Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4394 and PP_4395 are separated by 164 nucleotides PP_4395 and PP_4396 are separated by 49 nucleotides PP_4396 and PP_4397 are separated by 79 nucleotides PP_4397 and PP_4398 are separated by 93 nucleotides
PP_4394: PP_4394 - flagella basal body P-ring formation protein, at 4,985,759 to 4,986,511
_4394
PP_4395: PP_4395 - Negative regulator of flagellin synthesis FlgM, at 4,986,676 to 4,986,990
_4395
PP_4396: PP_4396 - conserved protein of unknown function, at 4,987,040 to 4,987,507
_4396
PP_4397: PP_4397 - Flagellar brake protein YcgR, at 4,987,587 to 4,988,330
_4397
PP_4398: PP_4398 - Major facilitator family transporter, at 4,988,424 to 4,989,758
_4398
Position (kb)
4987
4988 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4986.072 kb on + strand, within PP_4394 at 4986.072 kb on + strand, within PP_4394 at 4986.102 kb on + strand, within PP_4394 at 4986.102 kb on + strand, within PP_4394 at 4986.505 kb on + strand at 4986.505 kb on + strand at 4986.505 kb on + strand at 4986.505 kb on + strand at 4986.505 kb on + strand at 4986.505 kb on + strand at 4986.505 kb on + strand at 4986.506 kb on - strand at 4986.506 kb on - strand at 4986.506 kb on - strand at 4986.506 kb on - strand at 4986.506 kb on - strand at 4986.506 kb on - strand at 4986.509 kb on + strand at 4986.509 kb on + strand at 4986.509 kb on + strand at 4986.510 kb on - strand at 4986.510 kb on - strand at 4986.510 kb on - strand at 4986.510 kb on - strand at 4986.510 kb on - strand at 4986.510 kb on - strand at 4986.554 kb on + strand at 4986.555 kb on - strand at 4986.614 kb on - strand at 4986.657 kb on + strand at 4986.994 kb on + strand at 4987.036 kb on - strand at 4987.036 kb on - strand at 4987.275 kb on + strand, within PP_4396 at 4987.275 kb on + strand, within PP_4396 at 4987.276 kb on - strand, within PP_4396 at 4987.448 kb on + strand, within PP_4396 at 4987.453 kb on + strand, within PP_4396 at 4987.515 kb on - strand at 4987.532 kb on - strand at 4987.549 kb on - strand at 4987.581 kb on + strand at 4987.581 kb on + strand at 4987.581 kb on + strand at 4987.581 kb on + strand at 4987.581 kb on + strand at 4987.582 kb on - strand at 4987.582 kb on - strand at 4987.582 kb on - strand at 4987.582 kb on - strand at 4987.728 kb on + strand, within PP_4397 at 4987.728 kb on + strand, within PP_4397 at 4987.728 kb on + strand, within PP_4397 at 4987.728 kb on + strand, within PP_4397 at 4987.729 kb on - strand, within PP_4397 at 4987.729 kb on - strand, within PP_4397 at 4987.729 kb on - strand, within PP_4397 at 4987.729 kb on - strand, within PP_4397 at 4987.729 kb on - strand, within PP_4397 at 4987.729 kb on - strand, within PP_4397 at 4987.849 kb on + strand at 4987.849 kb on + strand, within PP_4397 at 4987.850 kb on - strand, within PP_4397 at 4987.850 kb on - strand, within PP_4397 at 4987.850 kb on - strand at 4987.850 kb on - strand, within PP_4397 at 4987.912 kb on - strand, within