Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3723 and PP_3724 overlap by 8 nucleotides PP_3724 and PP_3725 are separated by 17 nucleotides
PP_3723: PP_3723 - putative 2-ketoarginine decarboxylase AruI, at 4,246,309 to 4,247,916
_3723
PP_3724: PP_3724 - putative Acyl-CoA synthetase, at 4,247,909 to 4,250,026
_3724
PP_3725: PP_3725 - putative Acyl-CoA dehydrogenase, at 4,250,044 to 4,251,204
_3725
Position (kb)
4247
4248
4249
4250
4251 Strain fitness (log2 ratio)
-1
0
1
2
3 at 4247.289 kb on + strand, within PP_3723 at 4247.290 kb on - strand, within PP_3723 at 4247.369 kb on - strand, within PP_3723 at 4247.369 kb on - strand, within PP_3723 at 4247.776 kb on + strand at 4247.776 kb on + strand at 4247.777 kb on - strand at 4247.777 kb on - strand at 4247.777 kb on - strand at 4247.777 kb on - strand at 4247.777 kb on - strand at 4247.777 kb on - strand at 4247.777 kb on - strand at 4247.777 kb on - strand at 4247.777 kb on - strand at 4247.777 kb on - strand at 4247.810 kb on + strand at 4247.811 kb on - strand at 4247.862 kb on - strand at 4247.862 kb on - strand at 4247.862 kb on - strand at 4247.862 kb on - strand at 4247.969 kb on - strand at 4248.037 kb on - strand at 4248.037 kb on - strand at 4248.266 kb on + strand, within PP_3724 at 4248.267 kb on - strand, within PP_3724 at 4248.267 kb on - strand, within PP_3724 at 4248.336 kb on + strand, within PP_3724 at 4248.336 kb on + strand, within PP_3724 at 4248.337 kb on - strand, within PP_3724 at 4248.337 kb on - strand, within PP_3724 at 4248.459 kb on - strand, within PP_3724 at 4248.509 kb on - strand, within PP_3724 at 4248.667 kb on - strand, within PP_3724 at 4248.826 kb on - strand, within PP_3724 at 4249.003 kb on - strand, within PP_3724 at 4249.237 kb on - strand, within PP_3724 at 4249.488 kb on + strand, within PP_3724 at 4249.488 kb on + strand, within PP_3724 at 4249.528 kb on - strand, within PP_3724 at 4249.528 kb on - strand, within PP_3724 at 4249.735 kb on - strand, within PP_3724 at 4249.735 kb on - strand, within PP_3724 at 4249.735 kb on - strand, within PP_3724 at 4249.735 kb on - strand, within PP_3724 at 4250.083 kb on - strand at 4250.083 kb on - strand at 4250.364 kb on - strand, within PP_3725 at 4250.567 kb on + strand, within PP_3725 at 4250.567 kb on + strand, within PP_3725 at 4250.567 kb on + strand, within PP_3725 at 4250.576 kb on + strand, within PP_3725 at 4250.577 kb on - strand at 4250.684 kb on + strand, within PP_3725 at 4250.684 kb on + strand, within PP_3725 at 4250.684 kb on + strand, within PP_3725 at 4250.685 kb on - strand, within PP_3725 at 4250.685 kb on - strand, within PP_3725 at 4250.685 kb on - strand, within PP_3725 at 4250.685 kb on - strand, within PP_3725 at 4250.685 kb on - strand at 4250.879 kb on + strand, within PP_3725 at 4250.879 kb on + strand, within PP_3725 at 4250.996 kb on + strand, within PP_3725 at 4250.996 kb on + strand, within PP_3725 at 4250.997 kb on - strand, within PP_3725
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 4,247,289 + PP_3723 0.61 +0.7 4,247,290 - PP_3723 0.61 -0.2 4,247,369 - PP_3723 0.66 -0.7 4,247,369 - PP_3723 0.66 +1.6 4,247,776 + -1.3 4,247,776 + +0.8 4,247,777 - +1.0 4,247,777 - +1.6 4,247,777 - +0.4 4,247,777 - +1.5 4,247,777 - +1.2 4,247,777 - -0.9 4,247,777 - +0.3 4,247,777 - -0.6 4,247,777 - -1.0 4,247,777 - +0.3 4,247,810 + +0.7 4,247,811 - +1.6 4,247,862 - +0.4 4,247,862 - +1.2 4,247,862 - +1.1 4,247,862 - +2.5 4,247,969 - +3.0 4,248,037 - +2.9 4,248,037 - +2.5 4,248,266 + PP_3724 0.17 +0.6 4,248,267 - PP_3724 0.17 +2.1 4,248,267 - PP_3724 0.17 +2.2 4,248,336 + PP_3724 0.20 +0.2 4,248,336 + PP_3724 0.20 +1.6 4,248,337 - PP_3724 0.20 +1.6 4,248,337 - PP_3724 0.20 +1.6 4,248,459 - PP_3724 0.26 +0.5 4,248,509 - PP_3724 0.28 +0.1 4,248,667 - PP_3724 0.36 -0.9 4,248,826 - PP_3724 0.43 -0.3 4,249,003 - PP_3724 0.52 +0.5 4,249,237 - PP_3724 0.63 -0.2 4,249,488 + PP_3724 0.75 -1.3 4,249,488 + PP_3724 0.75 -0.8 4,249,528 - PP_3724 0.76 -1.5 4,249,528 - PP_3724 0.76 +0.6 4,249,735 - PP_3724 0.86 +0.5 4,249,735 - PP_3724 0.86 +0.5 4,249,735 - PP_3724 0.86 +0.1 4,249,735 - PP_3724 0.86 -0.0 4,250,083 - +2.6 4,250,083 - +2.8 4,250,364 - PP_3725 0.28 +1.4 4,250,567 + PP_3725 0.45 -0.7 4,250,567 + PP_3725 0.45 +1.5 4,250,567 + PP_3725 0.45 -1.5 4,250,576 + PP_3725 0.46 -1.0 4,250,577 - +2.0 4,250,684 + PP_3725 0.55 -0.8 4,250,684 + PP_3725 0.55 +0.3 4,250,684 + PP_3725 0.55 -0.0 4,250,685 - PP_3725 0.55 +0.2 4,250,685 - PP_3725 0.55 +0.1 4,250,685 - PP_3725 0.55 +1.0 4,250,685 - PP_3725 0.55 +0.8 4,250,685 - +1.4 4,250,879 + PP_3725 0.72 +0.4 4,250,879 + PP_3725 0.72 +1.0 4,250,996 + PP_3725 0.82 +0.9 4,250,996 + PP_3725 0.82 -0.9 4,250,997 - PP_3725 0.82 +1.9
Or see this region's nucleotide sequence