Experiment: Stem-Atlantic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS12340 and DZA65_RS12345 are separated by 141 nucleotides DZA65_RS12345 and DZA65_RS12350 are separated by 12 nucleotides DZA65_RS12350 and DZA65_RS12355 overlap by 4 nucleotides DZA65_RS12355 and DZA65_RS12360 are separated by 1 nucleotides
DZA65_RS12340: DZA65_RS12340 - amidohydrolase, at 2,635,029 to 2,636,231
_RS12340
DZA65_RS12345: DZA65_RS12345 - SDR family oxidoreductase, at 2,636,373 to 2,637,152
_RS12345
DZA65_RS12350: DZA65_RS12350 - ABC transporter ATP-binding protein, at 2,637,165 to 2,638,862
_RS12350
DZA65_RS12355: DZA65_RS12355 - ABC transporter permease, at 2,638,859 to 2,639,728
_RS12355
DZA65_RS12360: DZA65_RS12360 - ABC transporter permease, at 2,639,730 to 2,640,716
_RS12360
Position (kb)
2637
2638
2639 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2636.168 kb on + strand at 2636.199 kb on + strand at 2636.200 kb on - strand at 2636.200 kb on - strand at 2636.200 kb on - strand at 2636.200 kb on - strand at 2636.200 kb on - strand at 2636.221 kb on + strand at 2636.221 kb on + strand at 2636.221 kb on + strand at 2636.221 kb on + strand at 2636.221 kb on + strand at 2636.222 kb on - strand at 2636.334 kb on + strand at 2636.536 kb on + strand, within DZA65_RS12345 at 2636.537 kb on - strand, within DZA65_RS12345 at 2636.612 kb on + strand, within DZA65_RS12345 at 2636.613 kb on - strand, within DZA65_RS12345 at 2636.613 kb on - strand, within DZA65_RS12345 at 2636.638 kb on - strand at 2636.641 kb on - strand, within DZA65_RS12345 at 2636.954 kb on + strand, within DZA65_RS12345 at 2636.954 kb on + strand, within DZA65_RS12345 at 2636.954 kb on + strand, within DZA65_RS12345 at 2636.954 kb on + strand, within DZA65_RS12345 at 2636.955 kb on - strand, within DZA65_RS12345 at 2637.117 kb on + strand at 2637.117 kb on + strand at 2637.117 kb on + strand at 2637.118 kb on - strand at 2637.118 kb on - strand at 2637.166 kb on + strand at 2637.166 kb on + strand at 2637.166 kb on + strand at 2637.167 kb on - strand at 2637.167 kb on - strand at 2637.167 kb on - strand at 2637.242 kb on + strand at 2637.242 kb on + strand at 2637.242 kb on + strand at 2637.242 kb on + strand at 2637.242 kb on + strand at 2637.243 kb on - strand at 2637.244 kb on + strand at 2637.244 kb on + strand at 2637.244 kb on + strand at 2637.245 kb on - strand at 2637.245 kb on - strand at 2637.334 kb on + strand at 2637.334 kb on + strand at 2637.334 kb on + strand at 2637.394 kb on - strand, within DZA65_RS12350 at 2637.630 kb on + strand, within DZA65_RS12350 at 2637.630 kb on + strand, within DZA65_RS12350 at 2637.630 kb on + strand, within DZA65_RS12350 at 2637.630 kb on + strand, within DZA65_RS12350 at 2637.630 kb on + strand, within DZA65_RS12350 at 2637.630 kb on + strand, within DZA65_RS12350 at 2637.630 kb on + strand, within DZA65_RS12350 at 2637.631 kb on - strand, within DZA65_RS12350 at 2637.631 kb on - strand, within DZA65_RS12350 at 2637.631 kb on - strand, within DZA65_RS12350 at 2637.631 kb on - strand, within DZA65_RS12350 at 2637.631 kb on - strand, within DZA65_RS12350 at 2637.755 kb on + strand, within DZA65_RS12350 at 2637.755 kb on + strand, within DZA65_RS12350 at 2637.756 kb on - strand, within DZA65_RS12350 at 2637.759 kb on - strand, within DZA65_RS12350 at 2637.759 kb on - strand, within DZA65_RS12350 at 2637.996 kb on - strand, within DZA65_RS12350 at 2637.996 kb on - strand, within DZA65_RS12350 at 2638.127 kb on + strand, within DZA65_RS12350 