Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1841 and PP_1842 are separated by 529 nucleotides PP_1842 and PP_1843 are separated by 40 nucleotides
PP_1841: PP_1841 - Cytochrome c family protein, at 2,064,197 to 2,064,997
_1841
PP_1842: PP_1842 - Glutamine amidotransferase, class I, at 2,065,527 to 2,066,261
_1842
PP_1843: PP_1843 - Mg2+/Co2+ transporter, at 2,066,302 to 2,067,273
_1843
Position (kb)
2065
2066
2067 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2064.616 kb on - strand, within PP_1841 at 2064.838 kb on - strand, within PP_1841 at 2064.843 kb on - strand, within PP_1841 at 2064.843 kb on - strand, within PP_1841 at 2064.843 kb on - strand, within PP_1841 at 2064.843 kb on - strand, within PP_1841 at 2064.857 kb on - strand, within PP_1841 at 2064.860 kb on + strand, within PP_1841 at 2064.997 kb on + strand at 2065.029 kb on + strand at 2065.100 kb on + strand at 2065.100 kb on + strand at 2065.100 kb on + strand at 2065.101 kb on - strand at 2065.101 kb on - strand at 2065.101 kb on - strand at 2065.101 kb on - strand at 2065.118 kb on + strand at 2065.181 kb on + strand at 2065.276 kb on + strand at 2065.276 kb on + strand at 2065.276 kb on + strand at 2065.276 kb on + strand at 2065.277 kb on - strand at 2065.277 kb on - strand at 2065.277 kb on - strand at 2065.278 kb on + strand at 2065.278 kb on + strand at 2065.278 kb on + strand at 2065.278 kb on + strand at 2065.278 kb on + strand at 2065.278 kb on + strand at 2065.278 kb on + strand at 2065.279 kb on - strand at 2065.279 kb on - strand at 2065.279 kb on - strand at 2065.290 kb on + strand at 2065.290 kb on + strand at 2065.290 kb on + strand at 2065.290 kb on + strand at 2065.291 kb on - strand at 2065.291 kb on - strand at 2065.291 kb on - strand at 2065.309 kb on - strand at 2065.309 kb on - strand at 2065.372 kb on + strand at 2065.373 kb on - strand at 2065.373 kb on - strand at 2065.377 kb on - strand at 2065.377 kb on - strand at 2065.390 kb on + strand at 2065.394 kb on + strand at 2065.395 kb on - strand at 2065.395 kb on - strand at 2065.395 kb on - strand at 2065.455 kb on + strand at 2065.456 kb on - strand at 2065.456 kb on - strand at 2065.456 kb on - strand at 2065.465 kb on + strand at 2065.465 kb on + strand at 2065.466 kb on - strand at 2065.466 kb on - strand at 2065.488 kb on + strand at 2065.489 kb on - strand at 2065.520 kb on + strand at 2065.520 kb on + strand at 2065.520 kb on + strand at 2065.526 kb on + strand at 2065.526 kb on + strand at 2065.527 kb on - strand at 2065.527 kb on - strand at 2065.527 kb on - strand at 2065.587 kb on + strand at 2065.657 kb on - strand, within PP_1842 at 2065.657 kb on - strand, within PP_1842 at 2065.707 kb on + strand, within PP_1842 at 2065.707 kb on + strand, within PP_1842 at 2065.708 kb on - strand, within PP_1842 at 2065.765 kb on + strand, within PP_1842 at 2065.788 kb on + strand, within PP_1842 at 2065.788 kb on + strand, within PP_1842 at 2065.788 kb on + strand, within PP_1842 at 2066.100 kb on + strand, within PP_1842 at 2066.100 kb on + strand, within PP_1842 at 2066.100 kb on + strand, within PP_1842 at 2066.133 kb on + strand at 2066.133 kb on + strand, within PP_1842 at 2066.133 kb on + strand, within PP_1842 at 2066.133 kb on + strand, within PP_1842 at 2066.134 kb on - strand, within PP_1842 at 2066.222 kb on + strand at 2066.259 kb on + strand at 2066.333 kb on + strand at 2066.333 kb on + strand at 2066.334 kb on - strand at 2066.334 kb on - strand at 2066.334 kb on - strand at 2066.334 kb on - strand at 2066.378 kb on + strand at 2066.378 kb on + strand at 2066.384 kb on + strand at 2066.426 kb on + strand, within PP_1843 at 2066.610 kb on - strand, within PP_1843 at 2066.687 kb on + strand, within PP_1843 at 2066.764 kb on + strand, within PP_1843 at 2066.764 kb on + strand, within PP_1843 at 2066.764 kb on + strand, within PP_1843 at 2066.765 kb on - strand at 2066.765 kb on - strand, within PP_1843 at 2066.819 kb on + strand, within PP_1843 at 2066.820 kb on - strand, within PP_1843 at 2066.849 kb on + strand, within PP_1843 at 2066.954 kb on + strand, within PP_1843 at 2066.955 kb on - strand, within PP_1843 at 2066.973 kb on + strand, within PP_1843 at 2066.973 kb on + strand, within PP_1843 at 2066.974 kb on - strand, within PP_1843 at 2066.974 kb on - strand, within PP_1843 at 2066.974 kb on - strand, within PP_1843 at 2067.020 kb on + strand, within PP_1843 at 2067.020 kb on - strand, within PP_1843 at 2067.245 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 2,064,616 - PP_1841 0.52 +0.8 2,064,838 - PP_1841 0.80 +1.9 2,064,843 - PP_1841 0.81 +1.1 2,064,843 - PP_1841 0.81 +0.8 2,064,843 - PP_1841 0.81 +1.2 2,064,843 - PP_1841 0.81 -0.7 2,064,857 - PP_1841 0.82 -0.2 2,064,860 + PP_1841 0.83 +0.0 2,064,997 + +1.8 2,065,029 + +0.2 2,065,100 + -0.3 2,065,100 + +0.5 2,065,100 + +0.8 2,065,101 - -0.1 2,065,101 - +0.1 2,065,101 - +1.1 2,065,101 - +1.3 2,065,118 + -1.8 2,065,181 + +0.7 2,065,276 + -0.4 2,065,276 + -1.4 2,065,276 + -0.6 2,065,276 + +0.6 2,065,277 - +0.3 2,065,277 - +1.9 2,065,277 - +0.2 2,065,278 + -1.2 2,065,278 + -0.4 2,065,278 + +1.0 2,065,278 + -3.4 2,065,278 + +0.8 2,065,278 + +1.0 2,065,278 + -0.2 2,065,279 - -2.0 2,065,279 - +0.4 2,065,279 - -1.4 2,065,290 + -0.4 2,065,290 + -0.8 2,065,290 + +0.5 2,065,290 + -1.7 2,065,291 - -1.0 2,065,291 - +0.7 2,065,291 - -0.3 2,065,309 - -0.6 2,065,309 - -1.0 2,065,372 + -0.5 2,065,373 - +0.8 2,065,373 - +0.8 2,065,377 - -0.4 2,065,377 - -0.7 2,065,390 + +0.7 2,065,394 + -0.6 2,065,395 - -1.5 2,065,395 - +1.6 2,065,395 - +1.4 2,065,455 + -0.3 2,065,456 - +2.3 2,065,456 - +1.3 2,065,456 - +1.7 2,065,465 + +0.9 2,065,465 + +1.4 2,065,466 - +0.1 2,065,466 - +2.0 2,065,488 + -0.4 2,065,489 - +0.6 2,065,520 + +1.0 2,065,520 + +1.7 2,065,520 + +1.9 2,065,526 + +2.4 2,065,526 + +1.0 2,065,527 - +0.3 2,065,527 - +1.1 2,065,527 - +1.0 2,065,587 + +1.0 2,065,657 - PP_1842 0.18 +1.1 2,065,657 - PP_1842 0.18 +2.3 2,065,707 + PP_1842 0.24 +1.0 2,065,707 + PP_1842 0.24 +1.2 2,065,708 - PP_1842 0.25 +0.7 2,065,765 + PP_1842 0.32 +1.7 2,065,788 + PP_1842 0.36 +0.9 2,065,788 + PP_1842 0.36 +1.0 2,065,788 + PP_1842 0.36 -2.3 2,066,100 + PP_1842 0.78 +2.1 2,066,100 + PP_1842 0.78 +1.2 2,066,100 + PP_1842 0.78 -0.0 2,066,133 + -1.2 2,066,133 + PP_1842 0.82 +1.7 2,066,133 + PP_1842 0.82 +1.9 2,066,133 + PP_1842 0.82 +1.3 2,066,134 - PP_1842 0.83 +1.8 2,066,222 + +1.8 2,066,259 + -0.0 2,066,333 + -2.4 2,066,333 + +0.5 2,066,334 - -0.8 2,066,334 - -0.2 2,066,334 - -0.9 2,066,334 - -1.2 2,066,378 + +0.5 2,066,378 + -2.0 2,066,384 + -2.7 2,066,426 + PP_1843 0.13 +0.5 2,066,610 - PP_1843 0.32 -0.2 2,066,687 + PP_1843 0.40 +0.2 2,066,764 + PP_1843 0.48 +0.2 2,066,764 + PP_1843 0.48 +0.7 2,066,764 + PP_1843 0.48 +0.1 2,066,765 - +0.1 2,066,765 - PP_1843 0.48 -0.7 2,066,819 + PP_1843 0.53 -0.4 2,066,820 - PP_1843 0.53 -0.0 2,066,849 + PP_1843 0.56 -0.7 2,066,954 + PP_1843 0.67 -0.6 2,066,955 - PP_1843 0.67 +0.1 2,066,973 + PP_1843 0.69 -2.2 2,066,973 + PP_1843 0.69 +0.1 2,066,974 - PP_1843 0.69 +0.7 2,066,974 - PP_1843 0.69 -1.1 2,066,974 - PP_1843 0.69 -1.1 2,067,020 + PP_1843 0.74 -1.1 2,067,020 - PP_1843 0.74 +0.4 2,067,245 + +0.0
Or see this region's nucleotide sequence