Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5045 and PP_5046 are separated by 470 nucleotides PP_5046 and PP_5047 are separated by 365 nucleotides
PP_5045: PP_5045 - tRNA sulfurtransferase, at 5,750,108 to 5,751,562
_5045
PP_5046: PP_5046 - glutamine synthetase, at 5,752,033 to 5,753,439
_5046
PP_5047: PP_5047 - two-component system sensory histidine kinase/phosphatase GlnL/GlnG, at 5,753,805 to 5,754,890
_5047
Position (kb)
5752
5753
5754 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5751.079 kb on - strand, within PP_5045 at 5751.178 kb on - strand, within PP_5045 at 5751.178 kb on - strand, within PP_5045 at 5751.320 kb on + strand, within PP_5045 at 5751.568 kb on - strand at 5751.610 kb on - strand at 5751.661 kb on + strand at 5751.662 kb on - strand at 5751.662 kb on - strand at 5751.662 kb on - strand at 5751.662 kb on - strand at 5751.723 kb on - strand at 5751.772 kb on - strand at 5751.804 kb on + strand at 5751.835 kb on + strand at 5751.836 kb on - strand at 5751.836 kb on - strand at 5751.892 kb on - strand at 5751.892 kb on - strand at 5752.013 kb on + strand at 5753.546 kb on + strand at 5753.662 kb on - strand at 5753.762 kb on - strand at 5753.804 kb on + strand at 5753.804 kb on + strand at 5753.804 kb on + strand at 5753.892 kb on + strand at 5753.892 kb on + strand at 5753.892 kb on + strand at 5753.893 kb on - strand at 5754.050 kb on + strand, within PP_5047 at 5754.051 kb on - strand, within PP_5047 at 5754.092 kb on - strand, within PP_5047 at 5754.274 kb on + strand, within PP_5047 at 5754.312 kb on + strand, within PP_5047 at 5754.313 kb on - strand, within PP_5047 at 5754.313 kb on - strand, within PP_5047 at 5754.407 kb on + strand, within PP_5047
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 5,751,079 - PP_5045 0.67 -2.7 5,751,178 - PP_5045 0.74 -1.9 5,751,178 - PP_5045 0.74 -2.8 5,751,320 + PP_5045 0.83 -2.3 5,751,568 - -0.8 5,751,610 - -1.0 5,751,661 + -1.4 5,751,662 - -1.8 5,751,662 - +0.3 5,751,662 - -0.6 5,751,662 - -0.8 5,751,723 - -1.4 5,751,772 - +0.6 5,751,804 + -1.8 5,751,835 + +0.1 5,751,836 - +0.1 5,751,836 - -0.2 5,751,892 - -1.5 5,751,892 - -0.4 5,752,013 + +1.6 5,753,546 + +0.6 5,753,662 - -0.2 5,753,762 - +0.4 5,753,804 + -0.8 5,753,804 + -2.1 5,753,804 + -0.6 5,753,892 + -1.6 5,753,892 + +1.4 5,753,892 + -0.3 5,753,893 - -0.0 5,754,050 + PP_5047 0.23 +2.8 5,754,051 - PP_5047 0.23 +0.4 5,754,092 - PP_5047 0.26 -1.9 5,754,274 + PP_5047 0.43 -1.1 5,754,312 + PP_5047 0.47 -1.4 5,754,313 - PP_5047 0.47 +0.1 5,754,313 - PP_5047 0.47 -0.3 5,754,407 + PP_5047 0.55 -1.0
Or see this region's nucleotide sequence