Strain Fitness in Pseudomonas putida KT2440 around PP_1144

Experiment: Tetrahymena sp., Mixed culture-2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1142 and PP_1143 are separated by 192 nucleotidesPP_1143 and PP_1144 overlap by 101 nucleotidesPP_1144 and PP_1145 are separated by 277 nucleotides PP_1142: PP_1142 - conserved protein of unknown function, at 1,307,911 to 1,308,288 _1142 PP_1143: PP_1143 - 3-hydroxyisobutyrate dehydrogenase family protein, at 1,308,481 to 1,309,368 _1143 PP_1144: PP_1144 - conserved membrane protein of unknown function, at 1,309,268 to 1,311,532 _1144 PP_1145: PP_1145 - RNA polymerase-associated protein RapA, at 1,311,810 to 1,314,656 _1145 Position (kb) 1309 1310 1311 1312Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1308.296 kb on + strandat 1308.363 kb on + strandat 1308.364 kb on - strandat 1308.364 kb on - strandat 1308.401 kb on + strandat 1308.401 kb on + strandat 1308.494 kb on + strandat 1308.495 kb on - strandat 1308.495 kb on - strandat 1308.677 kb on + strand, within PP_1143at 1308.677 kb on + strand, within PP_1143at 1308.677 kb on + strand, within PP_1143at 1308.975 kb on + strand, within PP_1143at 1308.975 kb on + strand, within PP_1143at 1309.046 kb on + strand, within PP_1143at 1309.047 kb on - strand, within PP_1143at 1309.047 kb on - strand, within PP_1143at 1309.047 kb on - strand, within PP_1143at 1309.199 kb on + strand, within PP_1143at 1309.199 kb on + strand, within PP_1143at 1309.200 kb on - strand, within PP_1143at 1309.200 kb on - strand, within PP_1143at 1309.315 kb on + strandat 1309.344 kb on - strandat 1309.344 kb on - strandat 1309.443 kb on + strandat 1309.443 kb on + strandat 1309.456 kb on - strandat 1309.534 kb on + strand, within PP_1144at 1309.647 kb on + strand, within PP_1144at 1309.648 kb on - strand, within PP_1144at 1309.820 kb on + strand, within PP_1144at 1310.034 kb on - strand, within PP_1144at 1310.088 kb on + strand, within PP_1144at 1310.089 kb on - strand, within PP_1144at 1310.089 kb on - strand, within PP_1144at 1310.224 kb on - strand, within PP_1144at 1310.284 kb on + strand, within PP_1144at 1310.284 kb on + strand, within PP_1144at 1310.284 kb on + strand, within PP_1144at 1310.284 kb on + strand, within PP_1144at 1310.285 kb on - strand, within PP_1144at 1310.285 kb on - strand, within PP_1144at 1310.285 kb on - strand, within PP_1144at 1310.288 kb on + strand, within PP_1144at 1310.288 kb on + strand, within PP_1144at 1310.288 kb on + strand, within PP_1144at 1310.288 kb on + strand, within PP_1144at 1310.289 kb on - strand, within PP_1144at 1310.289 kb on - strand, within PP_1144at 1310.289 kb on - strand, within PP_1144at 1310.289 kb on - strand, within PP_1144at 1310.289 kb on - strand, within PP_1144at 1310.289 kb on - strand, within PP_1144at 1310.289 kb on - strand, within PP_1144at 1310.289 kb on - strand, within PP_1144at 1310.289 kb on - strand, within PP_1144at 1310.289 kb on - strand, within PP_1144at 1310.289 kb on - strand, within PP_1144at 1310.289 kb on - strand, within PP_1144at 1310.290 kb on + strand, within PP_1144at 1310.290 kb on + strand, within PP_1144at 1310.291 kb on - strand, within PP_1144at 1310.530 kb on + strand, within PP_1144at 1310.530 kb on + strand, within PP_1144at 1310.531 kb on - strand, within PP_1144at 1310.658 kb on + strand, within PP_1144at 1310.658 kb on + strand, within PP_1144at 1310.658 kb on + strand, within PP_1144at 1310.658 kb on + strand, within PP_1144at 1310.658 kb on + strand, within PP_1144at 1310.658 kb on + strand, within PP_1144at 1310.658 kb on + strand, within PP_1144at 1310.658 kb on + strand, within PP_1144at 1310.659 kb on - strand, within PP_1144at 1310.659 kb on - strand, within PP_1144at 1310.659 kb on - strandat 1310.659 kb on - strand, within PP_1144at 1310.659 kb on - strand, within PP_1144at 1310.659 kb on - strand, within PP_1144at 1310.711 kb on + strand, within PP_1144at 1310.712 kb on - strand, within PP_1144at 1310.712 kb on - strand, within PP_1144at 1310.712 kb on - strand, within PP_1144at 1310.991 kb on + strand, within PP_1144at 1311.004 kb on - strand, within PP_1144at 1311.004 kb on - strand, within PP_1144at 1311.050 kb on + strand, within PP_1144at 1311.050 kb on + strand, within PP_1144at 1311.050 kb on + strand, within PP_1144at 1311.051 kb on - strand, within PP_1144at 1311.069 kb on + strand, within PP_1144at 1311.069 kb on + strand, within PP_1144at 1311.070 kb on - strand, within PP_1144at 1311.160 kb on - strand, within PP_1144at 1311.160 kb on - strand, within PP_1144at 1311.248 kb on + strand, within PP_1144at 1311.249 kb on - strand, within PP_1144at 1311.249 kb on - strand, within PP_1144at 1311.255 kb on + strand, within PP_1144at 1311.255 kb on + strand, within PP_1144at 1311.255 kb on + strandat 1311.255 kb on + strand, within PP_1144at 1311.256 kb on - strand, within PP_1144at 1311.256 kb on - strand, within PP_1144at 1311.256 kb on - strand, within PP_1144at 1311.311 kb on + strandat 1311.311 kb on + strandat 1311.311 kb on + strandat 1311.312 kb on - strandat 1311.312 kb on - strandat 1311.570 kb on - strandat 1311.599 kb on + strandat 1311.599 kb on + strandat 1311.599 kb on + strandat 1311.599 kb on + strandat 1311.600 kb on - strandat 1311.600 kb on - strandat 1311.601 kb on + strandat 1311.601 kb on + strandat 1311.601 kb on + strandat 1311.602 kb on - strandat 1311.602 kb on - strandat 1311.602 kb on - strandat 1311.602 kb on - strandat 1311.611 kb on + strandat 1311.612 kb on - strandat 1311.612 kb on - strandat 1311.615 kb on + strandat 1311.616 kb on - strandat 1311.630 kb on - strandat 1311.630 kb on - strandat 1311.630 kb on - strandat 1311.660 kb on + strandat 1311.683 kb on - strandat 1311.696 kb on + strandat 1311.696 kb on + strandat 1311.697 kb on - strandat 1312.049 kb on + strandat 1312.049 kb on + strandat 1312.050 kb on - strandat 1312.208 kb on - strand, within PP_1145at 1312.208 kb on - strand, within PP_1145at 1312.359 kb on + strand, within PP_1145at 1312.360 kb on - strand, within PP_1145at 1312.423 kb on + strand, within PP_1145at 1312.424 kb on - strand, within PP_1145at 1312.424 kb on - strand, within PP_1145at 1312.424 kb on - strand, within PP_1145

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Per-strain Table

Position Strand Gene LocusTag Fraction Tetrahymena sp., Mixed culture-2
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1,308,296 + -0.7
1,308,363 + -0.3
1,308,364 - +0.4
1,308,364 - -0.3
1,308,401 + -0.6
1,308,401 + +0.2
1,308,494 + -2.6
1,308,495 - -1.6
1,308,495 - -2.0
1,308,677 + PP_1143 0.22 -0.5
1,308,677 + PP_1143 0.22 -2.1
1,308,677 + PP_1143 0.22 -1.0
1,308,975 + PP_1143 0.56 +0.9
1,308,975 + PP_1143 0.56 -3.2
1,309,046 + PP_1143 0.64 +0.1
1,309,047 - PP_1143 0.64 -1.0
1,309,047 - PP_1143 0.64 +0.6
1,309,047 - PP_1143 0.64 -0.4
