Experiment: Stem-Atlantic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS18440 and DZA65_RS18445 are separated by 64 nucleotides DZA65_RS18445 and DZA65_RS18450 overlap by 4 nucleotides DZA65_RS18450 and DZA65_RS18455 are separated by 46 nucleotides DZA65_RS18455 and DZA65_RS18460 overlap by 8 nucleotides
DZA65_RS18440: DZA65_RS18440 - pyrroline-5-carboxylate reductase, at 3,974,861 to 3,975,682
_RS18440
DZA65_RS18445: DZA65_RS18445 - YggT family protein, at 3,975,747 to 3,976,301
_RS18445
DZA65_RS18450: DZA65_RS18450 - YggU family protein, at 3,976,298 to 3,976,588
_RS18450
DZA65_RS18455: DZA65_RS18455 - XTP/dITP diphosphatase, at 3,976,635 to 3,977,228
_RS18455
DZA65_RS18460: DZA65_RS18460 - radical SAM family heme chaperone HemW, at 3,977,221 to 3,978,360
_RS18460
Position (kb)
3976
3977 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3975.752 kb on + strand at 3975.789 kb on + strand at 3975.790 kb on - strand at 3975.790 kb on - strand at 3975.793 kb on + strand at 3975.793 kb on + strand at 3975.793 kb on + strand at 3975.793 kb on + strand at 3975.793 kb on + strand at 3975.793 kb on + strand at 3975.793 kb on + strand at 3975.793 kb on + strand at 3975.793 kb on + strand at 3975.793 kb on + strand at 3975.793 kb on + strand at 3975.793 kb on + strand at 3975.793 kb on + strand at 3975.793 kb on + strand at 3975.794 kb on - strand at 3975.794 kb on - strand at 3975.794 kb on - strand at 3975.794 kb on - strand at 3975.794 kb on - strand at 3975.794 kb on - strand at 3975.794 kb on - strand at 3975.794 kb on - strand at 3975.794 kb on - strand at 3975.843 kb on + strand, within DZA65_RS18445 at 3975.843 kb on + strand, within DZA65_RS18445 at 3975.843 kb on + strand, within DZA65_RS18445 at 3975.843 kb on + strand, within DZA65_RS18445 at 3975.843 kb on + strand, within DZA65_RS18445 at 3975.844 kb on - strand, within DZA65_RS18445 at 3975.844 kb on - strand, within DZA65_RS18445 at 3975.844 kb on - strand, within DZA65_RS18445 at 3975.844 kb on - strand, within DZA65_RS18445 at 3975.844 kb on - strand, within DZA65_RS18445 at 3975.844 kb on - strand, within DZA65_RS18445 at 3975.844 kb on - strand, within DZA65_RS18445 at 3975.844 kb on - strand, within DZA65_RS18445 at 3975.975 kb on + strand, within DZA65_RS18445 at 3975.976 kb on - strand, within DZA65_RS18445 at 3976.068 kb on + strand, within DZA65_RS18445 at 3976.114 kb on + strand, within DZA65_RS18445 at 3976.140 kb on + strand, within DZA65_RS18445 at 3976.140 kb on + strand, within DZA65_RS18445 at 3976.140 kb on + strand, within DZA65_RS18445 at 3976.140 kb on + strand, within DZA65_RS18445 at 3976.141 kb on - strand, within DZA65_RS18445 at 3976.141 kb on - strand, within DZA65_RS18445 at 3976.245 kb on + strand, within DZA65_RS18445 at 3976.245 kb