Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10110 and PS417_10120 are separated by 262 nucleotides PS417_10120 and PS417_10125 overlap by 4 nucleotides PS417_10125 and PS417_10130 overlap by 4 nucleotides PS417_10130 and PS417_10135 are separated by 72 nucleotides
PS417_10110: PS417_10110 - hypothetical protein, at 2,167,900 to 2,168,403
_10110
PS417_10120: PS417_10120 - hypothetical protein, at 2,168,666 to 2,169,034
_10120
PS417_10125: PS417_10125 - hypothetical protein, at 2,169,031 to 2,169,627
_10125
PS417_10130: PS417_10130 - hypothetical protein, at 2,169,624 to 2,170,040
_10130
PS417_10135: PS417_10135 - phage tail protein, at 2,170,113 to 2,170,769
_10135
Position (kb)
2169
2170 Strain fitness (log2 ratio)
-1
0
1
2 at 2168.061 kb on + strand, within PS417_10110 at 2168.062 kb on - strand, within PS417_10110 at 2168.062 kb on - strand, within PS417_10110 at 2168.062 kb on - strand, within PS417_10110 at 2168.156 kb on - strand, within PS417_10110 at 2168.156 kb on - strand, within PS417_10110 at 2168.401 kb on + strand at 2168.435 kb on - strand at 2168.486 kb on + strand at 2168.490 kb on + strand at 2168.490 kb on + strand at 2168.491 kb on - strand at 2168.579 kb on + strand at 2168.580 kb on - strand at 2168.580 kb on - strand at 2168.580 kb on - strand at 2168.580 kb on - strand at 2168.580 kb on - strand at 2168.760 kb on - strand, within PS417_10120 at 2168.857 kb on + strand, within PS417_10120 at 2168.858 kb on - strand, within PS417_10120 at 2168.858 kb on - strand, within PS417_10120 at 2169.303 kb on + strand, within PS417_10125 at 2169.490 kb on + strand, within PS417_10125 at 2169.491 kb on - strand at 2169.501 kb on + strand, within PS417_10125 at 2169.502 kb on - strand, within PS417_10125 at 2169.502 kb on - strand, within PS417_10125 at 2169.623 kb on + strand at 2169.624 kb on - strand at 2169.624 kb on - strand at 2169.624 kb on - strand at 2169.652 kb on - strand at 2169.748 kb on - strand, within PS417_10130 at 2169.912 kb on + strand, within PS417_10130 at 2169.913 kb on - strand, within PS417_10130 at 2169.996 kb on + strand, within PS417_10130 at 2169.996 kb on + strand, within PS417_10130 at 2169.996 kb on + strand, within PS417_10130 at 2169.997 kb on - strand, within PS417_10130 at 2169.998 kb on + strand, within PS417_10130 at 2169.999 kb on - strand at 2169.999 kb on - strand at 2170.108 kb on + strand at 2170.108 kb on + strand at 2170.109 kb on - strand at 2170.157 kb on + strand at 2170.158 kb on - strand at 2170.161 kb on + strand at 2170.461 kb on + strand, within PS417_10135 at 2170.462 kb on - strand, within PS417_10135
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 2,168,061 + PS417_10110 0.32 -0.1 2,168,062 - PS417_10110 0.32 +0.1 2,168,062 - PS417_10110 0.32 +0.5 2,168,062 - PS417_10110 0.32 +0.4 2,168,156 - PS417_10110 0.51 +0.3 2,168,156 - PS417_10110 0.51 +1.1 2,168,401 + +0.1 2,168,435 - +0.4 2,168,486 + +0.0 2,168,490 + +0.1 2,168,490 + -0.1 2,168,491 - +0.2 2,168,579 + +0.5 2,168,580 - +0.1 2,168,580 - -0.4 2,168,580 - +0.0 2,168,580 - +2.4 2,168,580 - -1.1 2,168,760 - PS417_10120 0.25 +0.3 2,168,857 + PS417_10120 0.52 -0.0 2,168,858 - PS417_10120 0.52 -0.6 2,168,858 - PS417_10120 0.52 -1.6 2,169,303 + PS417_10125 0.46 -1.1 2,169,490 + PS417_10125 0.77 -0.1 2,169,491 - -0.2 2,169,501 + PS417_10125 0.79 -0.7 2,169,502 - PS417_10125 0.79 -0.9 2,169,502 - PS417_10125 0.79 -0.0 2,169,623 + -0.8 2,169,624 - +0.4 2,169,624 - -0.1 2,169,624 - -0.4 2,169,652 - -0.3 2,169,748 - PS417_10130 0.30 -1.0 2,169,912 + PS417_10130 0.69 -0.0 2,169,913 - PS417_10130 0.69 -0.4 2,169,996 + PS417_10130 0.89 +0.5 2,169,996 + PS417_10130 0.89 -0.9 2,169,996 + PS417_10130 0.89 -0.2 2,169,997 - PS417_10130 0.89 +0.5 2,169,998 + PS417_10130 0.90 +1.5 2,169,999 - -0.6 2,169,999 - -0.1 2,170,108 + +0.0 2,170,108 + +0.3 2,170,109 - +0.3 2,170,157 + -0.4 2,170,158 - -0.2 2,170,161 + +0.2 2,170,461 + PS417_10135 0.53 +0.5 2,170,462 - PS417_10135 0.53 +0.1
Or see this region's nucleotide sequence