Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0257 and PP_0258 are separated by 175 nucleotides PP_0258 and PP_0259 are separated by 50 nucleotides PP_0259 and PP_0260 are separated by 64 nucleotides PP_0260 and PP_0261 are separated by 14 nucleotides
PP_0257: PP_0257 - protein sulfur transferase, at 312,381 to 313,211
_0257
PP_0258: PP_0258 - regulator of murein cross-linking, at 313,387 to 313,827
_0258
PP_0259: PP_0259 - purine nucleotidase, at 313,878 to 314,540
_0259
PP_0260: PP_0260 - ADP-sugar pyrophosphorylase, at 314,605 to 315,171
_0260
PP_0261: PP_0261 - adenosine-3'(2'),5'-bisphosphate nucleotidase, at 315,186 to 315,986
_0261
Position (kb) 313
314
315 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 312.925 kb on + strand, within PP_0257 at 312.926 kb on - strand, within PP_0257 at 312.926 kb on - strand, within PP_0257 at 312.926 kb on - strand, within PP_0257 at 313.069 kb on + strand, within PP_0257 at 313.069 kb on + strand, within PP_0257 at 313.069 kb on + strand, within PP_0257 at 313.069 kb on + strand, within PP_0257 at 313.070 kb on - strand, within PP_0257 at 313.070 kb on - strand, within PP_0257 at 313.139 kb on - strand at 313.144 kb on + strand at 313.144 kb on + strand at 313.145 kb on - strand at 313.145 kb on - strand at 313.241 kb on + strand at 313.289 kb on + strand at 313.290 kb on - strand at 313.299 kb on + strand at 313.300 kb on - strand at 313.300 kb on - strand at 313.336 kb on - strand at 313.472 kb on + strand, within PP_0258 at 313.472 kb on + strand, within PP_0258 at 313.473 kb on - strand at 313.527 kb on - strand, within PP_0258 at 313.723 kb on + strand, within PP_0258 at 313.724 kb on - strand, within PP_0258 at 313.741 kb on + strand, within PP_0258 at 313.742 kb on - strand, within PP_0258 at 313.841 kb on + strand at 313.841 kb on + strand at 313.879 kb on + strand at 313.880 kb on - strand at 313.887 kb on + strand at 313.893 kb on + strand at 314.110 kb on + strand, within PP_0259 at 314.110 kb on + strand, within PP_0259 at 314.111 kb on - strand, within PP_0259 at 314.140 kb on + strand, within PP_0259 at 314.140 kb on + strand, within PP_0259 at 314.140 kb on + strand, within PP_0259 at 314.140 kb on + strand, within PP_0259 at 314.141 kb on - strand, within PP_0259 at 314.141 kb on - strand, within PP_0259 at 314.141 kb on - strand, within PP_0259 at 314.247 kb on - strand, within PP_0259 at 314.313 kb on + strand, within PP_0259 at 314.313 kb on + strand, within PP_0259 at 314.314 kb on - strand, within PP_0259 at 314.314 kb on - strand, within PP_0259 at 314.341 kb on + strand, within PP_0259 at 314.410 kb on - strand, within PP_0259 at 314.467 kb on + strand, within PP_0259 at 314.468 kb on - strand, within PP_0259 at 314.468 kb on - strand, within PP_0259 at 314.572 kb on + strand at 314.572 kb on + strand at 314.572 kb on + strand at 314.572 kb on + strand at 314.573 kb on - strand at 314.573 kb on - strand at 314.574 kb on + strand at 314.574 kb on + strand at 314.574 kb on + strand at 314.574 kb on + strand at 314.574 kb on + strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.577 kb on + strand at 314.624 kb on + strand at 314.675 kb on + strand, within PP_0260 at 314.675 kb on + strand, within PP_0260 at 314.710 kb on + strand, within PP_0260 at 314.710 kb on + strand, within PP_0260 at 314.710 kb on + strand, within PP_0260 at 314.710 kb on + strand, within PP_0260 at 314.711 kb on - strand, within PP_0260 at 314.711 kb on - strand, within PP_0260 at 314.711 kb on - strand, within PP_0260 at 314.711 kb on - strand, within PP_0260 