Experiment: Stem-Atlantic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS00965 and DZA65_RS00970 are separated by 80 nucleotides DZA65_RS00970 and DZA65_RS00975 are separated by 3 nucleotides DZA65_RS00975 and DZA65_RS00980 are separated by 3 nucleotides DZA65_RS00980 and DZA65_RS00985 are separated by 113 nucleotides
DZA65_RS00965: DZA65_RS00965 - ubiquinone biosynthesis regulatory protein kinase UbiB, at 221,054 to 222,691
_RS00965
DZA65_RS00970: DZA65_RS00970 - twin-arginine translocase subunit TatA, at 222,772 to 223,026
_RS00970
DZA65_RS00975: DZA65_RS00975 - Sec-independent protein translocase subunit TatB, at 223,030 to 223,602
_RS00975
DZA65_RS00980: DZA65_RS00980 - Sec-independent protein translocase subunit TatC, at 223,606 to 224,373
_RS00980
DZA65_RS00985: DZA65_RS00985 - 3'-5' ssDNA/RNA exonuclease TatD, at 224,487 to 225,281
_RS00985
Position (kb) 223
224 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 222.702 kb on + strand at 222.702 kb on + strand at 222.703 kb on - strand at 222.755 kb on - strand at 222.762 kb on + strand at 222.762 kb on + strand at 222.762 kb on + strand at 222.762 kb on + strand at 222.762 kb on + strand at 222.763 kb on - strand at 222.772 kb on - strand at 222.780 kb on + strand at 222.781 kb on - strand at 222.783 kb on + strand at 222.783 kb on + strand at 222.783 kb on + strand at 222.783 kb on + strand at 222.783 kb on + strand at 222.783 kb on + strand at 222.784 kb on - strand at 222.784 kb on - strand at 222.784 kb on - strand at 222.784 kb on - strand at 222.786 kb on + strand at 222.786 kb on + strand at 222.786 kb on + strand at 222.787 kb on - strand at 222.818 kb on + strand, within DZA65_RS00970 at 222.818 kb on + strand, within DZA65_RS00970 at 222.819 kb on - strand, within DZA65_RS00970 at 222.819 kb on - strand, within DZA65_RS00970 at 222.819 kb on - strand, within DZA65_RS00970 at 222.821 kb on + strand, within DZA65_RS00970 at 222.821 kb on + strand at 222.822 kb on - strand, within DZA65_RS00970 at 222.822 kb on - strand, within DZA65_RS00970 at 222.935 kb on + strand, within DZA65_RS00970 at 222.936 kb on - strand, within DZA65_RS00970 at 222.936 kb on - strand, within DZA65_RS00970 at 223.011 kb on + strand at 223.012 kb on - strand at 223.024 kb on + strand at 223.025 kb on - strand at 223.025 kb on - strand at 223.025 kb on - strand at 223.025 kb on - strand at 223.473 kb on + strand, within DZA65_RS00975 at 223.473 kb on + strand, within DZA65_RS00975 at 223.474 kb on - strand, within DZA65_RS00975 at 223.568 kb on + strand at 223.568 kb on + strand at 223.569 kb on - strand at 223.569 kb on - strand at 223.569 kb on - strand at 223.600 kb on + strand at 223.600 kb on + strand at 223.605 kb on + strand at 223.605 kb on + strand at 223.605 kb on + strand at 223.605 kb on + strand at 223.605 kb on + strand at 223.606 kb on - strand at 