Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_15995 and PS417_16000 are separated by 242 nucleotides PS417_16000 and PS417_16005 overlap by 4 nucleotides PS417_16005 and PS417_16010 are separated by 199 nucleotides
PS417_15995: PS417_15995 - peptidase, at 3,505,456 to 3,506,928
_15995
PS417_16000: PS417_16000 - chemotaxis protein CheY, at 3,507,171 to 3,507,911
_16000
PS417_16005: PS417_16005 - ATPase, at 3,507,908 to 3,509,212
_16005
PS417_16010: PS417_16010 - alkanesulfonate monooxygenase, at 3,509,412 to 3,510,494
_16010
Position (kb)
3507
3508
3509
3510 Strain fitness (log2 ratio)
-2
-1
0
1 at 3506.965 kb on + strand at 3506.965 kb on + strand at 3507.068 kb on - strand at 3507.085 kb on + strand at 3507.085 kb on + strand at 3507.086 kb on - strand at 3507.086 kb on - strand at 3507.096 kb on - strand at 3507.149 kb on + strand at 3507.238 kb on - strand at 3507.260 kb on + strand, within PS417_16000 at 3507.261 kb on - strand, within PS417_16000 at 3507.264 kb on - strand, within PS417_16000 at 3507.840 kb on + strand at 3507.841 kb on - strand at 3507.842 kb on + strand at 3507.867 kb on + strand at 3507.867 kb on + strand at 3507.868 kb on - strand at 3508.022 kb on + strand at 3508.380 kb on + strand, within PS417_16005 at 3508.676 kb on - strand, within PS417_16005 at 3508.827 kb on - strand, within PS417_16005 at 3509.002 kb on - strand, within PS417_16005 at 3509.347 kb on + strand at 3509.347 kb on + strand at 3509.799 kb on - strand, within PS417_16010 at 3509.808 kb on + strand, within PS417_16010 at 3509.868 kb on + strand, within PS417_16010 at 3509.868 kb on + strand, within PS417_16010 at 3509.869 kb on - strand, within PS417_16010 at 3509.869 kb on - strand at 3509.871 kb on + strand, within PS417_16010 at 3509.872 kb on - strand, within PS417_16010 at 3509.931 kb on + strand, within PS417_16010 at 3509.988 kb on + strand, within PS417_16010 at 3509.989 kb on - strand, within PS417_16010
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 3,506,965 + +0.1 3,506,965 + -0.7 3,507,068 - -0.8 3,507,085 + -0.4 3,507,085 + -0.1 3,507,086 - +0.1 3,507,086 - +0.2 3,507,096 - +0.1 3,507,149 + +0.8 3,507,238 - -0.7 3,507,260 + PS417_16000 0.12 +0.5 3,507,261 - PS417_16000 0.12 -0.2 3,507,264 - PS417_16000 0.13 -0.3 3,507,840 + +0.2 3,507,841 - -1.5 3,507,842 + +0.5 3,507,867 + -0.6 3,507,867 + +0.2 3,507,868 - -0.0 3,508,022 + -0.2 3,508,380 + PS417_16005 0.36 -1.5 3,508,676 - PS417_16005 0.59 -1.0 3,508,827 - PS417_16005 0.70 -1.4 3,509,002 - PS417_16005 0.84 +0.3 3,509,347 + -1.9 3,509,347 + +0.4 3,509,799 - PS417_16010 0.36 -1.0 3,509,808 + PS417_16010 0.37 +1.1 3,509,868 + PS417_16010 0.42 +0.4 3,509,868 + PS417_16010 0.42 -0.3 3,509,869 - PS417_16010 0.42 -0.1 3,509,869 - +0.6 3,509,871 + PS417_16010 0.42 +0.1 3,509,872 - PS417_16010 0.42 -0.4 3,509,931 + PS417_16010 0.48 +0.7 3,509,988 + PS417_16010 0.53 -0.3 3,509,989 - PS417_16010 0.53 +0.0
Or see this region's nucleotide sequence