Strain Fitness in Pseudomonas putida KT2440 around PP_5258

Experiment: Acanthamoeba sp., muxed culture-2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Acanthamoeba sp., muxed culture-2
6,001,473	+		PP_5257	0.36	-1.3
6,001,473	+		PP_5257	0.36	-1.1
6,001,473	+		PP_5257	0.36	-3.6
6,001,474	-		PP_5257	0.36	-1.2
6,001,474	-		PP_5257	0.36	-4.5
6,001,474	-		PP_5257	0.36	-1.4
6,001,474	-		PP_5257	0.36	-4.1
6,001,474	-		PP_5257	0.36	-1.1
6,001,512	+		PP_5257	0.39	-1.4
6,001,512	+		PP_5257	0.39	-0.5
6,001,513	-		PP_5257	0.39	-2.9
6,001,513	-		PP_5257	0.39	-3.9
6,001,519	+		PP_5257	0.40	-1.9
6,001,519	+		PP_5257	0.40	-1.5
6,001,522	+		PP_5257	0.40	-0.1
6,001,522	+		PP_5257	0.40	+0.2
6,001,522	+		PP_5257	0.40	-2.3
6,001,522	+		PP_5257	0.40	-0.9
6,001,523	-		PP_5257	0.40	-2.7
6,001,523	-		PP_5257	0.40	-1.2
6,001,523	-		PP_5257	0.40	-1.6
6,001,523	-		PP_5257	0.40	-1.5
6,001,523	-		PP_5257	0.40	-2.2
6,001,705	-		PP_5257	0.54	-3.3
6,001,705	-		PP_5257	0.54	+1.0
6,001,729	+		PP_5257	0.56	-1.2
6,001,730	-		PP_5257	0.56	-1.3
6,001,798	+		PP_5257	0.61	-1.4
6,002,177	+				-1.7
6,002,178	-				-2.0
6,002,355	+				-1.6
6,002,387	+				+1.5
6,002,400	-				+0.2
6,002,442	+				+1.1
6,002,442	+				-0.3
6,002,442	+				-0.8
6,002,442	+				-2.7
6,002,442	+				+0.9
6,002,442	+				-0.6
6,002,443	-				-1.0
6,002,443	-				+2.1
6,002,443	-				-0.1
6,002,443	-				+0.0
6,002,443	-				+1.3
6,002,443	-				-0.0
6,002,519	+				-0.0
6,002,519	+				-2.2
6,002,520	-				+0.3
6,002,520	-				-3.6
6,002,520	-				-2.6
6,002,765	+		PP_5258	0.21	-0.5
6,002,765	+		PP_5258	0.21	-1.0
6,002,765	+		PP_5258	0.21	-0.5
6,002,765	+		PP_5258	0.21	-0.6
6,002,765	+		PP_5258	0.21	-1.1
6,002,766	-		PP_5258	0.21	+0.3
6,002,840	+		PP_5258	0.26	+0.1
6,002,840	+		PP_5258	0.26	-2.6
6,002,841	-		PP_5258	0.26	+0.0
6,002,841	-		PP_5258	0.26	-2.4
6,002,843	+		PP_5258	0.27	-1.7
6,002,843	+		PP_5258	0.27	-0.9
6,002,844	-		PP_5258	0.27	-0.9
6,002,980	-				-0.6
6,002,980	-		PP_5258	0.36	-0.3
6,002,980	-		PP_5258	0.36	-0.6
6,003,009	-		PP_5258	0.38	-1.8
6,003,425	-		PP_5258	0.66	-0.6
6,003,579	-		PP_5258	0.76	-0.4
6,003,579	-		PP_5258	0.76	-2.0
6,003,890	+				-1.8
6,003,890	+				-3.5
6,003,891	-				+0.7
6,003,949	+				-0.1
6,003,949	+				-3.4
6,003,949	+				-2.6
6,003,950	-				+0.8
6,003,987	-				-1.0
6,004,002	+				-2.7
6,004,002	+				-1.1
