Experiment: Stem-Atlantic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS03495 and DZA65_RS03500 are separated by 166 nucleotides DZA65_RS03500 and DZA65_RS03505 are separated by 9 nucleotides
DZA65_RS03495: DZA65_RS03495 - single-stranded-DNA-specific exonuclease RecJ, at 737,736 to 739,466
_RS03495
DZA65_RS03500: DZA65_RS03500 - peptide chain release factor 2, at 739,633 to 740,731
_RS03500
DZA65_RS03505: DZA65_RS03505 - lysine--tRNA ligase, at 740,741 to 742,258
_RS03505
Position (kb) 739
740
741 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 738.813 kb on + strand, within DZA65_RS03495 at 738.813 kb on + strand, within DZA65_RS03495 at 738.813 kb on + strand, within DZA65_RS03495 at 738.813 kb on + strand, within DZA65_RS03495 at 738.813 kb on + strand, within DZA65_RS03495 at 738.814 kb on - strand, within DZA65_RS03495 at 738.814 kb on - strand, within DZA65_RS03495 at 738.814 kb on - strand, within DZA65_RS03495 at 738.814 kb on - strand, within DZA65_RS03495 at 738.981 kb on - strand, within DZA65_RS03495 at 738.981 kb on - strand, within DZA65_RS03495 at 739.392 kb on + strand at 739.393 kb on - strand at 739.432 kb on + strand at 739.433 kb on - strand at 739.433 kb on - strand at 739.433 kb on - strand at 739.465 kb on - strand at 739.465 kb on - strand at 739.478 kb on + strand at 739.479 kb on - strand at 739.486 kb on - strand at 739.486 kb on - strand at 739.551 kb on + strand at 739.553 kb on + strand at 739.553 kb on + strand at 739.588 kb on + strand at 739.588 kb on + strand at 739.588 kb on + strand at 739.588 kb on + strand at 739.588 kb on + strand at 739.618 kb on + strand at 739.618 kb on + strand at 739.618 kb on + strand at 739.618 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Atlantic remove 738,813 + DZA65_RS03495 0.62 +0.0 738,813 + DZA65_RS03495 0.62 -3.5 738,813 + DZA65_RS03495 0.62 -1.4 738,813 + DZA65_RS03495 0.62 -0.5 738,813 + DZA65_RS03495 0.62 -1.0 738,814 - DZA65_RS03495 0.62 -1.0 738,814 - DZA65_RS03495 0.62 +0.6 738,814 - DZA65_RS03495 0.62 -0.4 738,814 - DZA65_RS03495 0.62 -0.4 738,981 - DZA65_RS03495 0.72 -2.0 738,981 - DZA65_RS03495 0.72 +0.2 739,392 + +0.4 739,393 - -1.0 739,432 + +0.1 739,433 - -1.0 739,433 - +0.1 739,433 - -1.0 739,465 - -2.4 739,465 - +2.2 739,478 + +1.4 739,479 - -2.8 739,486 - +0.0 739,486 - -0.7 739,551 + -0.7 739,553 + -1.2 739,553 + +0.1 739,588 + -0.2 739,588 + +0.6 739,588 + +2.2 739,588 + +0.3 739,588 + +1.0 739,618 + -1.7 739,618 + -2.6 739,618 + +2.2 739,618 + -0.4
Or see this region's nucleotide sequence