Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1698 and PP_1699 are separated by 15 nucleotides PP_1699 and PP_1700 are separated by 49 nucleotides PP_1700 and PP_1701 are separated by 3 nucleotides PP_1701 and PP_1702 are separated by 217 nucleotides
PP_1698: PP_1698 - Major facilitator family transporter, at 1,892,585 to 1,893,859
_1698
PP_1699: PP_1699 - 2-pyrone-4,6-dicarboxylic acid hydrolase, at 1,893,875 to 1,894,717
_1699
PP_1700: PP_1700 - Acyltransferase, at 1,894,767 to 1,896,752
_1700
PP_1701: PP_1701 - transporter, at 1,896,756 to 1,897,073
_1701
PP_1702: PP_1702 - Recombination-associated protein RdgC, at 1,897,291 to 1,898,211
_1702
Position (kb)
1894
1895
1896
1897 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1893.857 kb on + strand at 1893.858 kb on - strand at 1893.874 kb on + strand at 1893.874 kb on + strand at 1893.874 kb on + strand at 1893.874 kb on + strand at 1893.875 kb on - strand at 1893.986 kb on + strand, within PP_1699 at 1893.986 kb on + strand, within PP_1699 at 1893.987 kb on - strand, within PP_1699 at 1894.047 kb on + strand, within PP_1699 at 1894.048 kb on - strand, within PP_1699 at 1894.048 kb on - strand, within PP_1699 at 1894.201 kb on - strand, within PP_1699 at 1894.478 kb on + strand, within PP_1699 at 1894.479 kb on - strand, within PP_1699 at 1894.479 kb on - strand, within PP_1699 at 1894.550 kb on + strand, within PP_1699 at 1894.720 kb on + strand at 1894.721 kb on - strand at 1894.763 kb on + strand at 1895.062 kb on + strand, within PP_1700 at 1895.266 kb on - strand, within PP_1700 at 1895.394 kb on + strand, within PP_1700 at 1895.395 kb on - strand, within PP_1700 at 1895.395 kb on - strand, within PP_1700 at 1895.395 kb on - strand, within PP_1700 at 1895.443 kb on - strand, within PP_1700 at 1895.613 kb on + strand, within PP_1700 at 1895.613 kb on + strand, within PP_1700 at 1895.614 kb on - strand, within PP_1700 at 1895.635 kb on - strand, within PP_1700 at 1895.827 kb on + strand, within PP_1700 at 1895.827 kb on + strand, within PP_1700 at 1895.931 kb on + strand, within PP_1700 at 1895.938 kb on + strand, within PP_1700 at 1895.939 kb on - strand, within PP_1700 at 1895.946 kb on + strand, within PP_1700 at 1895.947 kb on - strand, within PP_1700 at 1895.947 kb on - strand, within PP_1700 at 1895.947 kb on - strand, within PP_1700 at 1896.061 kb on + strand, within PP_1700 at 1896.132 kb on + strand, within PP_1700 at 1896.132 kb on + strand, within PP_1700 at 1896.132 kb on + strand, within PP_1700 at 1896.132 kb on + strand, within PP_1700 at 1896.133 kb on - strand, within PP_1700 at 1896.133 kb on - strand, within PP_1700 at 1896.135 kb on - strand, within PP_1700 at 1896.135 kb on - strand, within PP_1700 at 1896.199 kb on - strand, within PP_1700 at 1896.303 kb on - strand, within PP_1700 at 1896.330 kb on + strand, within PP_1700 at 1896.333 kb on + strand, within PP_1700 at 1896.334 kb on - strand, within PP_1700 at 1896.334 kb on - strand, within PP_1700 at 1896.334 kb on - strand, within PP_1700 at 1896.334 kb on - strand, within PP_1700 at 1896.334 kb on - strand, within PP_1700 at 1896.342 kb on + strand, within PP_1700 at 1896.342 kb on + strand, within PP_1700 at 1896.342 