Experiment: Stem-Atlantic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS13115 and DZA65_RS13120 are separated by 130 nucleotides DZA65_RS13120 and DZA65_RS13125 are separated by 109 nucleotides DZA65_RS13125 and DZA65_RS13130 are separated by 331 nucleotides
DZA65_RS13115: DZA65_RS13115 - lactoylglutathione lyase, at 2,812,820 to 2,813,227
_RS13115
DZA65_RS13120: DZA65_RS13120 - ribonuclease T, at 2,813,358 to 2,814,044
_RS13120
DZA65_RS13125: DZA65_RS13125 - Grx4 family monothiol glutaredoxin, at 2,814,154 to 2,814,504
_RS13125
DZA65_RS13130: DZA65_RS13130 - C40 family peptidase, at 2,814,836 to 2,815,822
_RS13130
Position (kb)
2814
2815 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2813.372 kb on + strand at 2813.372 kb on + strand at 2813.409 kb on + strand at 2813.409 kb on + strand at 2813.562 kb on - strand, within DZA65_RS13120 at 2813.634 kb on + strand, within DZA65_RS13120 at 2813.718 kb on - strand, within DZA65_RS13120 at 2813.910 kb on + strand, within DZA65_RS13120 at 2813.910 kb on + strand, within DZA65_RS13120 at 2813.911 kb on - strand, within DZA65_RS13120 at 2813.911 kb on - strand, within DZA65_RS13120 at 2813.911 kb on - strand, within DZA65_RS13120 at 2814.043 kb on - strand at 2814.043 kb on - strand at 2814.043 kb on - strand at 2814.043 kb on - strand at 2814.074 kb on + strand at 2814.075 kb on - strand at 2814.085 kb on - strand at 2814.102 kb on + strand at 2814.142 kb on + strand at 2814.155 kb on + strand at 2814.156 kb on - strand at 2814.230 kb on + strand, within DZA65_RS13125 at 2814.230 kb on + strand, within DZA65_RS13125 at 2814.231 kb on - strand, within DZA65_RS13125 at 2814.308 kb on + strand, within DZA65_RS13125 at 2814.349 kb on - strand, within DZA65_RS13125 at 2814.349 kb on - strand, within DZA65_RS13125 at 2814.443 kb on + strand, within DZA65_RS13125 at 2814.443 kb on + strand, within DZA65_RS13125 at 2814.444 kb on - strand, within DZA65_RS13125 at 2814.444 kb on - strand, within DZA65_RS13125 at 2814.444 kb on - strand, within DZA65_RS13125 at 2814.444 kb on - strand, within DZA65_RS13125 at 2814.508 kb on - strand at 2814.508 kb on - strand at 2814.598 kb on - strand at 2814.602 kb on + strand at 2814.603 kb on - strand at 2814.603 kb on - strand at 2814.631 kb on + strand at 2814.696 kb on - strand at 2814.696 kb on - strand at 2814.700 kb on - strand at 2814.700 kb on - strand at 2814.716 kb on + strand at 2814.719 kb on + strand at 2814.744 kb on - strand at 2814.744 kb on - strand at 2814.744 kb on - strand at 2814.790 kb on - strand at 2814.833 kb on - strand at 2814.847 kb on + strand at 2814.847 kb on + strand at 2814.848 kb on - strand at 2814.848 kb on - strand at 2814.901 kb on + strand at 2814.902 kb on - strand at 2814.910 kb on + strand at 2814.955 kb on + strand, within DZA65_RS13130 at 