Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4399 and PP_4400 are separated by 228 nucleotides PP_4400 and PP_4401 are separated by 198 nucleotides
PP_4399: PP_4399 - putative Glutamine synthetase, at 4,989,890 to 4,991,131
_4399
PP_4400: PP_4400 - Bkd operon transcriptional regulator, at 4,991,360 to 4,991,845
_4400
PP_4401: PP_4401 - branched-chain alpha-keto acid dehydrogenase complex, alpha subunit, at 4,992,044 to 4,993,276
_4401
Position (kb)
4991
4992 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4990.424 kb on + strand, within PP_4399 at 4990.425 kb on - strand, within PP_4399 at 4990.425 kb on - strand, within PP_4399 at 4990.683 kb on + strand, within PP_4399 at 4990.684 kb on - strand, within PP_4399 at 4990.684 kb on - strand, within PP_4399 at 4990.684 kb on - strand, within PP_4399 at 4990.770 kb on - strand, within PP_4399 at 4990.770 kb on - strand, within PP_4399 at 4990.770 kb on - strand, within PP_4399 at 4990.866 kb on + strand, within PP_4399 at 4990.867 kb on - strand, within PP_4399 at 4990.874 kb on + strand, within PP_4399 at 4990.875 kb on - strand, within PP_4399 at 4991.035 kb on - strand at 4991.233 kb on + strand at 4991.233 kb on + strand at 4991.233 kb on + strand at 4991.249 kb on + strand at 4991.249 kb on + strand at 4991.249 kb on + strand at 4991.249 kb on + strand at 4991.249 kb on + strand at 4991.249 kb on + strand at 4991.249 kb on + strand at 4991.250 kb on - strand at 4991.250 kb on - strand at 4991.250 kb on - strand at 4991.250 kb on - strand at 4991.250 kb on - strand at 4991.250 kb on - strand at 4991.281 kb on + strand at 4991.281 kb on + strand at 4991.282 kb on - strand at 4991.324 kb on + strand at 4991.410 kb on - strand, within PP_4400 at 4991.410 kb on - strand, within PP_4400 at 4991.410 kb on - strand, within PP_4400 at 4991.444 kb on - strand, within PP_4400 at 4991.444 kb on - strand, within PP_4400 at 4991.473 kb on - strand, within PP_4400 at 4991.550 kb on + strand, within PP_4400 at 4991.551 kb on - strand, within PP_4400 at 4991.551 kb on - strand, within PP_4400 at 4991.551 kb on - strand, within PP_4400 at 4991.638 kb on + strand, within PP_4400 at 4991.639 kb on - strand, within PP_4400 at 4991.845 kb on + strand at 4991.952 kb on + strand at 4991.953 kb on - strand at 4992.054 kb on - strand at 4992.197 kb on + strand, within PP_4401 at 4992.359 kb on + strand, within PP_4401 at 4992.359 kb on + strand, within PP_4401 at 4992.360 kb on - strand, within PP_4401 at 4992.360 kb on - strand, within PP_4401 at 4992.552 kb on - strand, within PP_4401 at 4992.552 kb on - strand, within PP_4401 at 4992.552 kb on - strand, within PP_4401 at 4992.552 kb on - strand, within PP_4401 at 4992.653 kb on - strand, within PP_4401 at 4992.689 kb on - strand, within PP_4401 at 4992.732 kb on + strand, within PP_4401 at 4992.732 kb on + strand, within PP_4401 at 4992.733 kb on - strand, within PP_4401 at 4992.733 kb on - strand, within PP_4401 at 4992.733 kb on - strand, within PP_4401 at 4992.734 kb on + strand, within PP_4401
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 4,990,424 + PP_4399 0.43 -0.2 4,990,425 - PP_4399 0.43 -2.0 4,990,425 - PP_4399 0.43 -0.6 4,990,683 + PP_4399 0.64 +0.9 4,990,684 - PP_4399 0.64 +0.8 4,990,684 - PP_4399 0.64 +0.3 4,990,684 - PP_4399 0.64 -2.3 4,990,770 - PP_4399 0.71 +0.2 4,990,770 - PP_4399 0.71 +0.8 4,990,770 - PP_4399 0.71 -0.8 4,990,866 + PP_4399 0.79 -0.2 4,990,867 - PP_4399 0.79 -0.3 4,990,874 + PP_4399 0.79 -1.4 4,990,875 - PP_4399 0.79 +0.1 4,991,035 - -1.7 4,991,233 + -1.3 4,991,233 + -0.2 4,991,233 + -0.5 4,991,249 + -1.6 4,991,249 + -1.0 4,991,249 + -2.0 4,991,249 + -3.2 4,991,249 + +1.6 4,991,249 + -0.2 4,991,249 + +0.4 4,991,250 - +0.4 4,991,250 - -1.3 4,991,250 - -0.8 4,991,250 - -1.0 4,991,250 - -0.4 4,991,250 - +0.3 4,991,281 + +0.4 4,991,281 + -0.4 4,991,282 - -1.2 4,991,324 + +0.1 4,991,410 - PP_4400 0.10 -2.1 4,991,410 - PP_4400 0.10 -2.4 4,991,410 - PP_4400 0.10 -3.0 4,991,444 - PP_4400 0.17 -1.7 4,991,444 - PP_4400 0.17 -2.2 4,991,473 - PP_4400 0.23 -0.6 4,991,550 + PP_4400 0.39 -2.1 4,991,551 - PP_4400 0.39 -1.2 4,991,551 - PP_4400 0.39 -3.3 4,991,551 - PP_4400 0.39 -4.1 4,991,638 + PP_4400 0.57 +0.2 4,991,639 - PP_4400 0.57 -3.7 4,991,845 + -2.7 4,991,952 + +0.7 4,991,953 - -2.0 4,992,054 - -2.6 4,992,197 + PP_4401 0.12 -2.0 4,992,359 + PP_4401 0.26 -3.3 4,992,359 + PP_4401 0.26 -2.9 4,992,360 - PP_4401 0.26 -1.5 4,992,360 - PP_4401 0.26 -3.5 4,992,552 - PP_4401 0.41 -3.3 4,992,552 - PP_4401 0.41 -4.3 4,992,552 - PP_4401 0.41 -1.9 4,992,552 - PP_4401 0.41 -0.9 4,992,653 - PP_4401 0.49 -3.9 4,992,689 - PP_4401 0.52 -1.7 4,992,732 + PP_4401 0.56 -3.5 4,992,732 + PP_4401 0.56 -3.3 4,992,733 - PP_4401 0.56 -2.5 4,992,733 - PP_4401 0.56 -0.8 4,992,733 - PP_4401 0.56 -3.0 4,992,734 + PP_4401 0.56 -3.5
Or see this region's nucleotide sequence