1,309,199 + PP_1143 0.81 +0.6
1,309,199 + PP_1143 0.81 +0.6
1,309,200 - PP_1143 0.81 -0.4
1,309,200 - PP_1143 0.81 +1.8
1,309,315 + +0.2
1,309,344 - +1.2
1,309,344 - -0.2
1,309,443 + -1.1
1,309,443 + -0.4
1,309,456 - -0.7
1,309,534 + PP_1144 0.12 +1.2
1,309,647 + PP_1144 0.17 +0.4
1,309,648 - PP_1144 0.17 +0.5
1,309,820 + PP_1144 0.24 +1.3
1,310,034 - PP_1144 0.34 +1.9
1,310,088 + PP_1144 0.36 +2.2
1,310,089 - PP_1144 0.36 +0.6
1,310,089 - PP_1144 0.36 +2.9
1,310,224 - PP_1144 0.42 +1.2
1,310,284 + PP_1144 0.45 -1.1
1,310,284 + PP_1144 0.45 +2.3
1,310,284 + PP_1144 0.45 +2.1
1,310,284 + PP_1144 0.45 -1.8
1,310,285 - PP_1144 0.45 +0.8
1,310,285 - PP_1144 0.45 +0.1
1,310,285 - PP_1144 0.45 +0.2
1,310,288 + PP_1144 0.45 +3.3
1,310,288 + PP_1144 0.45 +1.3
1,310,288 + PP_1144 0.45 -1.1
1,310,288 + PP_1144 0.45 -0.6
1,310,289 - PP_1144 0.45 -0.6
1,310,289 - PP_1144 0.45 +0.2
1,310,289 - PP_1144 0.45 -0.4
1,310,289 - PP_1144 0.45 +2.0
1,310,289 - PP_1144 0.45 -1.0
1,310,289 - PP_1144 0.45 -1.3
1,310,289 - PP_1144 0.45 +0.4
1,310,289 - PP_1144 0.45 -2.5
1,310,289 - PP_1144 0.45 +0.2
1,310,289 - PP_1144 0.45 +2.4
1,310,289 - PP_1144 0.45 +2.3
1,310,289 - PP_1144 0.45 +2.9
1,310,290 + PP_1144 0.45 +1.0
1,310,290 + PP_1144 0.45 -2.6
1,310,291 - PP_1144 0.45 -1.0
1,310,530 + PP_1144 0.56 +0.8
1,310,530 + PP_1144 0.56 -0.8
1,310,531 - PP_1144 0.56 +0.6
1,310,658 + PP_1144 0.61 -1.0
1,310,658 + PP_1144 0.61 -1.3
1,310,658 + PP_1144 0.61 +0.1
1,310,658 + PP_1144 0.61 +0.4
1,310,658 + PP_1144 0.61 +1.4
1,310,658 + PP_1144 0.61 +0.6
1,310,658 + PP_1144 0.61 +2.1
1,310,658 + PP_1144 0.61 -0.7
1,310,659 - PP_1144 0.61 +0.7
1,310,659 - PP_1144 0.61 +2.1
1,310,659 - +0.6
1,310,659 - PP_1144 0.61 +0.5
1,310,659 - PP_1144 0.61 -2.1
1,310,659 - PP_1144 0.61 +0.9
1,310,711 + PP_1144 0.64 -1.2
1,310,712 - PP_1144 0.64 +1.3
1,310,712 - PP_1144 0.64 -1.2
1,310,712 - PP_1144 0.64 -2.7
1,310,991 + PP_1144 0.76 -0.4
1,311,004 - PP_1144 0.77 +0.1
1,311,004 - PP_1144 0.77 +1.2
1,311,050 + PP_1144 0.79 -1.5
1,311,050 + PP_1144 0.79 +0.5
1,311,050 + PP_1144 0.79 +1.4
1,311,051 - PP_1144 0.79 -3.2
1,311,069 + PP_1144 0.80 +0.3
1,311,069 + PP_1144 0.80 -0.9
1,311,070 - PP_1144 0.80 -1.7
1,311,160 - PP_1144 0.84 +1.0
1,311,160 - PP_1144 0.84 -0.3
1,311,248 + PP_1144 0.87 -0.1
1,311,249 - PP_1144 0.87 +2.3
1,311,249 - PP_1144 0.87 +0.3
1,311,255 + PP_1144 0.88 -0.9
1,311,255 + PP_1144 0.88 +3.6
1,311,255 + +2.1
1,311,255 + PP_1144 0.88 -0.8
1,311,256 - PP_1144 0.88 -1.9
1,311,256 - PP_1144 0.88 +0.3
1,311,256 - PP_1144 0.88 +0.4
1,311,311 + -0.9
1,311,311 + -0.6
1,311,311 + -0.4
1,311,312 - -3.6
1,311,312 - -2.2
1,311,570 - -0.2
1,311,599 + +2.1
1,311,599 + -2.8
1,311,599 + +0.5
1,311,599 + +1.5
1,311,600 - -0.6
1,311,600 - +0.6
1,311,601 + +0.4
1,311,601 + -0.8
1,311,601 + +0.9
1,311,602 - +2.2
1,311,602 - +0.2
1,311,602 - -1.8
1,311,602 - -0.9
1,311,611 + -1.0
1,311,612 - +0.2
1,311,612 - +0.9
1,311,615 + +0.7
1,311,616 - -2.2
1,311,630 - +0.0
1,311,630 - -1.1
1,311,630 - -0.0
1,311,660 + +0.1
1,311,683 - +1.0
1,311,696 + -1.2
1,311,696 + +1.8
1,311,697 - -0.9
1,312,049 + +0.9
1,312,049 + -0.2
1,312,050 - +0.6
1,312,208 - PP_1145 0.14 +0.2
1,312,208 - PP_1145 0.14 +1.0
1,312,359 + PP_1145 0.19 +1.6
1,312,360 - PP_1145 0.19 +0.6
1,312,423 + PP_1145 0.22 +0.8
1,312,424 - PP_1145 0.22 +0.2
1,312,424 - PP_1145 0.22 -3.8
1,312,424 - PP_1145 0.22 +0.5

Or see this region's nucleotide sequence