Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0839 and PP_0840 are separated by 3 nucleotides PP_0840 and PP_0841 are separated by 179 nucleotides PP_0841 and PP_0842 are separated by 45 nucleotides
PP_0839: PP_0839 - tRNA Cm32/Um32 methyltransferase, at 976,290 to 977,045
_0839
PP_0840: PP_0840 - serine acetyltransferase, at 977,049 to 977,834
_0840
PP_0841: PP_0841 - DNA-binding transcriptional dual regulator 2Fe-2S cluster IscR, at 978,014 to 978,505
_0841
PP_0842: PP_0842 - cysteine desulfurase, at 978,551 to 979,765
_0842
Position (kb) 978
979 Strain fitness (log2 ratio) -2
-1
0
1
2 at 977.072 kb on + strand at 977.073 kb on - strand at 977.083 kb on + strand at 977.084 kb on - strand at 977.298 kb on - strand, within PP_0840 at 977.522 kb on + strand, within PP_0840 at 977.588 kb on + strand, within PP_0840 at 977.622 kb on + strand, within PP_0840 at 977.714 kb on + strand, within PP_0840 at 977.715 kb on - strand, within PP_0840 at 977.715 kb on - strand, within PP_0840 at 977.715 kb on - strand, within PP_0840 at 977.715 kb on - strand, within PP_0840 at 977.748 kb on + strand, within PP_0840 at 977.748 kb on + strand, within PP_0840 at 977.749 kb on - strand, within PP_0840 at 977.749 kb on - strand, within PP_0840 at 977.749 kb on - strand, within PP_0840 at 977.749 kb on - strand, within PP_0840 at 978.009 kb on + strand at 978.206 kb on + strand, within PP_0841 at 978.384 kb on + strand, within PP_0841 at 978.384 kb on + strand, within PP_0841 at 978.384 kb on + strand, within PP_0841 at 978.384 kb on + strand, within PP_0841 at 978.384 kb on + strand, within PP_0841 at 978.539 kb on + strand at 978.539 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 977,072 + +1.2 977,073 - +0.2 977,083 + +0.1 977,084 - +1.2 977,298 - PP_0840 0.32 -1.4 977,522 + PP_0840 0.60 +0.2 977,588 + PP_0840 0.69 +0.3 977,622 + PP_0840 0.73 -1.2 977,714 + PP_0840 0.85 -0.8 977,715 - PP_0840 0.85 +0.0 977,715 - PP_0840 0.85 +0.3 977,715 - PP_0840 0.85 -1.5 977,715 - PP_0840 0.85 +0.8 977,748 + PP_0840 0.89 -0.8 977,748 + PP_0840 0.89 -0.3 977,749 - PP_0840 0.89 -0.0 977,749 - PP_0840 0.89 -2.2 977,749 - PP_0840 0.89 +1.1 977,749 - PP_0840 0.89 +0.6 978,009 + +0.8 978,206 + PP_0841 0.39 +1.4 978,384 + PP_0841 0.75 +2.1 978,384 + PP_0841 0.75 +0.8 978,384 + PP_0841 0.75 +1.5 978,384 + PP_0841 0.75 +1.1 978,384 + PP_0841 0.75 +2.0 978,539 + -0.2 978,539 + -0.8
Or see this region's nucleotide sequence