223.606 kb on - strand at 223.671 kb on + strand at 223.671 kb on + strand at 223.671 kb on + strand at 223.672 kb on - strand at 223.672 kb on - strand at 223.677 kb on + strand at 223.677 kb on + strand at 223.677 kb on + strand at 223.678 kb on - strand at 223.711 kb on + strand, within DZA65_RS00980 at 223.712 kb on - strand, within DZA65_RS00980 at 223.729 kb on + strand, within DZA65_RS00980 at 223.729 kb on + strand at 223.729 kb on + strand, within DZA65_RS00980 at 223.780 kb on - strand, within DZA65_RS00980 at 223.870 kb on + strand, within DZA65_RS00980 at 223.870 kb on + strand, within DZA65_RS00980 at 223.871 kb on - strand, within DZA65_RS00980 at 223.871 kb on - strand, within DZA65_RS00980 at 223.871 kb on - strand, within DZA65_RS00980 at 223.871 kb on - strand, within DZA65_RS00980 at 223.871 kb on - strand, within DZA65_RS00980 at 223.903 kb on + strand, within DZA65_RS00980 at 223.903 kb on + strand, within DZA65_RS00980 at 223.903 kb on + strand, within DZA65_RS00980 at 223.903 kb on + strand, within DZA65_RS00980 at 223.904 kb on - strand, within DZA65_RS00980 at 223.960 kb on + strand, within DZA65_RS00980 at 223.960 kb on + strand, within DZA65_RS00980 at 223.960 kb on + strand, within DZA65_RS00980 at 223.960 kb on + strand, within DZA65_RS00980 at 223.960 kb on + strand, within DZA65_RS00980 at 223.960 kb on + strand, within DZA65_RS00980 at 223.960 kb on + strand, within DZA65_RS00980 at 223.960 kb on + strand, within DZA65_RS00980 at 223.960 kb on + strand, within DZA65_RS00980 at 223.960 kb on + strand, within DZA65_RS00980 at 223.960 kb on + strand, within DZA65_RS00980 at 223.960 kb on + strand, within DZA65_RS00980 at 223.960 kb on + strand, within DZA65_RS00980 at 223.961 kb on - strand, within DZA65_RS00980 at 223.961 kb on - strand, within DZA65_RS00980 at 223.961 kb on - strand, within DZA65_RS00980 at 223.961 kb on - strand, within DZA65_RS00980 at 223.961 kb on - strand, within DZA65_RS00980 at 223.961 kb on - strand, within DZA65_RS00980 at 223.962 kb on + strand, within DZA65_RS00980 at 223.981 kb on + strand, within DZA65_RS00980 at 224.007 kb on + strand, within DZA65_RS00980 at 224.061 kb on + strand, within DZA65_RS00980 at 224.061 kb on + strand, within DZA65_RS00980 at 224.061 kb on + strand, within DZA65_RS00980 at 224.062 kb on - strand, within DZA65_RS00980 at 224.062 kb on - strand, within DZA65_RS00980 at 224.062 kb on - strand at 224.062 kb on - strand, within DZA65_RS00980 at 224.062 kb on - strand, within DZA65_RS00980 at 224.062 kb on - strand, within DZA65_RS00980 at 224.065 kb on + strand, within DZA65_RS00980 at 224.065 kb on + strand, within DZA65_RS00980 at 224.065 kb on + strand, within DZA65_RS00980 at 224.065 kb on + strand, within DZA65_RS00980 at 224.065 kb on + strand, within DZA65_RS00980 at 224.065 kb on + strand, within DZA65_RS00980 at 224.066 