Strain Fitness in Pseudomonas simiae WCS417 around PS417_10005

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_09995 and PS417_10000 are separated by 70 nucleotidesPS417_10000 and PS417_10005 overlap by 1 nucleotidesPS417_10005 and PS417_10010 overlap by 4 nucleotidesPS417_10010 and PS417_10015 overlap by 4 nucleotides PS417_09995: PS417_09995 - regulatory protein, at 2,151,466 to 2,151,891 _09995 PS417_10000: PS417_10000 - phage antirepressor protein, at 2,151,962 to 2,152,762 _10000 PS417_10005: PS417_10005 - Pyocin large subunit-like protein, at 2,152,762 to 2,153,556 _10005 PS417_10010: PS417_10010 - Replication protein P, at 2,153,553 to 2,154,266 _10010 PS417_10015: PS417_10015 - hypothetical protein, at 2,154,263 to 2,154,583 _10015 Position (kb) 2152 2153 2154Strain fitness (log2 ratio) -3 -2 -1 0 1at 2151.846 kb on - strand, within PS417_09995at 2152.160 kb on + strand, within PS417_10000at 2152.160 kb on + strand, within PS417_10000at 2152.160 kb on + strand, within PS417_10000at 2152.160 kb on + strand, within PS417_10000at 2152.160 kb on + strand, within PS417_10000at 2152.161 kb on - strand, within PS417_10000at 2152.161 kb on - strandat 2152.161 kb on - strand, within PS417_10000at 2152.161 kb on - strand, within PS417_10000at 2152.161 kb on - strand, within PS417_10000at 2152.235 kb on + strandat 2152.237 kb on + strand, within PS417_10000at 2152.237 kb on + strand, within PS417_10000at 2152.238 kb on - strand, within PS417_10000at 2152.238 kb on - strand, within PS417_10000at 2152.500 kb on - strand, within PS417_10000at 2152.524 kb on - strand, within PS417_10000at 2152.581 kb on - strand, within PS417_10000at 2152.581 kb on - strand, within PS417_10000at 2152.581 kb on - strand, within PS417_10000at 2152.581 kb on - strand, within PS417_10000at 2152.638 kb on - strand, within PS417_10000at 2152.702 kb on - strandat 2153.181 kb on + strandat 2153.213 kb on + strand, within PS417_10005at 2153.213 kb on + strand, within PS417_10005at 2153.301 kb on + strand, within PS417_10005at 2153.302 kb on - strand, within PS417_10005at 2153.302 kb on - strand, within PS417_10005at 2153.471 kb on + strandat 2153.471 kb on + strand, within PS417_10005at 2153.472 kb on - strand, within PS417_10005at 2153.472 kb on - strand, within PS417_10005at 2153.934 kb on + strand, within PS417_10010at 2153.934 kb on + strand, within PS417_10010at 2153.935 kb on - strand, within PS417_10010at 2154.039 kb on + strand, within PS417_10010at 2154.040 kb on - strand, within PS417_10010at 2154.204 kb on - strandat 2154.537 kb on + strand, within PS417_10015

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
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2,151,846 - PS417_09995 0.89 +0.2
2,152,160 + PS417_10000 0.25 +0.5
2,152,160 + PS417_10000 0.25 +0.5
2,152,160 + PS417_10000 0.25 +0.0
2,152,160 + PS417_10000 0.25 -0.4
2,152,160 + PS417_10000 0.25 -0.1
2,152,161 - PS417_10000 0.25 +0.4
2,152,161 - -1.8
2,152,161 - PS417_10000 0.25 +0.0
2,152,161 - PS417_10000 0.25 -0.1
2,152,161 - PS417_10000 0.25 -0.4
2,152,235 + -1.2
2,152,237 + PS417_10000 0.34 -0.8
2,152,237 + PS417_10000 0.34 +0.6
2,152,238 - PS417_10000 0.34 +0.1
2,152,238 - PS417_10000 0.34 +0.2
2,152,500 - PS417_10000 0.67 -1.1
2,152,524 - PS417_10000 0.70 -0.1
2,152,581 - PS417_10000 0.77 -0.1
2,152,581 - PS417_10000 0.77 +0.7
2,152,581 - PS417_10000 0.77 -0.2
2,152,581 - PS417_10000 0.77 -2.9
2,152,638 - PS417_10000 0.84 +0.7
2,152,702 - -2.0
2,153,181 + -1.4
2,153,213 + PS417_10005 0.57 -0.2
2,153,213 + PS417_10005 0.57 -1.3
2,153,301 + PS417_10005 0.68 -0.3
2,153,302 - PS417_10005 0.68 -0.3
2,153,302 - PS417_10005 0.68 -0.7
2,153,471 + -1.5
2,153,471 + PS417_10005 0.89 -2.4
2,153,472 - PS417_10005 0.89 -0.3
2,153,472 - PS417_10005 0.89 -0.3
2,153,934 + PS417_10010 0.53 +1.0
2,153,934 + PS417_10010 0.53 +0.4
2,153,935 - PS417_10010 0.54 -0.4
2,154,039 + PS417_10010 0.68 +0.0
2,154,040 - PS417_10010 0.68 +0.4
2,154,204 - -0.1
2,154,537 + PS417_10015 0.85 +0.1

Or see this region's nucleotide sequence