Strain Fitness in Pseudomonas putida KT2440 around PP_5029

Experiment: Acanthamoeba sp., muxed culture-2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Acanthamoeba sp., muxed culture-2
5,729,576	+		PP_5028	0.17	-1.8
5,729,669	+		PP_5028	0.25	+0.4
5,729,882	+		PP_5028	0.45	+0.3
5,729,882	+		PP_5028	0.45	+2.5
5,729,882	+		PP_5028	0.45	+0.3
5,729,882	+		PP_5028	0.45	-0.5
5,729,882	+		PP_5028	0.45	+1.1
5,729,883	-		PP_5028	0.45	-0.9
5,729,894	+		PP_5028	0.46	-2.2
5,729,894	+		PP_5028	0.46	-2.5
5,729,927	+		PP_5028	0.49	+1.1
5,730,064	+		PP_5028	0.62	-1.6
5,730,064	+		PP_5028	0.62	+0.0
5,730,185	+		PP_5028	0.73	+1.7
5,730,203	+		PP_5028	0.75	+0.7
5,730,203	+		PP_5028	0.75	+0.4
5,730,293	+		PP_5028	0.83	-0.6
5,730,293	+		PP_5028	0.83	-1.7
5,730,294	-		PP_5028	0.83	+2.5
5,730,294	-		PP_5028	0.83	-1.8
5,730,387	+				-0.4
5,730,387	+				-0.9
5,730,387	+				-1.4
5,730,408	-				+1.0
5,730,546	+				-0.6
5,730,546	+				+0.3
5,730,547	-				+1.3
5,730,547	-				+0.2
5,730,581	+				-0.2
5,730,581	+				-1.3
5,730,582	-				-0.2
5,730,593	+				-2.0
5,730,594	-				-3.0
5,730,652	+		PP_5029	0.14	-2.4
5,730,652	+		PP_5029	0.14	+0.4
5,730,652	+		PP_5029	0.14	-0.6
5,730,654	+		PP_5029	0.15	+0.4
5,730,654	+		PP_5029	0.15	-3.5
5,730,654	+		PP_5029	0.15	-2.5
5,730,654	+		PP_5029	0.15	-0.3
5,730,654	+		PP_5029	0.15	-0.4
5,730,654	+		PP_5029	0.15	-4.0
5,730,654	+		PP_5029	0.15	-0.4
5,730,654	+		PP_5029	0.15	+3.1
5,730,654	+		PP_5029	0.15	-2.8
5,730,655	-		PP_5029	0.15	+0.1
5,730,655	-		PP_5029	0.15	-1.5
5,730,655	-		PP_5029	0.15	+0.4
5,730,655	-		PP_5029	0.15	-2.0
5,730,655	-		PP_5029	0.15	+0.6
5,730,655	-		PP_5029	0.15	-2.2
5,730,691	+		PP_5029	0.19	-0.2
5,730,691	+		PP_5029	0.19	+0.4
5,730,691	+		PP_5029	0.19	-0.0
5,730,692	-		PP_5029	0.19	-3.0
5,730,712	-		PP_5029	0.22	+0.1
5,730,748	+		PP_5029	0.26	-3.2
5,730,748	+		PP_5029	0.26	-2.0
5,730,749	-		PP_5029	0.26	-1.7
5,730,762	+		PP_5029	0.28	-2.7
5,730,762	+		PP_5029	0.28	+1.2
5,730,835	+		PP_5029	0.37	-2.1
5,730,835	+		PP_5029	0.37	-0.1
5,730,918	+		PP_5029	0.48	-0.2
5,730,919	-		PP_5029	0.48	-1.4
5,730,958	+		PP_5029	0.52	-0.6
5,730,958	+		PP_5029	0.52	-2.7
5,730,958	+		PP_5029	0.52	-1.9
5,730,959	-		PP_5029	0.53	-1.6
5,730,969	+		PP_5029	0.54	+0.7
5,730,969	+		PP_5029	0.54	-1.4
5,730,994	-		PP_5029	0.57	-1.4
5,731,092	+		PP_5029	0.69	-0.8
5,731,093	-		PP_5029	0.69	+0.1
5,731,143	+		PP_5029	0.75	-2.7
5,731,144	-		PP_5029	0.76	-0.8
5,731,188	+		PP_5029	0.81	-1.3
5,731,188	+		PP_5029	0.81	-3.7
5,731,189	-		PP_5029	0.81	-2.0
5,731,328	+				-1.3
5,731,328	+				-0.4
5,731,329	-				-2.1
5,731,329	-				+0.2
5,731,329	-				-0.9
5,731,329	-				-0.2
5,731,380	+				+0.4
5,731,411	+				-0.8
5,731,412	-				-0.8
5,731,700	-		PP_5030	0.29	-3.2
5,732,005	+		PP_5030	0.54	-0.1
5,732,006	-		PP_5030	0.54	-0.2
5,732,184	+		PP_5030	0.69	-2.9
5,732,185	-		PP_5030	0.69	+1.0
5,732,185	-		PP_5030	0.69	-0.7
5,732,243	+		PP_5030	0.74	+0.9
5,732,293	+		PP_5030	0.78	+1.5
5,732,293	+		PP_5030	0.78	-0.7
5,732,293	+		PP_5030	0.78	+0.9
5,732,294	-		PP_5030	0.78	-0.3
5,732,294	-		PP_5030	0.78	+2.0