Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00155 and PS417_00160 are separated by 1 nucleotides PS417_00160 and PS417_00165 are separated by 48 nucleotides PS417_00165 and PS417_00170 are separated by 183 nucleotides
PS417_00155: PS417_00155 - 2-dehydro-3-deoxygalactonokinase, at 32,977 to 33,936
_00155
PS417_00160: PS417_00160 - 2-dehydro-3-deoxy-6-phosphogalactonate aldolase, at 33,938 to 34,558
_00160
PS417_00165: PS417_00165 - galactonate dehydratase, at 34,607 to 35,755
_00165
PS417_00170: PS417_00170 - glucarate transporter, at 35,939 to 37,246
_00170
Position (kb)
34
35
36 Strain fitness (log2 ratio)
-1
0
1
2 at 33.713 kb on - strand, within PS417_00155 at 34.080 kb on - strand at 34.426 kb on + strand, within PS417_00160 at 34.427 kb on - strand, within PS417_00160 at 34.427 kb on - strand, within PS417_00160 at 34.427 kb on - strand, within PS417_00160 at 34.492 kb on + strand, within PS417_00160 at 34.529 kb on + strand at 34.530 kb on - strand at 34.530 kb on - strand at 34.591 kb on - strand at 34.591 kb on - strand at 34.591 kb on - strand at 34.592 kb on + strand at 34.602 kb on - strand at 34.758 kb on - strand, within PS417_00165 at 34.955 kb on + strand, within PS417_00165 at 35.213 kb on + strand, within PS417_00165 at 35.213 kb on + strand, within PS417_00165 at 35.213 kb on + strand, within PS417_00165 at 35.214 kb on - strand, within PS417_00165 at 35.214 kb on - strand, within PS417_00165 at 35.357 kb on + strand, within PS417_00165 at 35.438 kb on + strand, within PS417_00165 at 35.516 kb on + strand, within PS417_00165 at 35.517 kb on - strand, within PS417_00165 at 35.517 kb on - strand, within PS417_00165 at 35.517 kb on - strand, within PS417_00165 at 35.582 kb on + strand, within PS417_00165 at 35.582 kb on + strand, within PS417_00165 at 35.609 kb on + strand, within PS417_00165 at 35.609 kb on + strand, within PS417_00165 at 35.672 kb on + strand at 35.673 kb on - strand at 35.673 kb on - strand at 35.673 kb on - strand at 35.916 kb on + strand at 35.922 kb on + strand at 35.973 kb on + strand at 35.983 kb on + strand at 36.144 kb on - strand, within PS417_00170 at 36.201 kb on + strand, within PS417_00170 at 36.201 kb on + strand, within PS417_00170 at 36.202 kb on - strand, within PS417_00170 at 36.202 kb on - strand, within PS417_00170 at 36.202 kb on - strand, within PS417_00170 at 36.202 kb on - strand, within PS417_00170 at 36.203 kb on + strand, within PS417_00170 at 36.204 kb on - strand, within PS417_00170 at 36.204 kb on - strand, within PS417_00170 at 36.204 kb on - strand, within PS417_00170 at 36.386 kb on + strand, within PS417_00170 at 36.386 kb on + strand, within PS417_00170 at 36.387 kb on - strand, within PS417_00170 at 36.683 kb on + strand, within PS417_00170 at 36.683 kb on + strand, within PS417_00170 at 36.684 kb on - strand, within PS417_00170 at 36.684 kb on - strand, within PS417_00170
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1 remove 33,713 - PS417_00155 0.77 +0.1 34,080 - -1.5 34,426 + PS417_00160 0.79 -0.8 34,427 - PS417_00160 0.79 +0.0 34,427 - PS417_00160 0.79 -0.1 34,427 - PS417_00160 0.79 +1.8 34,492 + PS417_00160 0.89 +0.6 34,529 + +0.7 34,530 - -0.1 34,530 - +0.3 34,591 - -0.3 34,591 - -0.7 34,591 - -0.3 34,592 + +0.3 34,602 - -0.2 34,758 - PS417_00165 0.13 -0.6 34,955 + PS417_00165 0.30 -0.1 35,213 + PS417_00165 0.53 -0.5 35,213 + PS417_00165 0.53 -0.7 35,213 + PS417_00165 0.53 -0.8 35,214 - PS417_00165 0.53 -0.4 35,214 - PS417_00165 0.53 -0.4 35,357 + PS417_00165 0.65 -0.1 35,438 + PS417_00165 0.72 +0.3 35,516 + PS417_00165 0.79 -0.0 35,517 - PS417_00165 0.79 +0.5 35,517 - PS417_00165 0.79 +0.5 35,517 - PS417_00165 0.79 +1.5 35,582 + PS417_00165 0.85 -0.4 35,582 + PS417_00165 0.85 -0.5 35,609 + PS417_00165 0.87 -0.0 35,609 + PS417_00165 0.87 +0.0 35,672 + -0.1 35,673 - +0.3 35,673 - +0.0 35,673 - -0.1 35,916 + +0.4 35,922 + -0.4 35,973 + -0.2 35,983 + -0.1 36,144 - PS417_00170 0.16 +0.2 36,201 + PS417_00170 0.20 -0.1 36,201 + PS417_00170 0.20 +0.4 36,202 - PS417_00170 0.20 +0.4 36,202 - PS417_00170 0.20 +0.2 36,202 - PS417_00170 0.20 -0.3 36,202 - PS417_00170 0.20 +0.1 36,203 + PS417_00170 0.20 +0.4 36,204 - PS417_00170 0.20 -0.2 36,204 - PS417_00170 0.20 +0.1 36,204 - PS417_00170 0.20 -0.3 36,386 + PS417_00170 0.34 +0.7 36,386 + PS417_00170 0.34 -0.1 36,387 - PS417_00170 0.34 -1.6 36,683 + PS417_00170 0.57 +0.3 36,683 + PS417_00170 0.57 -0.2 36,684 - PS417_00170 0.57 +0.0 36,684 - PS417_00170 0.57 -0.3
Or see this region's nucleotide sequence