Strain Fitness in Pseudomonas simiae WCS417 around PS417_01160

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_01155 and PS417_01160 are separated by 214 nucleotidesPS417_01160 and PS417_01165 overlap by 1 nucleotides PS417_01155: PS417_01155 - glutamate--cysteine ligase, at 253,531 to 255,114 _01155 PS417_01160: PS417_01160 - hypothetical protein, at 255,329 to 255,712 _01160 PS417_01165: PS417_01165 - transcription accessory protein, at 255,712 to 258,036 _01165 Position (kb) 255 256Strain fitness (log2 ratio) -1 0 1at 255.168 kb on + strandat 255.206 kb on + strandat 255.330 kb on + strandat 255.332 kb on + strandat 255.332 kb on + strandat 255.470 kb on - strand, within PS417_01160at 255.471 kb on + strand, within PS417_01160at 255.471 kb on + strand, within PS417_01160at 255.472 kb on - strand, within PS417_01160at 255.544 kb on + strand, within PS417_01160at 255.544 kb on + strand, within PS417_01160at 255.544 kb on + strand, within PS417_01160at 255.545 kb on - strand, within PS417_01160at 255.545 kb on - strand, within PS417_01160at 256.207 kb on + strand, within PS417_01165at 256.656 kb on + strand, within PS417_01165

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
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255,168 + +0.2
255,206 + -0.1
255,330 + -0.0
255,332 + -0.6
255,332 + +0.0
255,470 - PS417_01160 0.37 +0.8
255,471 + PS417_01160 0.37 -0.1
255,471 + PS417_01160 0.37 -0.8
255,472 - PS417_01160 0.37 -0.4
255,544 + PS417_01160 0.56 -0.0
255,544 + PS417_01160 0.56 +0.1
255,544 + PS417_01160 0.56 +0.2
255,545 - PS417_01160 0.56 -0.3
255,545 - PS417_01160 0.56 +0.3
256,207 + PS417_01165 0.21 -0.2
256,656 + PS417_01165 0.41 -0.2

Or see this region's nucleotide sequence