Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0648 and PP_0649 are separated by 87 nucleotides PP_0649 and PP_0650 are separated by 27 nucleotides PP_0650 and PP_0651 are separated by 108 nucleotides PP_0651 and PP_5453 are separated by 833 nucleotides
PP_0648: PP_0648 - conserved protein of unknown function, at 757,379 to 757,834
_0648
PP_0649: PP_0649 - MutT/nudix family protein, at 757,922 to 758,332
_0649
PP_0650: PP_0650 - conserved protein of unknown function, at 758,360 to 758,470
_0650
PP_0651: PP_0651 - Acetyltransferase, GNAT family, at 758,579 to 759,028
_0651
PP_5453: PP_5453 - conserved protein of unknown function, at 759,862 to 760,089
_5453
Position (kb) 758
759
760 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 757.594 kb on + strand, within PP_0648 at 757.595 kb on - strand, within PP_0648 at 757.606 kb on + strand, within PP_0648 at 757.606 kb on + strand, within PP_0648 at 757.607 kb on - strand, within PP_0648 at 757.607 kb on - strand, within PP_0648 at 757.607 kb on - strand, within PP_0648 at 757.607 kb on - strand, within PP_0648 at 757.614 kb on - strand, within PP_0648 at 757.614 kb on - strand, within PP_0648 at 757.614 kb on - strand, within PP_0648 at 757.633 kb on + strand, within PP_0648 at 757.634 kb on - strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.639 kb on + strand, within PP_0648 at 757.639 kb on + strand, within PP_0648 at 757.640 kb on - strand, within PP_0648 at 757.640 kb on - strand, within PP_0648 at 757.641 kb on - strand, within PP_0648 at 757.673 kb on + strand, within PP_0648 at 757.674 kb on - strand, within PP_0648 at 757.674 kb on - strand, within PP_0648 at 757.693 kb on + strand, within PP_0648 at 757.756 kb on + strand, within PP_0648 at 757.756 kb on + strand, within PP_0648 at 757.757 kb on - strand, within PP_0648 at 757.757 kb on - strand, within PP_0648 at 757.767 kb on + strand, within PP_0648 at 757.767 kb on + strand, within PP_0648 at 757.803 kb on - strand at 757.838 kb on + strand at 757.880 kb on + strand at 757.880 kb on + strand at 757.880 kb on + strand at 757.900 kb on + strand at 757.900 kb on + strand at 757.900 kb on + strand at 757.947 kb on + strand at 757.947 kb on + strand at 757.948 kb on - strand at 757.948 kb on - strand at 757.948 kb on - strand at 757.948 kb on - strand at 757.948 kb on - strand at 757.980 kb on + strand, within PP_0649 at 757.998 kb on + strand, within PP_0649 at 757.998 kb on + strand, within PP_0649 at 757.999 kb on - strand, within PP_0649 at 758.087 kb on - strand, within PP_0649 at 758.087 kb on - strand, within PP_0649 at 758.189 kb on - strand, within PP_0649 at 758.189 kb on - strand, within PP_0649 at 758.306 kb on - strand at 758.330 kb on + strand at 758.331 kb on - strand at 758.331 kb on - strand at 758.331 kb on - strand at 758.331 kb on - strand at 758.340 kb on + strand at 758.340 kb on + strand at 758.341 kb on - strand at 758.341 kb on - strand