Strain Fitness in Pseudomonas simiae WCS417 around PS417_00065

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_00060 and PS417_00065 are separated by 81 nucleotidesPS417_00065 and PS417_00070 are separated by 40 nucleotides PS417_00060: PS417_00060 - glycyl-tRNA synthetase, at 14,341 to 15,294 _00060 PS417_00065: PS417_00065 - 3-methyladenine DNA glycosylase, at 15,376 to 15,933 _00065 PS417_00070: PS417_00070 - lipid A biosynthesis lauroyl acyltransferase, at 15,974 to 16,861 _00070 Position (kb) 15 16Strain fitness (log2 ratio) -2 -1 0 1at 15.309 kb on - strandat 15.375 kb on + strandat 15.412 kb on + strandat 15.412 kb on + strandat 15.412 kb on + strandat 15.421 kb on + strandat 15.422 kb on - strandat 15.524 kb on + strand, within PS417_00065at 15.767 kb on + strand, within PS417_00065at 16.856 kb on + strandat 16.924 kb on + strandat 16.924 kb on + strandat 16.925 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
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15,309 - -0.2
15,375 + +0.1
15,412 + -0.2
15,412 + -1.3
15,412 + -0.9
15,421 + -0.5
15,422 - -1.3
15,524 + PS417_00065 0.27 -2.2
15,767 + PS417_00065 0.70 +0.1
16,856 + +0.0
16,924 + -0.9
16,924 + +0.0
16,925 - -0.1

Or see this region's nucleotide sequence