Strain Fitness in Pseudomonas putida KT2440 around PP_4350

Experiment: Acanthamoeba sp., muxed culture-2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Acanthamoeba sp., muxed culture-2
4,941,712	+				+1.3
4,941,852	+		PP_4349	0.20	+0.7
4,941,853	-		PP_4349	0.20	-2.2
4,941,853	-		PP_4349	0.20	+0.1
4,941,925	+		PP_4349	0.28	-0.3
4,941,927	+		PP_4349	0.28	+1.3
4,941,946	+		PP_4349	0.31	+0.1
4,942,031	+		PP_4349	0.40	+0.0
4,942,033	+		PP_4349	0.41	+0.6
4,942,033	+		PP_4349	0.41	+0.3
4,942,142	-		PP_4349	0.53	-0.7
4,942,291	-		PP_4349	0.70	+0.3
4,942,468	-				-0.6
4,942,544	+				+0.1
4,942,587	+				-0.0
4,942,592	-				+0.5
4,942,592	-				+1.7
4,942,592	-				+0.8
4,942,748	-		PP_4350	0.16	+0.2
4,942,800	-		PP_4350	0.20	+0.3
4,942,848	-		PP_4350	0.24	+0.6
4,942,909	+		PP_4350	0.29	-1.4
4,942,963	+		PP_4350	0.34	-3.2
4,943,116	+		PP_4350	0.47	-2.7
4,943,202	+		PP_4350	0.55	-1.3
4,943,203	-		PP_4350	0.55	-1.8
4,943,499	-		PP_4350	0.80	-2.1
4,943,501	+		PP_4350	0.80	-1.9
4,943,565	+		PP_4350	0.86	-0.8
4,943,565	+		PP_4350	0.86	+0.3
4,943,951	+		PP_5673	0.25	+0.7
4,943,956	-		PP_5673	0.25	+0.7
4,943,975	+		PP_5673	0.27	-0.2
4,944,035	-		PP_5673	0.34	-0.3
4,944,075	-		PP_5673	0.38	+0.8
4,944,164	-		PP_5673	0.48	-0.2
4,944,265	-		PP_5673	0.59	+0.4
4,944,350	+		PP_5673	0.68	+0.2
4,944,399	+		PP_5673	0.74	-0.6
4,944,399	+		PP_5673	0.74	+1.2
4,944,400	-		PP_5673	0.74	+0.0
4,944,472	+		PP_5673	0.82	+0.9
4,944,507	-		PP_5673	0.85	+1.3
4,944,600	+				-1.7
4,944,604	+				+0.3
4,944,703	+				-0.3
4,944,709	-				+0.2