at 758.358 kb on - strand at 758.384 kb on + strand, within PP_0650 at 758.384 kb on + strand, within PP_0650 at 758.385 kb on - strand, within PP_0650 at 758.385 kb on - strand, within PP_0650 at 758.387 kb on + strand, within PP_0650 at 758.387 kb on + strand, within PP_0650 at 758.388 kb on - strand, within PP_0650 at 758.388 kb on - strand, within PP_0650 at 758.388 kb on - strand, within PP_0650 at 758.388 kb on - strand, within PP_0650 at 758.446 kb on - strand, within PP_0650 at 758.446 kb on - strand, within PP_0650 at 758.451 kb on + strand, within PP_0650 at 758.451 kb on + strand, within PP_0650 at 758.451 kb on + strand, within PP_0650 at 758.452 kb on - strand, within PP_0650 at 758.452 kb on - strand, within PP_0650 at 758.486 kb on + strand at 758.486 kb on + strand at 758.486 kb on + strand at 758.514 kb on + strand at 758.514 kb on + strand at 758.515 kb on - strand at 758.538 kb on + strand at 758.539 kb on - strand at 758.539 kb on - strand at 758.539 kb on - strand at 758.560 kb on - strand at 758.562 kb on - strand at 758.626 kb on - strand, within PP_0651 at 758.626 kb on - strand, within PP_0651 at 758.638 kb on + strand, within PP_0651 at 758.638 kb on + strand, within PP_0651 at 758.639 kb on - strand, within PP_0651 at 758.640 kb on + strand, within PP_0651 at 758.640 kb on + strand, within PP_0651 at 758.737 kb on + strand, within PP_0651 at 758.738 kb on - strand at 758.757 kb on + strand, within PP_0651 at 758.757 kb on + strand, within PP_0651 at 758.758 kb on - strand, within PP_0651 at 758.758 kb on - strand, within PP_0651 at 758.763 kb on + strand, within PP_0651 at 758.764 kb on - strand, within PP_0651 at 758.881 kb on + strand, within PP_0651 at 758.881 kb on + strand, within PP_0651 at 758.881 kb on + strand, within PP_0651 at 758.883 kb on + strand, within PP_0651 at 758.883 kb on + strand, within PP_0651 at 758.883 kb on + strand, within PP_0651 at 758.884 kb on - strand, within PP_0651 at 758.913 kb on + strand, within PP_0651 at 758.913 kb on + strand, within PP_0651 at 758.913 kb on + strand, within PP_0651 at 758.913 kb on + strand, within PP_0651 at 758.913 kb on + strand, within PP_0651 at 758.914 kb on - strand, within PP_0651 at 758.914 kb on - strand, within PP_0651 at 758.914 kb on - strand, within PP_0651 at 758.914 kb on - strand, within PP_0651 at 758.914 kb on - strand, within PP_0651 at 758.948 kb on + strand, within PP_0651 at 758.948 kb on + strand, within PP_0651 at 759.011 kb on - strand at 759.026 kb on + strand at 759.026 kb on + strand at 759.027 kb on - strand at 759.042 kb on + strand at 759.042 kb on + strand at 759.047 kb on + strand at 759.048 kb on - strand at 759.048 kb on - strand at 759.070 kb on - strand at 759.070 kb on - strand at 759.092 kb on + strand at 759.092 kb on + strand at 759.092 kb on + strand at 759.092 kb on + strand at 759.093 kb on - strand at 759.093 kb on - strand at 759.093 kb on - strand at 