Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0850 and PP_0851 are separated by 8 nucleotides PP_0851 and PP_0852 overlap by 4 nucleotides PP_0852 and PP_0853 are separated by 3 nucleotides
PP_0850: PP_0850 - Dual-specificity RNA methyltransferase RlmN, at 984,085 to 985,230
_0850
PP_0851: PP_0851 - Type IV pili biogenesis protein PilF, at 985,239 to 986,006
_0851
PP_0852: PP_0852 - Transcriptional regulator, Cro/CI family, at 986,003 to 987,010
_0852
PP_0853: PP_0853 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, at 987,014 to 988,123
_0853
Position (kb) 986
987
988 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 985.086 kb on + strand, within PP_0850 at 985.149 kb on + strand at 985.168 kb on + strand at 985.169 kb on - strand at 985.169 kb on - strand at 985.169 kb on - strand at 985.235 kb on + strand at 985.236 kb on - strand at 985.236 kb on - strand at 985.362 kb on + strand, within PP_0851 at 985.362 kb on + strand, within PP_0851 at 985.362 kb on + strand, within PP_0851 at 985.363 kb on - strand, within PP_0851 at 985.363 kb on - strand, within PP_0851 at 985.363 kb on - strand, within PP_0851 at 985.384 kb on - strand, within PP_0851 at 985.543 kb on + strand, within PP_0851 at 985.543 kb on + strand, within PP_0851 at 985.543 kb on + strand, within PP_0851 at 985.544 kb on - strand, within PP_0851 at 985.576 kb on - strand, within PP_0851 at 985.591 kb on - strand, within PP_0851 at 985.653 kb on + strand, within PP_0851 at 985.731 kb on + strand, within PP_0851 at 985.731 kb on + strand, within PP_0851 at 985.731 kb on + strand, within PP_0851 at 985.815 kb on + strand, within PP_0851 at 985.815 kb on + strand, within PP_0851 at 985.815 kb on + strand, within PP_0851 at 985.815 kb on + strand, within PP_0851 at 985.815 kb on + strand, within PP_0851 at 985.815 kb on + strand, within PP_0851 at 985.815 kb on + strand, within PP_0851 at 985.815 kb on + strand, within PP_0851 at 985.815 kb on + strand, within PP_0851 at 985.815 kb on + strand, within PP_0851 at 985.833 kb on + strand, within PP_0851 at 985.833 kb on + strand, within PP_0851 at 985.833 kb on + strand, within PP_0851 at 985.836 kb on + strand, within PP_0851 at 985.836 kb on + strand, within PP_0851 at 985.836 kb on + strand, within PP_0851 at 985.836 kb on + strand, within PP_0851 at 985.836 kb on + strand, within PP_0851 at 985.948 kb on + strand at 985.959 kb on + strand at 985.986 kb on + strand at 985.986 kb on + strand at 986.025 kb on + strand at 986.025 kb on + strand at 986.110 kb on + strand, within PP_0852 at 986.110 kb on + strand, within PP_0852 at 986.179 kb on + strand, within PP_0852 at 986.195 kb on + strand, within PP_0852 at 986.244 kb on + strand, within PP_0852 at 986.244 kb on + strand, within PP_0852 at 986.338 kb on + strand, within PP_0852 at 986.338 kb on + strand, within PP_0852 at 986.939 kb on + strand at 986.939 kb on + strand at 986.939 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 985,086 + PP_0850 0.87 -0.6 985,149 + -0.6 985,168 + -0.3 985,169 - -1.0 985,169 - -3.3 985,169 - -2.0 985,235 + +0.9 985,236 - -3.5 985,236 - -2.2 985,362 + PP_0851 0.16 +1.2 985,362 + PP_0851 0.16 +0.6 985,362 + PP_0851 0.16 +2.1 985,363 - PP_0851 0.16 +0.2 985,363 - PP_0851 0.16 -2.3 985,363 - PP_0851 0.16 -2.4 985,384 - PP_0851 0.19 +0.4 985,543 + PP_0851 0.40 +0.6 985,543 + PP_0851 0.40 +1.5 985,543 + PP_0851 0.40 -1.2 985,544 - PP_0851 0.40 -2.6 985,576 - PP_0851 0.44 +0.3 985,591 - PP_0851 0.46 -2.1 985,653 + PP_0851 0.54 +1.5 985,731 + PP_0851 0.64 +1.0 985,731 + PP_0851 0.64 +1.4 985,731 + PP_0851 0.64 +2.1 985,815 + PP_0851 0.75 -0.4 985,815 + PP_0851 0.75 +1.0 985,815 + PP_0851 0.75 +1.0 985,815 + PP_0851 0.75 +1.8 985,815 + PP_0851 0.75 +1.2 985,815 + PP_0851 0.75 +0.6 985,815 + PP_0851 0.75 +1.5 985,815 + PP_0851 0.75 +0.7 985,815 + PP_0851 0.75 +1.3 985,815 + PP_0851 0.75 -0.1 985,833 + PP_0851 0.77 +1.5 985,833 + PP_0851 0.77 +1.2 985,833 + PP_0851 0.77 +2.2 985,836 + PP_0851 0.78 +0.9 985,836 + PP_0851 0.78 +0.7 985,836 + PP_0851 0.78 +1.2 985,836 + PP_0851 0.78 +1.2 985,836 + PP_0851 0.78 +0.8 985,948 + +1.1 985,959 + +0.5 985,986 + -0.6 985,986 + -2.6 986,025 + -1.6 986,025 + -3.3 986,110 + PP_0852 0.11 -2.8 986,110 + PP_0852 0.11 -2.5 986,179 + PP_0852 0.17 -3.3 986,195 + PP_0852 0.19 -2.6 986,244 + PP_0852 0.24 -1.7 986,244 + PP_0852 0.24 -3.2 986,338 + PP_0852 0.33 -3.6 986,338 + PP_0852 0.33 -1.5 986,939 + -1.5 986,939 + -3.7 986,939 + -3.8
Or see this region's nucleotide sequence