at 2638.129 kb on + strand, within DZA65_RS12350 at 2638.129 kb on + strand, within DZA65_RS12350 at 2638.129 kb on + strand, within DZA65_RS12350 at 2638.129 kb on + strand, within DZA65_RS12350 at 2638.130 kb on - strand, within DZA65_RS12350 at 2638.833 kb on + strand at 2638.862 kb on + strand at 2638.862 kb on + strand at 2638.862 kb on + strand at 2638.862 kb on + strand at 2638.862 kb on + strand at 2638.863 kb on - strand at 2638.863 kb on - strand at 2638.863 kb on - strand at 2638.863 kb on - strand at 2638.863 kb on - strand at 2638.863 kb on - strand at 2638.863 kb on - strand at 2638.863 kb on - strand at 2639.212 kb on + strand, within DZA65_RS12355 at 2639.333 kb on - strand, within DZA65_RS12355 at 2639.333 kb on - strand, within DZA65_RS12355 at 2639.657 kb on - strand at 2639.783 kb on + strand at 2639.783 kb on + strand at 2639.784 kb on - strand at 2639.854 kb on - strand, within DZA65_RS12360
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Atlantic remove 2,636,168 + -0.9 2,636,199 + -1.7 2,636,200 - +1.9 2,636,200 - -1.8 2,636,200 - -0.9 2,636,200 - -0.6 2,636,200 - +0.7 2,636,221 + -0.3 2,636,221 + +0.4 2,636,221 + +1.8 2,636,221 + +1.3 2,636,221 + -0.1 2,636,222 - +1.0 2,636,334 + -0.6 2,636,536 + DZA65_RS12345 0.21 -0.9 2,636,537 - DZA65_RS12345 0.21 -0.0 2,636,612 + DZA65_RS12345 0.31 +0.3 2,636,613 - DZA65_RS12345 0.31 -0.2 2,636,613 - DZA65_RS12345 0.31 -0.1 2,636,638 - +0.3 2,636,641 - DZA65_RS12345 0.34 +0.3 2,636,954 + DZA65_RS12345 0.74 -0.9 2,636,954 + DZA65_RS12345 0.74 +1.3 2,636,954 + DZA65_RS12345 0.74 -1.7 2,636,954 + DZA65_RS12345 0.74 -1.1 2,636,955 - DZA65_RS12345 0.75 -1.7 2,637,117 + +2.0 2,637,117 + -0.9 2,637,117 + -2.5 2,637,118 - +0.3 2,637,118 - -0.7 2,637,166 + -0.2 2,637,166 + +2.3 2,637,166 + -1.3 2,637,167 - -1.1 2,637,167 - -2.7 2,637,167 - -2.6 2,637,242 + -2.3 2,637,242 + -1.7 2,637,242 + -0.2 2,637,242 + +0.7 2,637,242 + -0.6 2,637,243 - -0.5 2,637,244 + -0.5 2,637,244 + +0.7 2,637,244 + +1.3 2,637,245 - +0.1 2,637,245 - +0.3 2,637,334 + +2.3 2,637,334 + -0.3 2,637,334 + -0.9 2,637,394 - DZA65_RS12350 0.13 +0.6 2,637,630 + DZA65_RS12350 0.27 -1.3 2,637,630 + DZA65_RS12350 0.27 -0.6 2,637,630 + DZA65_RS12350 0.27 +0.7 2,637,630 + DZA65_RS12350 0.27 -0.6 2,637,630 + DZA65_RS12350 0.27 +0.7 2,637,630 + DZA65_RS12350 0.27 +0.7 2,637,630 + DZA65_RS12350 0.27 -0.0 2,637,631 - DZA65_RS12350 0.27 +1.5 2,637,631 - DZA65_RS12350 0.27 -1.1 2,637,631 - DZA65_RS12350 0.27 -2.1 2,637,631 - DZA65_RS12350 0.27 +1.3 2,637,631 - DZA65_RS12350 0.27 -1.3 2,637,755 + DZA65_RS12350 0.35 -1.3 2,637,755 + DZA65_RS12350 0.35 +1.4 2,637,756 - DZA65_RS12350 0.35 -2.2 2,637,759 - DZA65_RS12350 0.35 -0.8 2,637,759 - DZA65_RS12350 0.35 +0.9 2,637,996 - DZA65_RS12350 0.49 -0.2 2,637,996 - DZA65_RS12350 0.49 +0.2 2,638,127 + DZA65_RS12350 0.57 +1.3 2,638,129 + DZA65_RS12350 0.57 +0.7 2,638,129 + DZA65_RS12350 0.57 +0.8 2,638,129 + DZA65_RS12350 0.57 +0.7 2,638,129 + DZA65_RS12350 0.57 -2.7 2,638,130 - DZA65_RS12350 0.57 +0.1 2,638,833 + -0.1 2,638,862 + -1.3 2,638,862 + -0.6 2,638,862 + +1.6 2,638,862 + -1.3 2,638,862 + +2.5 2,638,863 - -1.3 2,638,863 - -0.7 2,638,863 - -1.3 2,638,863 - +1.3 2,638,863 - +1.7 2,638,863 - +0.3 2,638,863 - -0.2 2,638,863 - -0.3 2,639,212 + DZA65_RS12355 0.41 +0.7 2,639,333 - DZA65_RS12355 0.54 +1.2 2,639,333 - DZA65_RS12355 0.54 +0.4 2,639,657 - -0.1 2,639,783 + -1.0 2,639,783 + +0.6 2,639,784 - -1.3 2,639,854 - DZA65_RS12360 0.13 -0.1
Or see this region's nucleotide sequence