6,004,003	-				+0.1
6,004,003	-				+0.2
6,004,003	-				-0.1
6,004,003	-				+1.1
6,004,003	-				+1.2
6,004,003	-				+0.8
6,004,006	+				-3.2
6,004,007	-				+0.9
6,004,007	-				-0.4
6,004,022	+				-2.2
6,004,022	+				-2.7
6,004,023	-				+1.5
6,004,031	+				-1.1
6,004,032	-				+0.1
6,004,071	+				-1.4
6,004,071	+				-0.9
6,004,072	-				+1.9
6,004,088	+				-3.8
6,004,279	+		PP_5259	0.23	+1.9
6,004,317	+		PP_5259	0.27	-0.5
6,004,317	+		PP_5259	0.27	-1.5
6,004,317	+		PP_5259	0.27	-0.3
6,004,317	+		PP_5259	0.27	-1.2
6,004,317	+		PP_5259	0.27	-0.8
6,004,317	+		PP_5259	0.27	+0.0
6,004,317	+		PP_5259	0.27	-0.1
6,004,317	+		PP_5259	0.27	-0.6
6,004,317	+		PP_5259	0.27	-1.9
6,004,317	+		PP_5259	0.27	+0.9
6,004,317	+		PP_5259	0.27	+0.7
6,004,317	+		PP_5259	0.27	-1.5
6,004,317	+		PP_5259	0.27	-1.8
6,004,318	-		PP_5259	0.28	+0.5
6,004,318	-		PP_5259	0.28	+1.1
6,004,330	+		PP_5259	0.29	-0.6
6,004,330	+		PP_5259	0.29	-1.4
6,004,331	-		PP_5259	0.29	-0.7
6,004,342	+		PP_5259	0.30	-1.6
6,004,343	-		PP_5259	0.30	+1.8
6,004,343	-		PP_5259	0.30	+1.3
6,004,361	+		PP_5259	0.32	+0.5
6,004,361	+		PP_5259	0.32	-0.0
6,004,361	+		PP_5259	0.32	-2.3
6,004,362	-		PP_5259	0.33	+1.2
6,004,362	-		PP_5259	0.33	-1.7
6,004,362	-		PP_5259	0.33	-0.2
6,004,370	+		PP_5259	0.33	-0.7
6,004,370	+		PP_5259	0.33	+0.9
6,004,371	-		PP_5259	0.34	+0.4
6,004,516	+		PP_5259	0.50	-0.3
6,004,517	-		PP_5259	0.50	+0.6
6,004,517	-		PP_5259	0.50	+1.5
6,004,517	-		PP_5259	0.50	+0.2
6,004,720	+		PP_5259	0.73	-0.4
6,004,740	+		PP_5259	0.75	+1.6
6,004,740	+		PP_5259	0.75	-1.9
6,004,741	-		PP_5259	0.75	-0.1
6,004,747	+		PP_5259	0.76	-0.2
6,004,747	+		PP_5259	0.76	+2.5
6,004,747	+		PP_5259	0.76	+1.5
6,004,747	+		PP_5259	0.76	-1.3
6,004,747	+		PP_5259	0.76	+0.2
6,004,747	+		PP_5259	0.76	-0.4
6,004,747	+		PP_5259	0.76	-0.7
6,004,748	-		PP_5259	0.76	-1.5
6,004,748	-		PP_5259	0.76	+2.2
6,004,748	-		PP_5259	0.76	-2.4
6,004,748	-		PP_5259	0.76	+0.3
6,004,748	-		PP_5259	0.76	-0.4
6,004,748	-		PP_5259	0.76	-0.4
6,004,748	-		PP_5259	0.76	+0.5
6,004,748	-		PP_5259	0.76	+0.2
6,004,748	-		PP_5259	0.76	+0.7
6,004,749	+		PP_5259	0.76	-1.2
6,004,749	+		PP_5259	0.76	-0.1
6,004,749	+		PP_5259	0.76	-1.5
6,004,750	-		PP_5259	0.76	-0.2
6,004,801	+		PP_5259	0.82	-0.2
6,004,879	+				+0.3
6,004,880	-				-0.4
6,004,880	-				+0.2
6,004,889	-				+0.2