Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05110 and PS417_05115 are separated by 0 nucleotides PS417_05115 and PS417_05120 are separated by 8 nucleotides PS417_05120 and PS417_05125 overlap by 4 nucleotides PS417_05125 and PS417_05130 are separated by 0 nucleotides PS417_05130 and PS417_05135 overlap by 1 nucleotides
PS417_05110: PS417_05110 - peptidase, at 1,134,909 to 1,136,483
_05110
PS417_05115: PS417_05115 - hypothetical protein, at 1,136,484 to 1,136,810
_05115
PS417_05120: PS417_05120 - RNA polymerase subunit sigma-24, at 1,136,819 to 1,137,397
_05120
PS417_05125: PS417_05125 - sugar ABC transporter substrate-binding protein, at 1,137,394 to 1,138,371
_05125
PS417_05130: PS417_05130 - glutaredoxin, at 1,138,372 to 1,138,722
_05130
PS417_05135: PS417_05135 - nucleoid-associated protein, at 1,138,722 to 1,139,726
_05135
Position (kb)
1137
1138
1139 Strain fitness (log2 ratio)
-1
0
1 at 1136.508 kb on + strand at 1136.508 kb on + strand at 1136.561 kb on - strand at 1137.038 kb on + strand, within PS417_05120 at 1137.039 kb on - strand, within PS417_05120 at 1137.039 kb on - strand, within PS417_05120 at 1137.320 kb on - strand, within PS417_05120 at 1137.758 kb on - strand, within PS417_05125 at 1137.868 kb on - strand, within PS417_05125 at 1138.165 kb on + strand, within PS417_05125 at 1138.166 kb on - strand, within PS417_05125 at 1138.210 kb on + strand, within PS417_05125 at 1138.211 kb on - strand, within PS417_05125 at 1138.469 kb on + strand, within PS417_05130 at 1138.470 kb on - strand, within PS417_05130 at 1138.580 kb on + strand, within PS417_05130 at 1138.727 kb on + strand at 1138.728 kb on - strand at 1138.728 kb on - strand at 1138.945 kb on + strand, within PS417_05135 at 1138.946 kb on - strand, within PS417_05135 at 1138.946 kb on - strand, within PS417_05135 at 1138.946 kb on - strand, within PS417_05135 at 1139.032 kb on + strand, within PS417_05135 at 1139.032 kb on + strand, within PS417_05135 at 1139.177 kb on - strand, within PS417_05135 at 1139.211 kb on - strand, within PS417_05135 at 1139.222 kb on - strand, within PS417_05135 at 1139.222 kb on - strand, within PS417_05135 at 1139.222 kb on - strand, within PS417_05135
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 1,136,508 + -0.6 1,136,508 + -0.8 1,136,561 - +1.0 1,137,038 + PS417_05120 0.38 -0.5 1,137,039 - PS417_05120 0.38 -0.1 1,137,039 - PS417_05120 0.38 -0.1 1,137,320 - PS417_05120 0.87 -0.4 1,137,758 - PS417_05125 0.37 -0.6 1,137,868 - PS417_05125 0.48 -1.3 1,138,165 + PS417_05125 0.79 -1.1 1,138,166 - PS417_05125 0.79 +0.1 1,138,210 + PS417_05125 0.83 -0.2 1,138,211 - PS417_05125 0.84 -0.4 1,138,469 + PS417_05130 0.28 +0.4 1,138,470 - PS417_05130 0.28 -0.1 1,138,580 + PS417_05130 0.59 +0.2 1,138,727 + -0.1 1,138,728 - -0.2 1,138,728 - +0.5 1,138,945 + PS417_05135 0.22 +0.5 1,138,946 - PS417_05135 0.22 +0.1 1,138,946 - PS417_05135 0.22 -0.1 1,138,946 - PS417_05135 0.22 +0.6 1,139,032 + PS417_05135 0.31 +0.1 1,139,032 + PS417_05135 0.31 -0.2 1,139,177 - PS417_05135 0.45 -0.3 1,139,211 - PS417_05135 0.49 -0.4 1,139,222 - PS417_05135 0.50 -0.1 1,139,222 - PS417_05135 0.50 +1.0 1,139,222 - PS417_05135 0.50 -0.0
Or see this region's nucleotide sequence