at 314.800 kb on + strand, within PP_0260 at 314.801 kb on - strand, within PP_0260 at 314.821 kb on + strand, within PP_0260 at 314.822 kb on - strand, within PP_0260 at 314.822 kb on - strand, within PP_0260 at 314.959 kb on + strand, within PP_0260 at 314.960 kb on - strand, within PP_0260 at 314.960 kb on - strand, within PP_0260 at 314.960 kb on - strand, within PP_0260 at 314.960 kb on - strand, within PP_0260 at 315.167 kb on + strand at 315.167 kb on + strand at 315.167 kb on + strand at 315.167 kb on + strand at 315.167 kb on + strand at 315.167 kb on + strand at 315.167 kb on + strand at 315.168 kb on - strand at 315.301 kb on + strand, within PP_0261 at 315.380 kb on + strand, within PP_0261 at 315.380 kb on + strand, within PP_0261 at 315.380 kb on + strand, within PP_0261 at 315.381 kb on - strand, within PP_0261 at 315.381 kb on - strand, within PP_0261 at 315.381 kb on - strand, within PP_0261 at 315.381 kb on - strand, within PP_0261
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 312,925 + PP_0257 0.65 +0.3 312,926 - PP_0257 0.66 +0.8 312,926 - PP_0257 0.66 +0.3 312,926 - PP_0257 0.66 -1.2 313,069 + PP_0257 0.83 -2.2 313,069 + PP_0257 0.83 +0.1 313,069 + PP_0257 0.83 -0.7 313,069 + PP_0257 0.83 -0.9 313,070 - PP_0257 0.83 -0.6 313,070 - PP_0257 0.83 -0.5 313,139 - -0.4 313,144 + -0.9 313,144 + -0.0 313,145 - +0.5 313,145 - -1.0 313,241 + +0.2 313,289 + -2.1 313,290 - -0.7 313,299 + +0.4 313,300 - -0.3 313,300 - -2.3 313,336 - +1.5 313,472 + PP_0258 0.19 -0.7 313,472 + PP_0258 0.19 +0.3 313,473 - -0.2 313,527 - PP_0258 0.32 -0.3 313,723 + PP_0258 0.76 -1.0 313,724 - PP_0258 0.76 -0.3 313,741 + PP_0258 0.80 -2.2 313,742 - PP_0258 0.80 -1.4 313,841 + +0.1 313,841 + -2.1 313,879 + -1.2 313,880 - +1.0 313,887 + -1.0 313,893 + +1.2 314,110 + PP_0259 0.35 +1.8 314,110 + PP_0259 0.35 -0.3 314,111 - PP_0259 0.35 +0.0 314,140 + PP_0259 0.40 +1.1 314,140 + PP_0259 0.40 -0.2 314,140 + PP_0259 0.40 +0.2 314,140 + PP_0259 0.40 +1.4 314,141 - PP_0259 0.40 +1.6 314,141 - PP_0259 0.40 +1.1 314,141 - PP_0259 0.40 +0.9 314,247 - PP_0259 0.56 +0.8 314,313 + PP_0259 0.66 -1.4 314,313 + PP_0259 0.66 -0.8 314,314 - PP_0259 0.66 +0.9 314,314 - PP_0259 0.66 -0.7 314,341 + PP_0259 0.70 +0.1 314,410 - PP_0259 0.80 -0.2 314,467 + PP_0259 0.89 +1.7 314,468 - PP_0259 0.89 +0.8 314,468 - PP_0259 0.89 -1.9 314,572 + -0.1 314,572 + +2.0 314,572 + -2.0 314,572 + +0.4 314,573 - -2.2 314,573 - -0.2 314,574 + +0.5 314,574 + +0.1 314,574 + +0.8 314,574 + +0.8 314,574 + -1.7 314,575 - -0.0 314,575 - +1.1 314,575 - +0.5 314,575 - -0.6 314,575 - -1.8 314,575 - +1.9 314,575 - +1.5 314,577 + -0.0 314,624 + -1.8 314,675 + PP_0260 0.12 +1.2 314,675 + PP_0260 0.12 -2.7 314,710 + PP_0260 0.19 -0.1 314,710 + PP_0260 0.19 -1.0 314,710 + PP_0260 0.19 -0.0 314,710 + PP_0260 0.19 -1.7 314,711 - PP_0260 0.19 -2.1 314,711 - PP_0260 0.19 -0.8 314,711 - PP_0260 0.19 -0.9 314,711 - PP_0260 0.19 -0.9 314,800 + PP_0260 0.34 +0.4 314,801 - PP_0260 0.35 -0.8 314,821 + PP_0260 0.38 -2.2 314,822 - PP_0260 0.38 -1.1 314,822 - PP_0260 0.38 +0.2 314,959 + PP_0260 0.62 -0.8 314,960 - PP_0260 0.63 -1.2 314,960 - PP_0260 0.63 -1.4 314,960 - PP_0260 0.63 -0.9 314,960 - PP_0260 0.63 -1.7 315,167 + -0.3 315,167 + +0.5 315,167 + -1.4 315,167 + -0.4 315,167 + -0.3 315,167 + +0.2 315,167 + +0.2 315,168 - -0.6 315,301 + PP_0261 0.14 -0.8 315,380 + PP_0261 0.24 -2.8 315,380 + PP_0261 0.24 -3.4 315,380 + PP_0261 0.24 +1.7 315,381 - PP_0261 0.24 -3.2 315,381 - PP_0261 0.24 -3.0 315,381 - PP_0261 0.24 -1.6 315,381 - PP_0261 0.24 -0.4
Or see this region's nucleotide sequence