on + strand, within DZA65_RS18445 at 3976.245 kb on + strand, within DZA65_RS18445 at 3976.246 kb on - strand at 3976.246 kb on - strand at 3976.246 kb on - strand at 3976.246 kb on - strand at 3976.257 kb on + strand at 3976.257 kb on + strand at 3976.257 kb on + strand at 3976.257 kb on + strand at 3976.258 kb on - strand at 3976.258 kb on - strand at 3976.258 kb on - strand at 3976.343 kb on + strand, within DZA65_RS18450 at 3976.343 kb on + strand, within DZA65_RS18450 at 3976.343 kb on + strand, within DZA65_RS18450 at 3976.344 kb on - strand, within DZA65_RS18450 at 3976.344 kb on - strand, within DZA65_RS18450 at 3976.344 kb on - strand, within DZA65_RS18450 at 3976.344 kb on - strand, within DZA65_RS18450 at 3976.344 kb on - strand, within DZA65_RS18450 at 3976.344 kb on - strand, within DZA65_RS18450 at 3976.344 kb on - strand, within DZA65_RS18450 at 3976.345 kb on + strand, within DZA65_RS18450 at 3976.345 kb on + strand, within DZA65_RS18450 at 3976.346 kb on - strand, within DZA65_RS18450 at 3976.346 kb on - strand, within DZA65_RS18450 at 3976.346 kb on - strand, within DZA65_RS18450 at 3976.346 kb on - strand, within DZA65_RS18450 at 3976.399 kb on + strand, within DZA65_RS18450 at 3976.400 kb on - strand, within DZA65_RS18450 at 3976.400 kb on - strand, within DZA65_RS18450 at 3976.400 kb on - strand, within DZA65_RS18450 at 3976.400 kb on - strand, within DZA65_RS18450 at 3976.400 kb on - strand, within DZA65_RS18450 at 3976.400 kb on - strand, within DZA65_RS18450 at 3976.425 kb on + strand, within DZA65_RS18450 at 3976.426 kb on - strand, within DZA65_RS18450 at 3976.426 kb on - strand, within DZA65_RS18450 at 3976.534 kb on + strand, within DZA65_RS18450 at 3976.534 kb on + strand, within DZA65_RS18450 at 3976.534 kb on + strand, within DZA65_RS18450 at 3976.534 kb on + strand, within DZA65_RS18450 at 3976.592 kb on + strand at 3976.593 kb on - strand at 3976.593 kb on - strand at 3976.593 kb on - strand at 3976.604 kb on - strand at 3976.604 kb on - strand at 3976.604 kb on - strand at 3976.604 kb on - strand at 3976.607 kb on + strand at 3976.652 kb on - strand at 3976.884 kb on + strand, within DZA65_RS18455 at 3976.884 kb on + strand, within DZA65_RS18455 at 3976.884 kb on + strand, within DZA65_RS18455 at 3976.885 kb on - strand, within DZA65_RS18455 at 3976.997 kb on - strand, within DZA65_RS18455 at 3977.004 kb on + strand, within DZA65_RS18455 at 3977.005 kb on - strand, within DZA65_RS18455 at 3977.005 kb on - strand, within DZA65_RS18455 at 3977.005 kb on - strand, within DZA65_RS18455 at 3977.005 kb on - strand, within DZA65_RS18455 at 3977.005 kb on - strand, within DZA65_RS18455 at 3977.101 kb on - strand, within DZA65_RS18455 at 3977.226 kb on + strand at 3977.226 kb on + strand at 3977.226 kb