2814.961 kb on + strand, within DZA65_RS13130 at 2814.962 kb on - strand, within DZA65_RS13130 at 2815.085 kb on - strand, within DZA65_RS13130 at 2815.109 kb on - strand, within DZA65_RS13130 at 2815.141 kb on + strand, within DZA65_RS13130 at 2815.141 kb on + strand, within DZA65_RS13130 at 2815.141 kb on + strand, within DZA65_RS13130 at 2815.141 kb on + strand, within DZA65_RS13130 at 2815.141 kb on + strand, within DZA65_RS13130 at 2815.142 kb on - strand, within DZA65_RS13130 at 2815.142 kb on - strand, within DZA65_RS13130 at 2815.199 kb on - strand, within DZA65_RS13130 at 2815.376 kb on - strand, within DZA65_RS13130 at 2815.426 kb on + strand, within DZA65_RS13130 at 2815.472 kb on + strand, within DZA65_RS13130
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Atlantic remove 2,813,372 + -2.0 2,813,372 + -2.0 2,813,409 + -3.5 2,813,409 + +0.2 2,813,562 - DZA65_RS13120 0.30 -0.5 2,813,634 + DZA65_RS13120 0.40 -0.9 2,813,718 - DZA65_RS13120 0.52 +0.9 2,813,910 + DZA65_RS13120 0.80 -1.7 2,813,910 + DZA65_RS13120 0.80 -0.7 2,813,911 - DZA65_RS13120 0.80 -0.6 2,813,911 - DZA65_RS13120 0.80 -1.3 2,813,911 - DZA65_RS13120 0.80 -1.1 2,814,043 - +0.4 2,814,043 - +1.1 2,814,043 - -2.0 2,814,043 - -0.5 2,814,074 + -0.9 2,814,075 - +0.2 2,814,085 - +0.1 2,814,102 + -1.1 2,814,142 + +1.3 2,814,155 + -2.3 2,814,156 - +1.3 2,814,230 + DZA65_RS13125 0.22 -0.9 2,814,230 + DZA65_RS13125 0.22 -1.7 2,814,231 - DZA65_RS13125 0.22 +0.0 2,814,308 + DZA65_RS13125 0.44 -1.6 2,814,349 - DZA65_RS13125 0.56 -0.1 2,814,349 - DZA65_RS13125 0.56 +1.3 2,814,443 + DZA65_RS13125 0.82 -0.7 2,814,443 + DZA65_RS13125 0.82 -0.0 2,814,444 - DZA65_RS13125 0.83 -1.3 2,814,444 - DZA65_RS13125 0.83 -0.7 2,814,444 - DZA65_RS13125 0.83 +0.4 2,814,444 - DZA65_RS13125 0.83 -1.3 2,814,508 - -2.5 2,814,508 - -2.1 2,814,598 - +0.0 2,814,602 + -2.5 2,814,603 - +0.7 2,814,603 - -0.1 2,814,631 + -0.7 2,814,696 - +0.8 2,814,696 - +1.4 2,814,700 - +1.6 2,814,700 - -1.2 2,814,716 + -1.7 2,814,719 + -0.3 2,814,744 - -0.6 2,814,744 - -0.0 2,814,744 - -2.0 2,814,790 - -2.3 2,814,833 - +0.1 2,814,847 + -0.3 2,814,847 + -0.7 2,814,848 - -1.1 2,814,848 - +0.3 2,814,901 + -1.7 2,814,902 - +0.9 2,814,910 + +0.2 2,814,955 + DZA65_RS13130 0.12 +0.9 2,814,961 + DZA65_RS13130 0.13 +0.2 2,814,962 - DZA65_RS13130 0.13 -2.3 2,815,085 - DZA65_RS13130 0.25 +1.1 2,815,109 - DZA65_RS13130 0.28 +1.1 2,815,141 + DZA65_RS13130 0.31 -0.4 2,815,141 + DZA65_RS13130 0.31 -0.2 2,815,141 + DZA65_RS13130 0.31 -1.3 2,815,141 + DZA65_RS13130 0.31 -0.7 2,815,141 + DZA65_RS13130 0.31 +0.7 2,815,142 - DZA65_RS13130 0.31 +1.3 2,815,142 - DZA65_RS13130 0.31 +0.8 2,815,199 - DZA65_RS13130 0.37 +0.3 2,815,376 - DZA65_RS13130 0.55 -1.5 2,815,426 + DZA65_RS13130 0.60 +0.7 2,815,472 + DZA65_RS13130 0.64 +0.1
Or see this region's nucleotide sequence