kb on - strand, within DZA65_RS00980 at 224.066 kb on - strand, within DZA65_RS00980 at 224.066 kb on - strand, within DZA65_RS00980 at 224.066 kb on - strand, within DZA65_RS00980 at 224.066 kb on - strand, within DZA65_RS00980 at 224.066 kb on - strand, within DZA65_RS00980 at 224.189 kb on - strand, within DZA65_RS00980 at 224.272 kb on + strand, within DZA65_RS00980 at 224.272 kb on + strand, within DZA65_RS00980 at 224.272 kb on + strand, within DZA65_RS00980 at 224.272 kb on + strand, within DZA65_RS00980 at 224.272 kb on + strand, within DZA65_RS00980 at 224.273 kb on - strand, within DZA65_RS00980 at 224.273 kb on - strand at 224.273 kb on - strand, within DZA65_RS00980 at 224.273 kb on - strand, within DZA65_RS00980 at 224.276 kb on + strand at 224.276 kb on + strand, within DZA65_RS00980 at 224.276 kb on + strand, within DZA65_RS00980 at 224.276 kb on + strand, within DZA65_RS00980 at 224.276 kb on + strand, within DZA65_RS00980 at 224.276 kb on + strand, within DZA65_RS00980 at 224.276 kb on + strand, within DZA65_RS00980 at 224.276 kb on + strand, within DZA65_RS00980 at 224.276 kb on + strand, within DZA65_RS00980 at 224.276 kb on + strand, within DZA65_RS00980 at 224.277 kb on - strand, within DZA65_RS00980 at 224.311 kb on + strand at 224.311 kb on + strand at 224.312 kb on - strand at 224.312 kb on - strand at 224.405 kb on + strand at 224.405 kb on + strand at 224.406 kb on - strand at 224.406 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Atlantic remove 222,702 + +0.7 222,702 + +1.9 222,703 - -0.7 222,755 - -1.1 222,762 + +2.4 222,762 + -2.3 222,762 + -1.9 222,762 + +0.9 222,762 + +1.9 222,763 - -1.4 222,772 - -1.7 222,780 + -0.4 222,781 - -2.0 222,783 + -0.6 222,783 + +1.7 222,783 + -2.1 222,783 + +0.7 222,783 + -0.5 222,783 + -1.4 222,784 - -0.4 222,784 - -2.1 222,784 - -1.6 222,784 - -2.1 222,786 + +0.2 222,786 + -1.4 222,786 + +0.8 222,787 - -0.5 222,818 + DZA65_RS00970 0.18 -0.3 222,818 + DZA65_RS00970 0.18 -0.3 222,819 - DZA65_RS00970 0.18 +0.2 222,819 - DZA65_RS00970 0.18 -1.4 222,819 - DZA65_RS00970 0.18 -1.8 222,821 + DZA65_RS00970 0.19 +2.0 222,821 + -2.4 222,822 - DZA65_RS00970 0.20 +0.1 222,822 - DZA65_RS00970 0.20 -1.8 222,935 + DZA65_RS00970 0.64 +0.9 222,936 - DZA65_RS00970 0.64 -3.5 222,936 - DZA65_RS00970 0.64 -0.4 223,011 + -0.4 223,012 - +0.3 223,024 + -0.2 223,025 - -1.6 223,025 - -1.7 223,025 - +1.9 223,025 - -1.7 223,473 + DZA65_RS00975 0.77 -1.1 223,473 + DZA65_RS00975 0.77 +0.2 223,474 - DZA65_RS00975 0.77 -0.7 223,568 + +0.1 223,568 + +1.9 223,569 - -1.3 223,569 - -1.1 223,569 - -2.3 223,600 + -1.1 223,600 + -0.4 223,605 + -1.1 223,605 + +0.2 223,605 + -0.7 223,605 + -2.0 223,605 + -0.7 223,606 - -1.0 223,606 - -2.5 223,671 + -2.3 223,671 + +0.3 223,671 + -0.8 223,672 - -0.7 223,672 - -0.7 223,677 + -1.6 223,677 + -0.4 223,677 + -1.7 223,678 - -2.4 223,711 + DZA65_RS00980 0.14 +0.2 223,712 - DZA65_RS00980 0.14 -2.9 223,729 + DZA65_RS00980 0.16 -1.6 223,729 + -0.7 223,729 + DZA65_RS00980 0.16 -1.4 223,780 - DZA65_RS00980 0.23 -2.7 223,870 + DZA65_RS00980 0.34 -1.1 223,870 + DZA65_RS00980 0.34 -2.4 223,871 - DZA65_RS00980 0.35 -0.9 223,871 - DZA65_RS00980 0.35 -2.1 223,871 - DZA65_RS00980 0.35 -0.7 223,871 - DZA65_RS00980 0.35 -3.1 223,871 - DZA65_RS00980 0.35 -0.4 223,903 + DZA65_RS00980 0.39 -2.6 223,903 + DZA65_RS00980 0.39 -3.9 223,903 + DZA65_RS00980 0.39 +0.3 223,903 + DZA65_RS00980 0.39 -0.7 223,904 - DZA65_RS00980 0.39 -3.4 223,960 + DZA65_RS00980 0.46 -1.7 223,960 + DZA65_RS00980 0.46 +2.3 223,960 + DZA65_RS00980 0.46 -1.7 223,960 + DZA65_RS00980 0.46 -3.4 223,960 + DZA65_RS00980 0.46 +0.9 223,960 + DZA65_RS00980 0.46 +0.9 223,960 + DZA65_RS00980 0.46 -2.3 223,960 + DZA65_RS00980 0.46 -1.0 223,960 + DZA65_RS00980 0.46 -1.1 223,960 + DZA65_RS00980 0.46 -0.4 223,960 + DZA65_RS00980 0.46 -0.7 223,960 + DZA65_RS00980 0.46 -2.9 223,960 + DZA65_RS00980 0.46 -0.4 223,961 - DZA65_RS00980 0.46 -0.8 223,961 - DZA65_RS00980 0.46 -3.3 223,961 - DZA65_RS00980 0.46 -1.5 223,961 - DZA65_RS00980 0.46 -2.3 223,961 - DZA65_RS00980 0.46 -1.3 223,961 - DZA65_RS00980 0.46 -0.1 223,962 + DZA65_RS00980 0.46 -3.0 223,981 + DZA65_RS00980 0.49 -2.2 224,007 + DZA65_RS00980 0.52 -2.4 224,061 + DZA65_RS00980 0.59 -1.2 224,061 + DZA65_RS00980 0.59 +0.9 224,061 + DZA65_RS00980 0.59 +0.9 224,062 - DZA65_RS00980 0.59 -2.4 224,062 - DZA65_RS00980 0.59 -1.7 224,062 - -0.3 224,062 - DZA65_RS00980 0.59 -1.1 224,062 - DZA65_RS00980 0.59 -1.3 224,062 - DZA65_RS00980 0.59 -2.6 224,065 + DZA65_RS00980 0.60 -0.7 224,065 + DZA65_RS00980 0.60 -0.1 224,065 + DZA65_RS00980 0.60 -1.1 224,065 + DZA65_RS00980 0.60 -0.2 224,065 + DZA65_RS00980 0.60 -0.1 224,065 + DZA65_RS00980 0.60 +0.7 224,066 - DZA65_RS00980 0.60 +0.3 224,066 - DZA65_RS00980 0.60 -2.7 224,066 - DZA65_RS00980 0.60 -0.9 224,066 - DZA65_RS00980 0.60 -1.8 224,066 - DZA65_RS00980 0.60 -1.4 224,066 - DZA65_RS00980 0.60 -2.7 224,189 - DZA65_RS00980 0.76 -0.1 224,272 + DZA65_RS00980 0.87 -0.1 224,272 + DZA65_RS00980 0.87 -1.9 224,272 + DZA65_RS00980 0.87 -1.1 224,272 + DZA65_RS00980 0.87 -2.4 224,272 + DZA65_RS00980 0.87 -0.4 224,273 - DZA65_RS00980 0.87 -2.1 224,273 - -0.1 224,273 - DZA65_RS00980 0.87 -0.1 224,273 - DZA65_RS00980 0.87 -1.1 224,276 + -2.4 224,276 + DZA65_RS00980 0.87 -1.4 224,276 + DZA65_RS00980 0.87 -0.1 224,276 + DZA65_RS00980 0.87 +0.3 224,276 + DZA65_RS00980 0.87 +0.3 224,276 + DZA65_RS00980 0.87 -0.2 224,276 + DZA65_RS00980 0.87 -1.7 224,276 + DZA65_RS00980 0.87 -2.4 224,276 + DZA65_RS00980 0.87 -3.0 224,276 + DZA65_RS00980 0.87 +0.9 224,277 - DZA65_RS00980 0.87 -3.3 224,311 + +0.7 224,311 + +0.3 224,312 - +0.7 224,312 - -0.4 224,405 + -0.6 224,405 + +1.2 224,406 - +0.4 224,406 - +0.9
Or see this region's nucleotide sequence