on + strand at 3977.226 kb on + strand at 3977.227 kb on - strand at 3977.227 kb on - strand at 3977.227 kb on - strand at 3977.227 kb on - strand at 3977.227 kb on - strand at 3977.227 kb on - strand at 3977.227 kb on - strand at 3977.241 kb on + strand at 3977.242 kb on - strand at 3977.248 kb on + strand at 3977.248 kb on + strand at 3977.248 kb on + strand at 3977.248 kb on + strand at 3977.248 kb on + strand at 3977.248 kb on + strand at 3977.248 kb on + strand at 3977.248 kb on + strand at 3977.249 kb on - strand at 3977.249 kb on - strand at 3977.249 kb on - strand at 3977.249 kb on - strand at 3977.285 kb on - strand at 3977.335 kb on + strand, within DZA65_RS18460 at 3977.335 kb on + strand, within DZA65_RS18460 at 3977.335 kb on + strand, within DZA65_RS18460 at 3977.336 kb on - strand, within DZA65_RS18460 at 3977.336 kb on - strand, within DZA65_RS18460 at 3977.336 kb on - strand, within DZA65_RS18460 at 3977.336 kb on - strand, within DZA65_RS18460 at 3977.403 kb on + strand, within DZA65_RS18460 at 3977.403 kb on + strand, within DZA65_RS18460 at 3977.403 kb on + strand, within DZA65_RS18460 at 3977.404 kb on - strand, within DZA65_RS18460 at 3977.404 kb on - strand, within DZA65_RS18460 at 3977.404 kb on - strand, within DZA65_RS18460
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Atlantic remove 3,975,752 + +0.9 3,975,789 + +0.5 3,975,790 - +1.1 3,975,790 - +0.3 3,975,793 + -0.9 3,975,793 + +0.9 3,975,793 + -0.8 3,975,793 + +0.5 3,975,793 + +1.1 3,975,793 + +1.3 3,975,793 + +0.1 3,975,793 + +2.4 3,975,793 + -2.1 3,975,793 + -2.1 3,975,793 + -1.7 3,975,793 + -0.6 3,975,793 + -0.4 3,975,793 + -0.5 3,975,794 - +0.1 3,975,794 - -1.7 3,975,794 - +0.3 3,975,794 - +1.4 3,975,794 - +0.4 3,975,794 - +0.1 3,975,794 - -1.6 3,975,794 - +0.1 3,975,794 - +1.3 3,975,843 + DZA65_RS18445 0.17 +1.1 3,975,843 + DZA65_RS18445 0.17 +0.2 3,975,843 + DZA65_RS18445 0.17 +0.5 3,975,843 + DZA65_RS18445 0.17 -0.7 3,975,843 + DZA65_RS18445 0.17 -0.5 3,975,844 - DZA65_RS18445 0.17 -0.7 3,975,844 - DZA65_RS18445 0.17 -0.7 3,975,844 - DZA65_RS18445 0.17 -2.2 3,975,844 - DZA65_RS18445 0.17 +0.3 3,975,844 - DZA65_RS18445 0.17 +2.3 3,975,844 - DZA65_RS18445 0.17 +0.5 3,975,844 - DZA65_RS18445 0.17 -1.2 3,975,844 - DZA65_RS18445 0.17 -0.9 3,975,975 + DZA65_RS18445 0.41 -0.1 3,975,976 - DZA65_RS18445 0.41 +1.1 3,976,068 + DZA65_RS18445 0.58 -1.2 3,976,114 + DZA65_RS18445 0.66 +0.2 3,976,140 + DZA65_RS18445 0.71 -0.8 3,976,140 + DZA65_RS18445 0.71 +0.5 3,976,140 + DZA65_RS18445 0.71 -1.2 3,976,140 + DZA65_RS18445 0.71 +1.1 3,976,141 - DZA65_RS18445 0.71 -1.1 3,976,141 - DZA65_RS18445 0.71 +0.6 3,976,245 + DZA65_RS18445 0.90 -0.5 3,976,245 + DZA65_RS18445 0.90 +2.7 3,976,245 + DZA65_RS18445 0.90 +0.5 3,976,246 - -0.5 3,976,246 - +0.5 3,976,246 - -1.2 3,976,246 - +1.1 3,976,257 + -1.2 3,976,257 + -0.5 3,976,257 + +0.1 3,976,257 + -1.8 3,976,258 - -1.7 3,976,258 - +0.5 3,976,258 - +0.7 3,976,343 + DZA65_RS18450 0.15 -2.1 3,976,343 + DZA65_RS18450 0.15 +2.4 3,976,343 + DZA65_RS18450 0.15 -0.5 3,976,344 - DZA65_RS18450 0.16 +0.9 3,976,344 - DZA65_RS18450 0.16 +2.8 3,976,344 - DZA65_RS18450 0.16 +0.3 3,976,344 - DZA65_RS18450 0.16 +0.6 3,976,344 - DZA65_RS18450 0.16 +0.1 3,976,344 - DZA65_RS18450 0.16 -0.9 3,976,344 - DZA65_RS18450 0.16 +1.1 3,976,345 + DZA65_RS18450 0.16 +1.3 3,976,345 + DZA65_RS18450 0.16 +1.1 3,976,346 - DZA65_RS18450 0.16 +3.1 3,976,346 - DZA65_RS18450 0.16 -1.4 3,976,346 - DZA65_RS18450 0.16 -0.9 3,976,346 - DZA65_RS18450 0.16 -3.0 3,976,399 + DZA65_RS18450 0.35 +1.4 3,976,400 - DZA65_RS18450 0.35 +0.7 3,976,400 - DZA65_RS18450 0.35 +0.4 3,976,400 - DZA65_RS18450 0.35 +0.5 3,976,400 - DZA65_RS18450 0.35 +3.3 3,976,400 - DZA65_RS18450 0.35 +2.3 3,976,400 - DZA65_RS18450 0.35 +1.8 3,976,425 + DZA65_RS18450 0.44 -0.1 3,976,426 - DZA65_RS18450 0.44 -0.2 3,976,426 - DZA65_RS18450 0.44 +1.3 3,976,534 + DZA65_RS18450 0.81 -1.2 3,976,534 + DZA65_RS18450 0.81 +0.7 3,976,534 + DZA65_RS18450 0.81 -1.9 3,976,534 + DZA65_RS18450 0.81 +1.9 3,976,592 + +0.9 3,976,593 - -1.2 3,976,593 - +0.4 3,976,593 - +0.1 3,976,604 - +1.3 3,976,604 - +0.3 3,976,604 - -0.7 3,976,604 - +0.3 3,976,607 + -2.1 3,976,652 - -1.7 3,976,884 + DZA65_RS18455 0.42 -1.9 3,976,884 + DZA65_RS18455 0.42 +0.5 3,976,884 + DZA65_RS18455 0.42 -0.7 3,976,885 - DZA65_RS18455 0.42 -1.5 3,976,997 - DZA65_RS18455 0.61 -1.6 3,977,004 + DZA65_RS18455 0.62 -1.5 3,977,005 - DZA65_RS18455 0.62 -0.5 3,977,005 - DZA65_RS18455 0.62 +1.5 3,977,005 - DZA65_RS18455 0.62 -1.2 3,977,005 - DZA65_RS18455 0.62 -2.5 3,977,005 - DZA65_RS18455 0.62 -0.6 3,977,101 - DZA65_RS18455 0.78 +2.5 3,977,226 + -2.1 3,977,226 + -0.5 3,977,226 + -0.9 3,977,226 + +0.5 3,977,227 - +0.1 3,977,227 - +0.5 3,977,227 - -1.5 3,977,227 - -1.7 3,977,227 - +0.5 3,977,227 - +0.1 3,977,227 - -0.9 3,977,241 + +0.5 3,977,242 - -0.9 3,977,248 + -2.4 3,977,248 + -1.2 3,977,248 + -0.5 3,977,248 + -1.7 3,977,248 + -0.6 3,977,248 + +1.0 3,977,248 + +0.1 3,977,248 + -0.2 3,977,249 - -1.2 3,977,249 - +0.2 3,977,249 - +0.1 3,977,249 - +1.1 3,977,285 - +1.4 3,977,335 + DZA65_RS18460 0.10 -1.5 3,977,335 + DZA65_RS18460 0.10 -1.2 3,977,335 + DZA65_RS18460 0.10 +1.1 3,977,336 - DZA65_RS18460 0.10 -2.1 3,977,336 - DZA65_RS18460 0.10 +1.5 3,977,336 - DZA65_RS18460 0.10 -0.2 3,977,336 - DZA65_RS18460 0.10 -0.9 3,977,403 + DZA65_RS18460 0.16 +0.4 3,977,403 + DZA65_RS18460 0.16 -1.2 3,977,403 + DZA65_RS18460 0.16 +0.4 3,977,404 - DZA65_RS18460 0.16 -1.0 3,977,404 - DZA65_RS18460 0.16 -2.2 3,977,404 - DZA65_RS18460 0.16 -1.7
Or see this region's nucleotide sequence