Experiment: P2_phage_0.5625_MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ymgA and ymgB are separated by 28 nucleotides ymgB and ymgC are separated by 112 nucleotides ymgC and b1168 are separated by 331 nucleotides b1168 and ymgF are separated by 131 nucleotides ymgF and ycgH are separated by 399 nucleotides
b1165: ymgA - hypothetical protein (NCBI), at 1,215,291 to 1,215,563
ymgA
b1166: ymgB - hypothetical protein (NCBI), at 1,215,592 to 1,215,858
ymgB
b1167: ymgC - hypothetical protein (NCBI), at 1,215,971 to 1,216,219
ymgC
b1168: b1168 - putative proteases (VIMSS), at 1,216,551 to 1,218,074
b1168
b4520: ymgF - hypothetical protein (NCBI), at 1,218,206 to 1,218,424
ymgF
b4491: ycgH - no description, at 1,218,824 to 1,221,471
ycgH
Position (kb)
1216
1217
1218
1219 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1216.034 kb on + strand, within ymgC at 1216.162 kb on + strand, within ymgC at 1216.213 kb on + strand at 1216.243 kb on + strand at 1216.274 kb on - strand at 1216.358 kb on - strand at 1216.474 kb on - strand at 1216.477 kb on - strand at 1216.479 kb on - strand at 1216.480 kb on + strand at 1216.481 kb on - strand at 1216.491 kb on - strand at 1216.491 kb on - strand at 1216.493 kb on - strand at 1216.517 kb on + strand at 1216.563 kb on - strand at 1216.566 kb on - strand at 1216.570 kb on - strand at 1216.570 kb on - strand at 1216.577 kb on - strand at 1216.652 kb on + strand at 1216.713 kb on - strand, within b1168 at 1216.714 kb on + strand, within b1168 at 1216.714 kb on + strand, within b1168 at 1216.728 kb on - strand, within b1168 at 1216.765 kb on + strand, within b1168 at 1216.772 kb on + strand, within b1168 at 1216.774 kb on - strand, within b1168 at 1216.774 kb on - strand, within b1168 at 1216.789 kb on + strand, within b1168 at 1216.795 kb on + strand, within b1168 at 1216.795 kb on + strand, within b1168 at 1216.829 kb on + strand, within b1168 at 1216.835 kb on + strand, within b1168 at 1216.838 kb on + strand, within b1168 at 1216.838 kb on - strand, within b1168 at 1216.851 kb on - strand, within b1168 at 1216.902 kb on + strand, within b1168 at 1216.902 kb on + strand, within b1168 at 1216.960 kb on - strand, within b1168 at 1217.047 kb on + strand, within b1168 at 1217.047 kb on + strand, within b1168 at 1217.086 kb on + strand, within b1168 at 1217.105 kb on + strand, within b1168 at 1217.117 kb on + strand, within b1168 at 1217.117 kb on + strand, within b1168 at 1217.199 kb on + strand, within b1168 at 1217.360 kb on - strand, within b1168 at 1217.640 kb on - strand, within b1168 at 1217.667 kb on - strand, within b1168 at 1217.690 kb on - strand, within b1168 at 1217.692 kb on - strand, within b1168 at 1217.764 kb on + strand, within b1168 at 1217.764 kb on + strand, within b1168 at 1217.767 kb on - strand, within b1168 at 1217.856 kb on - strand, within b1168 at 1217.861 kb on - strand, within b1168 at 1217.891 kb on - strand, within