Strain Fitness in Pseudomonas simiae WCS417 around PS417_11305

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_11300 and PS417_11305 overlap by 1 nucleotidesPS417_11305 and PS417_11310 are separated by 329 nucleotides PS417_11300: PS417_11300 - general secretion pathway protein GspE, at 2,419,461 to 2,420,879 _11300 PS417_11305: PS417_11305 - general secretion pathway protein GspF, at 2,420,879 to 2,422,090 _11305 PS417_11310: PS417_11310 - alkaline phosphatase, at 2,422,420 to 2,423,601 _11310 Position (kb) 2420 2421 2422 2423Strain fitness (log2 ratio) -3 -2 -1 0 1at 2420.230 kb on - strand, within PS417_11300at 2420.894 kb on + strandat 2421.269 kb on + strand, within PS417_11305at 2421.339 kb on - strand, within PS417_11305at 2421.443 kb on + strand, within PS417_11305at 2421.444 kb on - strand, within PS417_11305at 2421.444 kb on - strand, within PS417_11305at 2421.444 kb on - strand, within PS417_11305at 2421.597 kb on - strand, within PS417_11305at 2422.072 kb on - strandat 2422.089 kb on - strandat 2422.118 kb on + strandat 2422.119 kb on - strandat 2422.305 kb on + strandat 2422.393 kb on + strandat 2422.547 kb on + strand, within PS417_11310at 2422.548 kb on - strand, within PS417_11310at 2422.621 kb on + strand, within PS417_11310at 2422.708 kb on + strand, within PS417_11310at 2422.709 kb on - strand, within PS417_11310

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
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2,420,230 - PS417_11300 0.54 +0.2
2,420,894 + -0.3
2,421,269 + PS417_11305 0.32 -0.1
2,421,339 - PS417_11305 0.38 -0.0
2,421,443 + PS417_11305 0.47 -0.1
2,421,444 - PS417_11305 0.47 -1.1
2,421,444 - PS417_11305 0.47 -1.3
2,421,444 - PS417_11305 0.47 -3.2
2,421,597 - PS417_11305 0.59 -0.4
2,422,072 - -2.5
2,422,089 - -0.4
2,422,118 + -0.0
2,422,119 - -0.2
2,422,305 + +0.3
2,422,393 + +0.9
2,422,547 + PS417_11310 0.11 +0.0
2,422,548 - PS417_11310 0.11 +0.0
2,422,621 + PS417_11310 0.17 -0.9
2,422,708 + PS417_11310 0.24 +0.3
2,422,709 - PS417_11310 0.24 +0.3

Or see this region's nucleotide sequence