759.093 kb on - strand at 759.096 kb on + strand at 759.096 kb on + strand at 759.096 kb on + strand at 759.097 kb on - strand at 759.097 kb on - strand at 759.118 kb on + strand at 759.118 kb on + strand at 759.119 kb on - strand at 759.119 kb on - strand at 759.202 kb on + strand at 759.226 kb on + strand at 759.276 kb on + strand at 759.277 kb on - strand at 759.288 kb on + strand at 759.288 kb on + strand at 759.288 kb on + strand at 759.289 kb on - strand at 759.294 kb on + strand at 759.294 kb on + strand at 759.295 kb on - strand at 759.310 kb on + strand at 759.310 kb on + strand at 759.310 kb on + strand at 759.310 kb on + strand at 759.311 kb on - strand at 759.313 kb on + strand at 759.313 kb on + strand at 759.374 kb on + strand at 759.375 kb on - strand at 759.375 kb on - strand at 759.396 kb on + strand at 759.419 kb on + strand at 759.434 kb on - strand at 759.607 kb on + strand at 759.608 kb on - strand at 759.608 kb on - strand at 759.675 kb on + strand at 759.675 kb on + strand at 759.675 kb on + strand at 759.675 kb on + strand at 759.676 kb on - strand at 759.676 kb on - strand at 759.676 kb on - strand at 759.676 kb on - strand at 759.676 kb on - strand at 759.676 kb on - strand at 759.760 kb on - strand at 759.810 kb on + strand at 759.811 kb on - strand at 759.811 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 757,594 + PP_0648 0.47 +0.8 757,595 - PP_0648 0.47 -2.5 757,606 + PP_0648 0.50 -0.7 757,606 + PP_0648 0.50 +0.2 757,607 - PP_0648 0.50 +0.7 757,607 - PP_0648 0.50 -0.1 757,607 - PP_0648 0.50 -0.3 757,607 - PP_0648 0.50 -1.9 757,614 - PP_0648 0.52 -0.7 757,614 - PP_0648 0.52 -0.3 757,614 - PP_0648 0.52 +0.3 757,633 + PP_0648 0.56 +0.2 757,634 - PP_0648 0.56 -0.8 757,637 + PP_0648 0.57 +1.0 757,637 + PP_0648 0.57 -0.1 757,637 + PP_0648 0.57 -0.0 757,637 + PP_0648 0.57 -0.7 757,637 + PP_0648 0.57 -0.9 757,637 + PP_0648 0.57 +0.1 757,637 + PP_0648 0.57 +0.7 757,638 - PP_0648 0.57 +0.4 757,638 - PP_0648 0.57 +0.4 757,638 - PP_0648 0.57 -0.9 757,638 - PP_0648 0.57 -0.4 757,638 - PP_0648 0.57 -0.6 757,638 - PP_0648 0.57 -0.7 757,638 - PP_0648 0.57 -0.2 757,638 - PP_0648 0.57 +0.3 757,638 - PP_0648 0.57 +0.7 757,639 + PP_0648 0.57 -0.3 757,639 + PP_0648 0.57 -0.9 757,640 - PP_0648 0.57 +0.7 757,640 - PP_0648 0.57 -3.4 757,641 - PP_0648 0.57 -0.5 757,673 + PP_0648 0.64 +0.4 757,674 - PP_0648 0.65 -0.3 757,674 - PP_0648 0.65 +0.5 757,693 + PP_0648 0.69 -0.4 757,756 + PP_0648 0.83 -0.0 757,756 + PP_0648 0.83 +0.1 757,757 - PP_0648 0.83 -0.1 757,757 - PP_0648 0.83 +0.2 757,767 + PP_0648 0.85 +1.2 757,767 + PP_0648 0.85 -0.3 757,803 - +0.6 757,838 + -0.8 757,880 + -0.6 757,880 + -1.8 757,880 + +1.2 757,900 + -0.6 757,900 + -0.3 757,900 + +0.2 757,947 + +0.2 757,947 + +0.1 757,948 - +0.3 757,948 - -1.3 757,948 - -0.0 757,948 - -0.2 757,948 - -0.4 757,980 + PP_0649 0.14 -1.7 757,998 + PP_0649 0.18 +0.7 757,998 + PP_0649 0.18 +0.1 757,999 - PP_0649 0.19 -0.2 758,087 - PP_0649 0.40 -1.3 758,087 - PP_0649 0.40 -1.2 758,189 - PP_0649 0.65 +0.2 758,189 - PP_0649 0.65 -0.7 758,306 - -0.7 758,330 + -1.7 758,331 - +0.2 758,331 - -1.1 758,331 - +0.6 758,331 - +0.6 758,340 + +0.1 758,340 + +0.1 758,341 - +0.8 758,341 - +0.1 758,358 - +1.5 758,384 + PP_0650 0.22 -1.6 758,384 + PP_0650 0.22 -0.2 758,385 - PP_0650 0.23 -1.1 758,385 - PP_0650 0.23 +0.1 758,387 + PP_0650 0.24 +0.5 758,387 + PP_0650 0.24 -0.1 758,388 - PP_0650 0.25 +1.2 758,388 - PP_0650 0.25 +0.1 758,388 - PP_0650 0.25 -0.5 758,388 - PP_0650 0.25 -2.4 758,446 - PP_0650 0.77 -0.2 758,446 - PP_0650 0.77 -0.5 758,451 + PP_0650 0.82 -1.1 758,451 + PP_0650 0.82 -0.3 758,451 + PP_0650 0.82 +0.7 758,452 - PP_0650 0.83 +0.1 758,452 - PP_0650 0.83 -2.1 758,486 + -0.9 758,486 + -2.2 758,486 + +1.1 758,514 + +0.3 758,514 + +1.4 758,515 - -1.9 758,538 + -0.3 758,539 - +0.4 758,539 - +0.3 758,539 - -1.2 758,560 - -1.1 758,562 - -0.1 758,626 - PP_0651 0.10 -0.3 758,626 - PP_0651 0.10 +1.6 758,638 + PP_0651 0.13 +0.4 758,638 + PP_0651 0.13 -0.2 758,639 - PP_0651 0.13 +0.3 758,640 + PP_0651 0.14 +0.6 758,640 + PP_0651 0.14 +1.1 758,737 + PP_0651 0.35 +0.7 758,738 - -0.5 758,757 + PP_0651 0.40 +0.3 758,757 + PP_0651 0.40 +0.8 758,758 - PP_0651 0.40 +0.8 758,758 - PP_0651 0.40 +0.9 758,763 + PP_0651 0.41 +1.2 758,764 - PP_0651 0.41 -0.0 758,881 + PP_0651 0.67 -0.4 758,881 + PP_0651 0.67 +0.4 758,881 + PP_0651 0.67 +0.2 758,883 + PP_0651 0.68 -0.4 758,883 + PP_0651 0.68 -1.9 758,883 + PP_0651 0.68 +1.2 758,884 - PP_0651 0.68 +0.1 758,913 + PP_0651 0.74 +0.8 758,913 + PP_0651 0.74 +0.6 758,913 + PP_0651 0.74 -0.6 758,913 + PP_0651 0.74 +0.3 758,913 + PP_0651 0.74 -1.4 758,914 - PP_0651 0.74 -1.8 758,914 - PP_0651 0.74 +0.3 758,914 - PP_0651 0.74 +1.8 758,914 - PP_0651 0.74 -0.2 758,914 - PP_0651 0.74 -0.2 758,948 + PP_0651 0.82 -0.1 758,948 + PP_0651 0.82 +0.5 759,011 - +1.0 759,026 + +1.3 759,026 + +0.1 759,027 - -1.3 759,042 + +2.8 759,042 + +0.4 759,047 + +0.3 759,048 - -0.3 759,048 - -0.0 759,070 - +0.1 759,070 - -0.5 759,092 + -0.8 759,092 + -0.9 759,092 + -0.3 759,092 + +0.3 759,093 - +0.2 759,093 - +0.5 759,093 - -0.6 759,093 - -0.2 759,096 + +0.0 759,096 + -0.2 759,096 + +1.3 759,097 - -0.4 759,097 - -0.3 759,118 + -1.1 759,118 + -0.0 759,119 - -0.9 759,119 - +0.3 759,202 + +1.1 759,226 + +0.5 759,276 + -0.6 759,277 - +0.1 759,288 + +1.0 759,288 + -0.5 759,288 + -1.2 759,289 - +0.1 759,294 + +0.9 759,294 + -1.7 759,295 - +0.2 759,310 + -0.7 759,310 + +0.8 759,310 + +0.3 759,310 + +1.2 759,311 - -0.2 759,313 + -0.4 759,313 + +0.1 759,374 + +0.9 759,375 - +0.4 759,375 - +0.2 759,396 + +1.0 759,419 + -0.4 759,434 - -0.5 759,607 + -1.6 759,608 - -0.2 759,608 - -0.2 759,675 + +1.7 759,675 + -0.5 759,675 + +0.1 759,675 + +0.2 759,676 - -0.5 759,676 - -1.6 759,676 - -0.9 759,676 - +0.6 759,676 - -0.5 759,676 - +1.1 759,760 - +0.3 759,810 + -0.2 759,811 - +0.0 759,811 - -1.4
Or see this region's nucleotide sequence