b1168 at 1217.891 kb on - strand, within b1168 at 1217.891 kb on - strand, within b1168 at 1218.016 kb on + strand at 1218.089 kb on + strand at 1218.096 kb on + strand at 1218.242 kb on - strand, within ymgF at 1218.284 kb on - strand, within ymgF at 1218.286 kb on - strand, within ymgF at 1218.301 kb on + strand, within ymgF at 1218.309 kb on - strand, within ymgF at 1218.311 kb on + strand, within ymgF at 1218.405 kb on + strand at 1218.414 kb on - strand at 1218.446 kb on + strand at 1218.450 kb on - strand at 1218.450 kb on - strand at 1218.454 kb on - strand at 1218.454 kb on - strand at 1218.468 kb on - strand at 1218.472 kb on - strand at 1218.478 kb on + strand at 1218.523 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI remove 1,216,034 + ymgC b1167 0.25 +1.4 1,216,162 + ymgC b1167 0.77 +1.4 1,216,213 + +1.4 1,216,243 + +3.0 1,216,274 - +1.4 1,216,358 - -0.6 1,216,474 - -0.6 1,216,477 - -0.8 1,216,479 - -0.9 1,216,480 + +1.4 1,216,481 - -0.2 1,216,491 - -0.2 1,216,491 - -0.2 1,216,493 - -1.9 1,216,517 + +0.4 1,216,563 - +0.2 1,216,566 - -1.2 1,216,570 - +0.0 1,216,570 - +1.4 1,216,577 - -1.4 1,216,652 + +2.4 1,216,713 - b1168 b1168 0.11 -2.4 1,216,714 + b1168 b1168 0.11 +1.4 1,216,714 + b1168 b1168 0.11 +0.4 1,216,728 - b1168 b1168 0.12 +1.4 1,216,765 + b1168 b1168 0.14 -0.2 1,216,772 + b1168 b1168 0.15 -1.4 1,216,774 - b1168 b1168 0.15 -0.2 1,216,774 - b1168 b1168 0.15 -0.2 1,216,789 + b1168 b1168 0.16 -0.6 1,216,795 + b1168 b1168 0.16 +0.4 1,216,795 + b1168 b1168 0.16 -0.4 1,216,829 + b1168 b1168 0.18 -1.2 1,216,835 + b1168 b1168 0.19 -1.4 1,216,838 + b1168 b1168 0.19 -2.5 1,216,838 - b1168 b1168 0.19 -1.4 1,216,851 - b1168 b1168 0.20 -2.3 1,216,902 + b1168 b1168 0.23 +0.4 1,216,902 + b1168 b1168 0.23 -0.9 1,216,960 - b1168 b1168 0.27 +1.4 1,217,047 + b1168 b1168 0.33 +4.2 1,217,047 + b1168 b1168 0.33 -0.2 1,217,086 + b1168 b1168 0.35 +1.4 1,217,105 + b1168 b1168 0.36 +0.4 1,217,117 + b1168 b1168 0.37 +1.4 1,217,117 + b1168 b1168 0.37 +1.4 1,217,199 + b1168 b1168 0.43 -1.0 1,217,360 - b1168 b1168 0.53 +1.4 1,217,640 - b1168 b1168 0.71 -0.2 1,217,667 - b1168 b1168 0.73 -0.6 1,217,690 - b1168 b1168 0.75 -1.4 1,217,692 - b1168 b1168 0.75 -1.2 1,217,764 + b1168 b1168 0.80 +1.4 1,217,764 + b1168 b1168 0.80 -0.3 1,217,767 - b1168 b1168 0.80 +1.4 1,217,856 - b1168 b1168 0.86 +1.4 1,217,861 - b1168 b1168 0.86 +1.4 1,217,891 - b1168 b1168 0.88 +1.4 1,217,891 - b1168 b1168 0.88 +0.4 1,217,891 - b1168 b1168 0.88 +1.4 1,218,016 + +1.4 1,218,089 + +1.0 1,218,096 + -0.3 1,218,242 - ymgF b4520 0.16 -1.9 1,218,284 - ymgF b4520 0.36 -0.6 1,218,286 - ymgF b4520 0.37 -1.6 1,218,301 + ymgF b4520 0.43 +1.4 1,218,309 - ymgF b4520 0.47 -1.2 1,218,311 + ymgF b4520 0.48 +0.8 1,218,405 + -3.0 1,218,414 - +2.2 1,218,446 + -0.2 1,218,450 - +1.0 1,218,450 - -0.9 1,218,454 - -0.6 1,218,454 - -0.6 1,218,468 - +1.4 1,218,472 - +1.4 1,218,478 + +0.4 1,218,523 + -0.6
Or see this region's nucleotide sequence