Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13830 and PS417_13835 are separated by 141 nucleotides PS417_13835 and PS417_13840 are separated by 3 nucleotides
PS417_13830: PS417_13830 - hypothetical protein, at 3,038,493 to 3,039,203
_13830
PS417_13835: PS417_13835 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) (from data) , at 3,039,345 to 3,040,448
_13835
PS417_13840: PS417_13840 - isobutyryl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 3,040,452 to 3,041,603
_13840
Position (kb)
3039
3040
3041 Strain fitness (log2 ratio)
-1
0
1 at 3038.359 kb on + strand at 3038.360 kb on - strand at 3038.718 kb on + strand, within PS417_13830 at 3038.719 kb on - strand, within PS417_13830 at 3038.961 kb on + strand, within PS417_13830 at 3039.109 kb on + strand, within PS417_13830 at 3039.110 kb on - strand, within PS417_13830 at 3039.110 kb on - strand, within PS417_13830 at 3039.181 kb on + strand at 3039.225 kb on + strand at 3039.225 kb on + strand at 3039.226 kb on - strand at 3039.260 kb on - strand at 3039.326 kb on + strand at 3039.327 kb on - strand at 3039.538 kb on + strand at 3039.539 kb on - strand, within PS417_13835 at 3039.808 kb on - strand, within PS417_13835 at 3039.831 kb on + strand, within PS417_13835 at 3039.832 kb on - strand, within PS417_13835 at 3039.867 kb on + strand, within PS417_13835 at 3039.867 kb on + strand, within PS417_13835 at 3039.867 kb on + strand, within PS417_13835 at 3039.868 kb on - strand, within PS417_13835 at 3039.868 kb on - strand, within PS417_13835 at 3039.933 kb on + strand, within PS417_13835 at 3039.933 kb on + strand, within PS417_13835 at 3040.235 kb on - strand, within PS417_13835 at 3040.615 kb on + strand, within PS417_13840 at 3040.616 kb on - strand, within PS417_13840 at 3040.642 kb on - strand, within PS417_13840 at 3040.651 kb on - strand, within PS417_13840 at 3040.725 kb on - strand, within PS417_13840
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1 remove 3,038,359 + +0.0 3,038,360 - -0.2 3,038,718 + PS417_13830 0.32 -0.2 3,038,719 - PS417_13830 0.32 +0.9 3,038,961 + PS417_13830 0.66 -0.2 3,039,109 + PS417_13830 0.87 +0.1 3,039,110 - PS417_13830 0.87 +0.3 3,039,110 - PS417_13830 0.87 -0.5 3,039,181 + -0.6 3,039,225 + -0.0 3,039,225 + -0.3 3,039,226 - -0.2 3,039,260 - +0.5 3,039,326 + +0.0 3,039,327 - -0.7 3,039,538 + +0.9 3,039,539 - PS417_13835 0.18 -0.1 3,039,808 - PS417_13835 0.42 +0.2 3,039,831 + PS417_13835 0.44 -1.3 3,039,832 - PS417_13835 0.44 -1.0 3,039,867 + PS417_13835 0.47 -0.0 3,039,867 + PS417_13835 0.47 -0.6 3,039,867 + PS417_13835 0.47 -0.8 3,039,868 - PS417_13835 0.47 -0.9 3,039,868 - PS417_13835 0.47 -0.5 3,039,933 + PS417_13835 0.53 -0.0 3,039,933 + PS417_13835 0.53 -0.1 3,040,235 - PS417_13835 0.81 -0.4 3,040,615 + PS417_13840 0.14 +0.1 3,040,616 - PS417_13840 0.14 -1.2 3,040,642 - PS417_13840 0.16 -0.1 3,040,651 - PS417_13840 0.17 +0.0 3,040,725 - PS417_13840 0.24 +0.5
Or see this region's nucleotide sequence