Strain Fitness in Pseudomonas putida KT2440 around PP_4404

Experiment: Acanthamoeba sp., muxed culture-2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Acanthamoeba sp., muxed culture-2
4,994,668	+		PP_4403	0.26	-2.7
4,994,799	+		PP_4403	0.36	-0.3
4,994,799	+		PP_4403	0.36	-2.3
4,994,800	-		PP_4403	0.36	-0.9
4,994,800	-		PP_4403	0.36	-2.3
4,994,800	-		PP_4403	0.36	-2.8
4,994,829	-		PP_4403	0.39	-1.6
4,994,898	+		PP_4403	0.44	-1.3
4,994,899	-		PP_4403	0.44	-1.4
4,995,000	+		PP_4403	0.52	-1.9
4,995,000	+		PP_4403	0.52	-2.7
4,995,001	-		PP_4403	0.52	-3.0
4,995,001	-		PP_4403	0.52	-1.8
4,995,153	+		PP_4403	0.64	-1.8
4,995,153	+		PP_4403	0.64	-1.2
4,995,154	-		PP_4403	0.64	-0.2
4,995,314	+		PP_4403	0.77	-3.7
4,995,315	-		PP_4403	0.77	-3.0
4,995,315	-		PP_4403	0.77	-1.1
4,995,564	+				-1.4
4,995,565	+				-1.5
4,995,566	-				-1.2
4,995,623	-				-3.4
4,995,631	+				-2.7
4,995,632	-				-1.4
4,995,665	+				-3.9
4,995,665	+				-1.7
4,995,666	-				-1.0
4,995,666	-				-0.8
4,995,666	-				+0.2
4,995,669	+				-1.3
4,995,669	+				-0.7
4,995,669	+				-2.8
4,995,669	+				-1.3
4,995,669	+				-1.4
4,995,670	-				-2.4
4,995,670	-				-1.9
4,995,670	-				+0.5
4,995,670	-				-0.1
4,995,670	-				-2.7
4,995,670	-				-0.9
4,995,704	-				-2.0
4,995,814	+		PP_4404	0.15	-1.4
4,995,973	-		PP_4404	0.26	+0.1
4,996,063	+		PP_4404	0.33	-0.0
4,996,063	+		PP_4404	0.33	-1.6
4,996,063	+		PP_4404	0.33	-3.5
4,996,064	-				+0.8
4,996,064	-		PP_4404	0.33	-0.5
4,996,154	+		PP_4404	0.39	-1.0
4,996,154	+		PP_4404	0.39	-1.4
4,996,156	+		PP_4404	0.39	+0.5
4,996,182	-		PP_4404	0.41	-0.4
4,996,182	-		PP_4404	0.41	-3.4
4,996,241	+		PP_4404	0.46	-1.7
4,996,241	+		PP_4404	0.46	-1.8
4,996,293	+		PP_4404	0.49	-1.0
4,996,294	-		PP_4404	0.49	-1.5
4,996,294	-		PP_4404	0.49	-2.3
4,996,392	+		PP_4404	0.57	-3.3
4,996,392	+		PP_4404	0.57	-0.1
4,996,392	+		PP_4404	0.57	-1.9
4,996,393	-		PP_4404	0.57	-2.3
4,996,393	-		PP_4404	0.57	-0.6
4,996,851	+		PP_4404	0.90	+1.5
4,996,944	+				-1.2
4,996,984	+				+0.3
4,996,984	+				+0.3
4,996,984	+				+0.9
4,996,984	+				-2.4
4,996,984	+				+0.3
4,996,985	-				-0.4
4,996,985	-				-2.1
4,996,985	-				-0.7
4,996,985	-				-2.9
4,996,985	-				+0.0
4,996,985	-				-0.2
4,997,064	+				+0.8
4,997,064	+				+1.2
4,997,064	+				-0.5
4,997,064	+				-2.7
4,997,065	-				-1.1
4,997,065	-				-0.4
4,997,065	-				-1.0
4,997,065	-				-0.9
4,997,065	-				-2.0
4,997,099	+				-1.3
4,997,099	+				-0.4
4,997,100	-				+0.9
4,997,103	+				+1.0
4,997,103	+				-0.5
4,997,104	-				+1.7
4,997,104	-				+0.1
4,997,104	-				-0.7
4,997,142	+				-1.1
4,997,143	-				-0.2
4,997,211	+		PP_4405	0.11	+0.4
4,997,211	+		PP_4405	0.11	+0.9
4,997,211	+		PP_4405	0.11	+0.2
4,997,211	+		PP_4405	0.11	-0.7
4,997,212	-		PP_4405	0.11	+0.6
4,997,427	+		PP_4405	0.33	-0.6
4,997,758	+		PP_4405	0.66	-0.4
4,997,796	+		PP_4405	0.69	+0.2
4,997,796	+		PP_4405	0.69	-0.7
4,997,796	+		PP_4405	0.69	-0.5
4,997,796	+		PP_4405	0.69	-1.0
4,997,796	+		PP_4405	0.69	-0.0
4,997,796	+		PP_4405	0.69	-0.6
4,997,797	-		PP_4405	0.70	-0.4
4,997,823	+		PP_4405	0.72	+1.1
4,997,823	+		PP_4405	0.72	+0.2
4,997,824	-		PP_4405	0.72	+0.2
4,997,859	+		PP_4405	0.76	-0.1
4,997,859	+		PP_4405	0.76	-0.0
4,997,859	+		PP_4405	0.76	-2.7
4,997,859	+		PP_4405	0.76	-2.2
4,997,859	+		PP_4405	0.76	+1.3
4,997,889	+		PP_4405	0.79	-0.1
4,997,890	-		PP_4405	0.79	+2.4
4,997,920	+		PP_4405	0.82	+1.1
4,997,920	+		PP_4405	0.82	+0.0
4,997,921	-		PP_4405	0.82	+1.5
4,997,952	+		PP_4405	0.85	+1.6
4,997,967	+		PP_4405	0.87	+0.6
4,997,968	-		PP_4405	0.87	+0.6