417_17490 at 3821.556 kb on + strand, within PS417_17490 at 3821.741 kb on - strand at 3821.905 kb on + strand, within PS417_17495 at 3821.905 kb on + strand, within PS417_17495 at 3821.905 kb on + strand, within PS417_17495 at 3821.906 kb on - strand, within PS417_17495 at 3821.923 kb on + strand, within PS417_17495 at 3821.992 kb on + strand, within PS417_17495
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 3,819,068 + PS417_17480 0.20 -0.2 3,819,069 - PS417_17480 0.20 -0.6 3,819,089 - PS417_17480 0.22 -0.4 3,819,279 - PS417_17480 0.38 -0.1 3,819,279 - PS417_17480 0.38 -0.5 3,819,565 - PS417_17480 0.62 -0.7 3,820,013 + +1.5 3,820,013 + -0.0 3,820,230 + PS417_17485 0.20 -0.1 3,820,357 - PS417_17485 0.33 -0.7 3,820,357 - PS417_17485 0.33 +0.3 3,820,357 - PS417_17485 0.33 -0.4 3,820,357 - PS417_17485 0.33 -1.2 3,820,752 + PS417_17485 0.74 -0.3 3,820,752 + PS417_17485 0.74 +0.1 3,820,824 + PS417_17485 0.82 -0.2 3,820,824 + PS417_17485 0.82 -0.1 3,820,862 + PS417_17485 0.86 +0.3 3,821,150 + PS417_17490 0.21 +0.3 3,821,556 + PS417_17490 0.76 +0.1 3,821,556 + PS417_17490 0.76 +0.1 3,821,741 - +0.0 3,821,905 + PS417_17495 0.22 -0.1 3,821,905 + PS417_17495 0.22 +0.1 3,821,905 + PS417_17495 0.22 -0.4 3,821,906 - PS417_17495 0.22 -0.1 3,821,923 + PS417_17495 0.24 -0.1 3,821,992 + PS417_17495 0.33 -0.1
Or see this region's nucleotide sequence