Strain Fitness in Pseudomonas putida KT2440 around PP_4225

Experiment: Acanthamoeba sp., muxed culture-2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Acanthamoeba sp., muxed culture-2
4,796,994	+		PP_4224	0.25	+0.6
4,796,994	+		PP_4224	0.25	+0.8
4,797,031	+		PP_4224	0.28	+0.9
4,797,129	-		PP_4224	0.35	-1.0
4,797,129	-		PP_4224	0.35	+1.3
4,797,129	-		PP_4224	0.35	+1.1
4,797,612	+		PP_4224	0.72	-0.8
4,797,613	-		PP_4224	0.72	-0.8
4,797,613	-		PP_4224	0.72	+0.1
4,797,613	-		PP_4224	0.72	+1.4
4,797,617	-		PP_4224	0.72	+0.8
4,798,127	+		PP_4225	0.22	-0.5
4,798,127	+		PP_4225	0.22	-0.7
4,798,127	+		PP_4225	0.22	-0.7
4,798,251	+		PP_4225	0.40	-0.6
4,798,263	-		PP_4225	0.42	-0.2
4,798,324	-		PP_4225	0.50	+0.1
4,798,370	+		PP_4225	0.57	-1.2
4,798,370	+		PP_4225	0.57	-0.4
4,798,691	+				-0.6
4,798,691	+				+0.3
4,798,711	-				-1.1
4,798,721	+				+1.1
4,798,721	+				-1.3
4,798,721	+				-0.1
4,798,845	+				+0.7
4,799,014	-		PP_4226	0.23	-2.1
4,799,083	+		PP_4226	0.37	+0.1
4,799,084	-		PP_4226	0.37	+0.6
4,799,084	-		PP_4226	0.37	+0.8
4,799,084	-		PP_4226	0.37	+1.7
4,799,115	-		PP_4226	0.44	+1.9
4,799,425	+				+1.4
4,799,426	-				+0.6
4,799,426	-				-1.0
4,799,471	+		PP_5664	0.11	+1.8
4,799,471	+		PP_5664	0.11	+1.1
4,799,472	-		PP_5664	0.12	-0.2
4,799,472	-		PP_5664	0.12	+0.9
4,799,476	+		PP_5664	0.12	+1.8
4,799,477	-		PP_5664	0.12	-2.6
4,799,477	-		PP_5664	0.12	+0.5
4,799,517	+		PP_5664	0.19	+0.4
4,799,557	+		PP_5664	0.25	-0.8
4,799,636	+		PP_5664	0.38	-0.2
4,799,636	+		PP_5664	0.38	-0.7
4,799,641	-		PP_5664	0.39	-0.3