Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01560 and PS417_01565 overlap by 1 nucleotides PS417_01565 and PS417_01570 are separated by 0 nucleotides PS417_01570 and PS417_01575 are separated by 39 nucleotides PS417_01575 and PS417_01580 are separated by 9 nucleotides PS417_01580 and PS417_01585 are separated by 66 nucleotides
PS417_01560: PS417_01560 - imidazoleglycerol-phosphate dehydratase, at 346,158 to 346,751
_01560
PS417_01565: PS417_01565 - imidazole glycerol phosphate synthase, at 346,751 to 347,389
_01565
PS417_01570: PS417_01570 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, at 347,390 to 347,647
_01570
PS417_01575: PS417_01575 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) <small>(from data)</small>, at 347,687 to 348,424
_01575
PS417_01580: PS417_01580 - imidazole glycerol phosphate synthase, at 348,434 to 349,204
_01580
PS417_01585: PS417_01585 - amino acid ABC transporter substrate-binding protein, at 349,271 to 350,026
_01585
Position (kb) 347
348
349 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 346.822 kb on - strand, within PS417_01565 at 347.216 kb on + strand, within PS417_01565 at 347.216 kb on + strand, within PS417_01565 at 347.217 kb on - strand, within PS417_01565 at 347.492 kb on - strand, within PS417_01570 at 347.558 kb on + strand, within PS417_01570 at 347.559 kb on - strand, within PS417_01570 at 347.730 kb on + strand at 347.806 kb on - strand, within PS417_01575 at 347.964 kb on - strand, within PS417_01575 at 347.985 kb on - strand, within PS417_01575 at 348.365 kb on + strand at 348.365 kb on + strand at 348.366 kb on - strand at 348.414 kb on - strand at 348.578 kb on - strand, within PS417_01580 at 348.578 kb on - strand, within PS417_01580 at 349.263 kb on + strand at 349.268 kb on + strand at 349.268 kb on + strand at 349.268 kb on + strand at 349.268 kb on + strand at 349.268 kb on + strand at 349.268 kb on + strand at 349.269 kb on - strand at 349.269 kb on - strand at 349.269 kb on - strand at 349.269 kb on - strand at 349.269 kb on - strand at 349.303 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 346,822 - PS417_01565 0.11 -0.6 347,216 + PS417_01565 0.73 -0.9 347,216 + PS417_01565 0.73 -0.1 347,217 - PS417_01565 0.73 -0.8 347,492 - PS417_01570 0.40 -0.4 347,558 + PS417_01570 0.65 +1.0 347,559 - PS417_01570 0.66 -1.1 347,730 + -1.5 347,806 - PS417_01575 0.16 -0.6 347,964 - PS417_01575 0.38 -0.4 347,985 - PS417_01575 0.40 -1.9 348,365 + -0.5 348,365 + +0.1 348,366 - -0.4 348,414 - -1.2 348,578 - PS417_01580 0.19 -0.2 348,578 - PS417_01580 0.19 -2.2 349,263 + +0.3 349,268 + +1.0 349,268 + -0.1 349,268 + +1.1 349,268 + -0.2 349,268 + +0.4 349,268 + -0.7 349,269 - +0.4 349,269 - +0.1 349,269 - +0.1 349,269 - -0.1 349,269 - -3.2 349,303 + +1.1
Or see this region's nucleotide sequence