PP_4397 at 4987.941 kb on + strand, within PP_4397 at 4987.941 kb on + strand, within PP_4397 at 4987.941 kb on + strand, within PP_4397 at 4987.941 kb on + strand, within PP_4397 at 4987.942 kb on - strand, within PP_4397 at 4987.982 kb on - strand, within PP_4397 at 4988.057 kb on + strand, within PP_4397 at 4988.128 kb on - strand, within PP_4397 at 4988.128 kb on - strand, within PP_4397 at 4988.128 kb on - strand, within PP_4397 at 4988.197 kb on - strand, within PP_4397 at 4988.197 kb on - strand, within PP_4397 at 4988.197 kb on - strand, within PP_4397 at 4988.283 kb on + strand at 4988.325 kb on + strand at 4988.325 kb on + strand at 4988.325 kb on + strand at 4988.326 kb on - strand at 4988.326 kb on - strand at 4988.326 kb on - strand at 4988.343 kb on - strand at 4988.343 kb on - strand at 4988.343 kb on - strand at 4988.426 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 4,986,072 + PP_4394 0.42 -2.6 4,986,072 + PP_4394 0.42 -1.7 4,986,102 + PP_4394 0.46 -3.4 4,986,102 + PP_4394 0.46 -0.3 4,986,505 + -0.5 4,986,505 + -1.6 4,986,505 + -1.6 4,986,505 + -0.1 4,986,505 + -3.1 4,986,505 + -2.0 4,986,505 + +0.7 4,986,506 - +0.0 4,986,506 - +0.7 4,986,506 - -0.4 4,986,506 - -3.0 4,986,506 - -0.5 4,986,506 - -1.1 4,986,509 + -0.1 4,986,509 + -0.5 4,986,509 + -0.9 4,986,510 - +0.1 4,986,510 - -0.5 4,986,510 - -0.6 4,986,510 - -0.1 4,986,510 - +0.2 4,986,510 - -0.2 4,986,554 + +0.7 4,986,555 - -0.2 4,986,614 - -0.8 4,986,657 + +0.1 4,986,994 + +0.2 4,987,036 - -1.3 4,987,036 - -2.0 4,987,275 + PP_4396 0.50 -0.0 4,987,275 + PP_4396 0.50 -0.8 4,987,276 - PP_4396 0.50 -1.3 4,987,448 + PP_4396 0.87 -3.5 4,987,453 + PP_4396 0.88 -0.3 4,987,515 - -0.3 4,987,532 - -0.7 4,987,549 - -2.3 4,987,581 + -1.1 4,987,581 + -0.7 4,987,581 + +1.1 4,987,581 + +0.6 4,987,581 + -0.3 4,987,582 - -0.8 4,987,582 - +0.4 4,987,582 - -1.2 4,987,582 - +0.2 4,987,728 + PP_4397 0.19 -0.4 4,987,728 + PP_4397 0.19 -1.2 4,987,728 + PP_4397 0.19 +0.3 4,987,728 + PP_4397 0.19 -0.3 4,987,729 - PP_4397 0.19 -0.6 4,987,729 - PP_4397 0.19 -1.4 4,987,729 - PP_4397 0.19 -0.0 4,987,729 - PP_4397 0.19 -0.7 4,987,729 - PP_4397 0.19 -0.5 4,987,729 - PP_4397 0.19 -0.2 4,987,849 + +0.1 4,987,849 + PP_4397 0.35 -0.0 4,987,850 - PP_4397 0.35 -0.0 4,987,850 - PP_4397 0.35 +0.5 4,987,850 - -0.4 4,987,850 - PP_4397 0.35 -2.5 4,987,912 - PP_4397 0.44 +0.1 4,987,941 + PP_4397 0.48 +1.4 4,987,941 + PP_4397 0.48 +0.1 4,987,941 + PP_4397 0.48 -0.3 4,987,941 + PP_4397 0.48 +0.9 4,987,942 - PP_4397 0.48 -1.7 4,987,982 - PP_4397 0.53 -1.9 4,988,057 + PP_4397 0.63 -1.2 4,988,128 - PP_4397 0.73 +0.1 4,988,128 - PP_4397 0.73 -0.8 4,988,128 - PP_4397 0.73 -2.1 4,988,197 - PP_4397 0.82 +0.8 4,988,197 - PP_4397 0.82 -0.2 4,988,197 - PP_4397 0.82 +0.1 4,988,283 + +0.2 4,988,325 + -0.7 4,988,325 + -0.3 4,988,325 + -1.1 4,988,326 - -0.3 4,988,326 - -1.6 4,988,326 - -1.2 4,988,343 - +0.4 4,988,343 - -0.7 4,988,343 - +0.3 4,988,426 - -0.6
Or see this region's nucleotide sequence