Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13005 and PS417_13010 are separated by 14 nucleotides PS417_13010 and PS417_13015 overlap by 4 nucleotides PS417_13015 and PS417_13020 are separated by 308 nucleotides
PS417_13005: PS417_13005 - Asp/Glu racemase, at 2,841,188 to 2,841,940
_13005
PS417_13010: PS417_13010 - alpha/beta hydrolase, at 2,841,955 to 2,842,752
_13010
PS417_13015: PS417_13015 - 6-hydroxynicotinate 3-monooxygenase, at 2,842,749 to 2,843,882
_13015
PS417_13020: PS417_13020 - 2,5-dihydroxypyridine 5,6-dioxygenase, at 2,844,191 to 2,845,243
_13020
Position (kb)
2842
2843
2844 Strain fitness (log2 ratio)
-1
0
1 at 2841.794 kb on + strand, within PS417_13005 at 2841.956 kb on + strand at 2842.326 kb on - strand, within PS417_13010 at 2842.469 kb on + strand, within PS417_13010 at 2842.470 kb on - strand, within PS417_13010 at 2842.792 kb on + strand at 2842.798 kb on + strand at 2842.892 kb on - strand, within PS417_13015 at 2843.221 kb on + strand, within PS417_13015 at 2843.222 kb on - strand, within PS417_13015 at 2843.222 kb on - strand, within PS417_13015 at 2843.224 kb on + strand, within PS417_13015 at 2843.224 kb on + strand, within PS417_13015 at 2843.224 kb on + strand, within PS417_13015 at 2843.227 kb on + strand, within PS417_13015 at 2843.228 kb on - strand, within PS417_13015 at 2843.252 kb on - strand, within PS417_13015 at 2843.252 kb on - strand, within PS417_13015 at 2843.252 kb on - strand, within PS417_13015 at 2843.368 kb on + strand, within PS417_13015 at 2843.447 kb on - strand, within PS417_13015 at 2843.590 kb on + strand, within PS417_13015 at 2843.591 kb on - strand, within PS417_13015 at 2843.785 kb on + strand at 2843.785 kb on + strand at 2843.786 kb on - strand at 2843.815 kb on - strand at 2844.704 kb on + strand, within PS417_13020 at 2844.854 kb on + strand, within PS417_13020 at 2844.854 kb on + strand at 2844.855 kb on - strand, within PS417_13020
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1 remove 2,841,794 + PS417_13005 0.80 -0.9 2,841,956 + +0.2 2,842,326 - PS417_13010 0.46 -0.1 2,842,469 + PS417_13010 0.64 -0.6 2,842,470 - PS417_13010 0.65 +0.8 2,842,792 + -0.1 2,842,798 + -0.7 2,842,892 - PS417_13015 0.13 -0.3 2,843,221 + PS417_13015 0.42 -0.2 2,843,222 - PS417_13015 0.42 -0.8 2,843,222 - PS417_13015 0.42 -0.3 2,843,224 + PS417_13015 0.42 -1.0 2,843,224 + PS417_13015 0.42 -0.1 2,843,224 + PS417_13015 0.42 -0.9 2,843,227 + PS417_13015 0.42 -0.3 2,843,228 - PS417_13015 0.42 -0.5 2,843,252 - PS417_13015 0.44 -1.1 2,843,252 - PS417_13015 0.44 +0.7 2,843,252 - PS417_13015 0.44 -0.4 2,843,368 + PS417_13015 0.55 -0.6 2,843,447 - PS417_13015 0.62 -0.1 2,843,590 + PS417_13015 0.74 -1.2 2,843,591 - PS417_13015 0.74 +0.0 2,843,785 + +0.5 2,843,785 + -0.3 2,843,786 - -0.2 2,843,815 - -0.2 2,844,704 + PS417_13020 0.49 +0.3 2,844,854 + PS417_13020 0.63 +0.2 2,844,854 + -1.4 2,844,855 - PS417_13020 0.63 +1.5
Or see this region's nucleotide sequence