Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3242 and PP_3243 are separated by 42 nucleotides PP_3243 and PP_3244 are separated by 17 nucleotides PP_3244 and PP_3245 are separated by 106 nucleotides
PP_3242: PP_3242 - GGDEF domain protein, at 3,676,998 to 3,678,566
_3242
PP_3243: PP_3243 - Acetyltransferase, GNAT family, at 3,678,609 to 3,679,118
_3243
PP_3244: PP_3244 - Magnesium transporter, MgtC family, at 3,679,136 to 3,679,849
_3244
PP_3245: PP_3245 - conserved protein of unknown function, at 3,679,956 to 3,680,234
_3245
Position (kb)
3678
3679
3680 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3677.613 kb on + strand at 3677.614 kb on - strand, within PP_3242 at 3677.711 kb on - strand, within PP_3242 at 3677.711 kb on - strand, within PP_3242 at 3677.757 kb on + strand, within PP_3242 at 3677.757 kb on + strand, within PP_3242 at 3677.758 kb on - strand, within PP_3242 at 3677.814 kb on + strand, within PP_3242 at 3677.814 kb on + strand, within PP_3242 at 3677.814 kb on + strand, within PP_3242 at 3678.056 kb on + strand, within PP_3242 at 3678.056 kb on + strand, within PP_3242 at 3678.057 kb on - strand, within PP_3242 at 3678.057 kb on - strand, within PP_3242 at 3678.057 kb on - strand, within PP_3242 at 3678.057 kb on - strand, within PP_3242 at 3678.163 kb on - strand, within PP_3242 at 3678.312 kb on - strand, within PP_3242 at 3678.349 kb on + strand, within PP_3242 at 3678.350 kb on - strand, within PP_3242 at 3678.458 kb on + strand at 3678.736 kb on + strand, within PP_3243 at 3678.789 kb on + strand, within PP_3243 at 3678.959 kb on + strand, within PP_3243 at 3678.959 kb on + strand, within PP_3243 at 3679.103 kb on + strand at 3679.138 kb on - strand at 3679.163 kb on + strand at 3679.164 kb on - strand at 3679.184 kb on + strand at 3679.184 kb on + strand at 3679.184 kb on + strand at 3679.184 kb on + strand at 3679.185 kb on - strand at 3679.185 kb on - strand at 3679.254 kb on - strand, within PP_3244 at 3679.254 kb on - strand, within PP_3244 at 3679.254 kb on - strand, within PP_3244 at 3679.590 kb on - strand, within PP_3244 at 3679.650 kb on + strand, within PP_3244 at 3679.651 kb on - strand, within PP_3244 at 3679.909 kb on + strand at 3679.909 kb on + strand at 3679.958 kb on - strand at 3680.049 kb on + strand, within PP_3245 at 3680.102 kb on + strand, within PP_3245
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 3,677,613 + +0.8 3,677,614 - PP_3242 0.39 -0.6 3,677,711 - PP_3242 0.45 -0.4 3,677,711 - PP_3242 0.45 +0.3 3,677,757 + PP_3242 0.48 +1.3 3,677,757 + PP_3242 0.48 +0.1 3,677,758 - PP_3242 0.48 +0.1 3,677,814 + PP_3242 0.52 -0.4 3,677,814 + PP_3242 0.52 -0.2 3,677,814 + PP_3242 0.52 +0.1 3,678,056 + PP_3242 0.67 +0.1 3,678,056 + PP_3242 0.67 +0.8 3,678,057 - PP_3242 0.67 +0.2 3,678,057 - PP_3242 0.67 -0.1 3,678,057 - PP_3242 0.67 +0.1 3,678,057 - PP_3242 0.67 -0.4 3,678,163 - PP_3242 0.74 -1.5 3,678,312 - PP_3242 0.84 +0.8 3,678,349 + PP_3242 0.86 +0.3 3,678,350 - PP_3242 0.86 +0.0 3,678,458 + +0.7 3,678,736 + PP_3243 0.25 +0.4 3,678,789 + PP_3243 0.35 -0.4 3,678,959 + PP_3243 0.69 +0.3 3,678,959 + PP_3243 0.69 -0.9 3,679,103 + -3.3 3,679,138 - -1.8 3,679,163 + -2.2 3,679,164 - -4.0 3,679,184 + -2.2 3,679,184 + -3.8 3,679,184 + -3.7 3,679,184 + -4.8 3,679,185 - -4.1 3,679,185 - -3.7 3,679,254 - PP_3244 0.17 -3.2 3,679,254 - PP_3244 0.17 -3.1 3,679,254 - PP_3244 0.17 -3.1 3,679,590 - PP_3244 0.64 -3.5 3,679,650 + PP_3244 0.72 -2.0 3,679,651 - PP_3244 0.72 -2.9 3,679,909 + -2.5 3,679,909 + -3.3 3,679,958 - -3.0 3,680,049 + PP_3245 0.33 -2.9 3,680,102 + PP_3245 0.52 -3.7
Or see this region's nucleotide sequence