Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08820 and PS417_08825 are separated by 148 nucleotides PS417_08825 and PS417_08830 are separated by 134 nucleotides PS417_08830 and PS417_08835 are separated by 50 nucleotides PS417_08835 and PS417_08840 overlap by 8 nucleotides
PS417_08820: PS417_08820 - transposase, at 1,919,238 to 1,919,696
_08820
PS417_08825: PS417_08825 - TetR family transcriptional regulator, at 1,919,845 to 1,920,450
_08825
PS417_08830: PS417_08830 - membrane protein, at 1,920,585 to 1,921,499
_08830
PS417_08835: PS417_08835 - ABC transporter, at 1,921,550 to 1,922,338
_08835
PS417_08840: PS417_08840 - serine/threonine protein kinase, at 1,922,331 to 1,923,629
_08840
Position (kb)
1920
1921
1922 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1919.632 kb on + strand, within PS417_08820 at 1919.633 kb on - strand, within PS417_08820 at 1919.633 kb on - strand, within PS417_08820 at 1919.636 kb on + strand, within PS417_08820 at 1919.732 kb on + strand at 1919.733 kb on - strand at 1919.733 kb on - strand at 1919.741 kb on + strand at 1919.746 kb on - strand at 1919.837 kb on + strand at 1919.838 kb on - strand at 1919.838 kb on - strand at 1919.841 kb on + strand at 1919.842 kb on - strand at 1919.842 kb on - strand at 1919.842 kb on - strand at 1919.904 kb on - strand at 1920.194 kb on + strand, within PS417_08825 at 1920.311 kb on + strand at 1920.312 kb on - strand, within PS417_08825 at 1920.317 kb on + strand, within PS417_08825 at 1920.318 kb on - strand, within PS417_08825 at 1920.318 kb on - strand, within PS417_08825 at 1920.318 kb on - strand, within PS417_08825 at 1920.548 kb on - strand at 1920.873 kb on + strand, within PS417_08830 at 1921.111 kb on + strand, within PS417_08830 at 1921.111 kb on + strand, within PS417_08830 at 1921.260 kb on + strand, within PS417_08830 at 1921.260 kb on + strand, within PS417_08830 at 1921.508 kb on + strand at 1921.584 kb on + strand at 1921.584 kb on + strand at 1921.584 kb on + strand at 1921.584 kb on + strand at 1921.585 kb on - strand at 1921.590 kb on + strand at 1921.964 kb on - strand, within PS417_08835 at 1922.055 kb on + strand, within PS417_08835 at 1922.055 kb on + strand, within PS417_08835 at 1922.056 kb on - strand, within PS417_08835 at 1922.056 kb on - strand, within PS417_08835 at 1922.103 kb on + strand, within PS417_08835 at 1922.103 kb on + strand, within PS417_08835 at 1922.116 kb on - strand, within PS417_08835 at 1922.149 kb on - strand, within PS417_08835 at 1922.149 kb on - strand, within PS417_08835 at 1922.149 kb on - strand, within PS417_08835 at 1922.149 kb on - strand, within PS417_08835 at 1922.332 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 1,919,632 + PS417_08820 0.86 -0.1 1,919,633 - PS417_08820 0.86 -0.4 1,919,633 - PS417_08820 0.86 -2.2 1,919,636 + PS417_08820 0.87 -0.3 1,919,732 + +0.1 1,919,733 - -0.4 1,919,733 - -0.3 1,919,741 + -0.5 1,919,746 - -0.7 1,919,837 + -0.4 1,919,838 - +0.8 1,919,838 - -0.3 1,919,841 + +0.0 1,919,842 - -1.4 1,919,842 - -0.0 1,919,842 - +0.3 1,919,904 - -0.2 1,920,194 + PS417_08825 0.58 -0.1 1,920,311 + +3.0 1,920,312 - PS417_08825 0.77 +0.0 1,920,317 + PS417_08825 0.78 +0.1 1,920,318 - PS417_08825 0.78 -0.5 1,920,318 - PS417_08825 0.78 -0.3 1,920,318 - PS417_08825 0.78 +1.0 1,920,548 - +0.1 1,920,873 + PS417_08830 0.31 -0.1 1,921,111 + PS417_08830 0.57 -0.3 1,921,111 + PS417_08830 0.57 -1.1 1,921,260 + PS417_08830 0.74 -0.5 1,921,260 + PS417_08830 0.74 -0.6 1,921,508 + -0.7 1,921,584 + -0.3 1,921,584 + -0.3 1,921,584 + +0.1 1,921,584 + -0.1 1,921,585 - -0.4 1,921,590 + +0.3 1,921,964 - PS417_08835 0.52 -0.4 1,922,055 + PS417_08835 0.64 +0.3 1,922,055 + PS417_08835 0.64 -0.4 1,922,056 - PS417_08835 0.64 +0.3 1,922,056 - PS417_08835 0.64 -0.4 1,922,103 + PS417_08835 0.70 -0.3 1,922,103 + PS417_08835 0.70 +0.1 1,922,116 - PS417_08835 0.72 -0.3 1,922,149 - PS417_08835 0.76 -0.1 1,922,149 - PS417_08835 0.76 +0.3 1,922,149 - PS417_08835 0.76 +0.0 1,922,149 - PS417_08835 0.76 +0.3 1,922,332 + +0.8
Or see this region's nucleotide sequence