kb on + strand, within PP_1700 at 1896.342 kb on + strand, within PP_1700 at 1896.342 kb on + strand, within PP_1700 at 1896.342 kb on + strand, within PP_1700 at 1896.343 kb on - strand, within PP_1700 at 1896.424 kb on - strand, within PP_1700 at 1896.424 kb on - strand at 1896.424 kb on - strand, within PP_1700 at 1896.424 kb on - strand, within PP_1700 at 1896.453 kb on + strand, within PP_1700 at 1896.518 kb on - strand, within PP_1700 at 1896.602 kb on + strand at 1896.603 kb on - strand at 1896.971 kb on + strand, within PP_1701 at 1897.286 kb on - strand at 1897.643 kb on + strand at 1897.643 kb on + strand, within PP_1702 at 1897.643 kb on + strand, within PP_1702 at 1897.643 kb on + strand, within PP_1702 at 1897.644 kb on - strand at 1897.644 kb on - strand, within PP_1702 at 1897.644 kb on - strand, within PP_1702
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 1,893,857 + -0.5 1,893,858 - -0.4 1,893,874 + +1.1 1,893,874 + +0.4 1,893,874 + +1.6 1,893,874 + +1.3 1,893,875 - +0.9 1,893,986 + PP_1699 0.13 +0.8 1,893,986 + PP_1699 0.13 +0.6 1,893,987 - PP_1699 0.13 +0.1 1,894,047 + PP_1699 0.20 +1.1 1,894,048 - PP_1699 0.21 +0.1 1,894,048 - PP_1699 0.21 -2.4 1,894,201 - PP_1699 0.39 +0.3 1,894,478 + PP_1699 0.72 +0.1 1,894,479 - PP_1699 0.72 -0.8 1,894,479 - PP_1699 0.72 +0.3 1,894,550 + PP_1699 0.80 +0.6 1,894,720 + -1.3 1,894,721 - +0.3 1,894,763 + +1.4 1,895,062 + PP_1700 0.15 -0.7 1,895,266 - PP_1700 0.25 +1.1 1,895,394 + PP_1700 0.32 -1.3 1,895,395 - PP_1700 0.32 -1.2 1,895,395 - PP_1700 0.32 -0.5 1,895,395 - PP_1700 0.32 +0.9 1,895,443 - PP_1700 0.34 -0.1 1,895,613 + PP_1700 0.43 +0.5 1,895,613 + PP_1700 0.43 -0.6 1,895,614 - PP_1700 0.43 -0.3 1,895,635 - PP_1700 0.44 -0.0 1,895,827 + PP_1700 0.53 +1.3 1,895,827 + PP_1700 0.53 -0.3 1,895,931 + PP_1700 0.59 +0.4 1,895,938 + PP_1700 0.59 -0.5 1,895,939 - PP_1700 0.59 -0.2 1,895,946 + PP_1700 0.59 -1.1 1,895,947 - PP_1700 0.59 +0.2 1,895,947 - PP_1700 0.59 -0.5 1,895,947 - PP_1700 0.59 +0.7 1,896,061 + PP_1700 0.65 +0.2 1,896,132 + PP_1700 0.69 +0.7 1,896,132 + PP_1700 0.69 -0.2 1,896,132 + PP_1700 0.69 -1.8 1,896,132 + PP_1700 0.69 +0.2 1,896,133 - PP_1700 0.69 -2.0 1,896,133 - PP_1700 0.69 -0.6 1,896,135 - PP_1700 0.69 -0.2 1,896,135 - PP_1700 0.69 +0.2 1,896,199 - PP_1700 0.72 -0.2 1,896,303 - PP_1700 0.77 -0.2 1,896,330 + PP_1700 0.79 -0.0 1,896,333 + PP_1700 0.79 -1.5 1,896,334 - PP_1700 0.79 -2.1 1,896,334 - PP_1700 0.79 -0.4 1,896,334 - PP_1700 0.79 -1.1 1,896,334 - PP_1700 0.79 +0.3 1,896,334 - PP_1700 0.79 +2.4 1,896,342 + PP_1700 0.79 +1.2 1,896,342 + PP_1700 0.79 -1.1 1,896,342 + PP_1700 0.79 -0.1 1,896,342 + PP_1700 0.79 -0.5 1,896,342 + PP_1700 0.79 +0.0 1,896,342 + PP_1700 0.79 -1.7 1,896,343 - PP_1700 0.79 -0.9 1,896,424 - PP_1700 0.83 +0.4 1,896,424 - +1.7 1,896,424 - PP_1700 0.83 +0.1 1,896,424 - PP_1700 0.83 -3.1 1,896,453 + PP_1700 0.85 -1.0 1,896,518 - PP_1700 0.88 -0.1 1,896,602 + +0.4 1,896,603 - +0.9 1,896,971 + PP_1701 0.68 +0.2 1,897,286 - +1.0 1,897,643 + +3.0 1,897,643 + PP_1702 0.38 -1.5 1,897,643 + PP_1702 0.38 -1.2 1,897,643 + PP_1702 0.38 +1.0 1,897,644 - -1.9 1,897,644 - PP_1702 0.38 -1.0 1,897,644 - PP_1702 0.38 -1.9
Or see this region's nucleotide sequence