Experiment: Stem-Atlantic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS10775 and DZA65_RS10780 are separated by 266 nucleotides DZA65_RS10780 and DZA65_RS10785 are separated by 84 nucleotides
DZA65_RS10775: DZA65_RS10775 - signal peptide peptidase SppA, at 2,279,493 to 2,281,343
_RS10775
DZA65_RS10780: DZA65_RS10780 - NAD(P)H nitroreductase, at 2,281,610 to 2,282,161
_RS10780
DZA65_RS10785: DZA65_RS10785 - DNA topoisomerase III, at 2,282,246 to 2,284,171
_RS10785
Position (kb)
2281
2282
2283 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2280.635 kb on - strand, within DZA65_RS10775 at 2280.665 kb on + strand at 2280.666 kb on - strand, within DZA65_RS10775 at 2280.727 kb on + strand, within DZA65_RS10775 at 2280.728 kb on - strand, within DZA65_RS10775 at 2280.728 kb on - strand, within DZA65_RS10775 at 2280.728 kb on - strand, within DZA65_RS10775 at 2280.784 kb on + strand, within DZA65_RS10775 at 2280.785 kb on - strand, within DZA65_RS10775 at 2280.785 kb on - strand, within DZA65_RS10775 at 2280.787 kb on + strand, within DZA65_RS10775 at 2280.787 kb on + strand, within DZA65_RS10775 at 2280.787 kb on + strand, within DZA65_RS10775 at 2280.788 kb on - strand, within DZA65_RS10775 at 2280.868 kb on + strand, within DZA65_RS10775 at 2280.868 kb on + strand, within DZA65_RS10775 at 2280.869 kb on - strand, within DZA65_RS10775 at 2280.869 kb on - strand, within DZA65_RS10775 at 2280.869 kb on - strand, within DZA65_RS10775 at 2280.872 kb on - strand, within DZA65_RS10775 at 2280.872 kb on - strand, within DZA65_RS10775 at 2280.872 kb on - strand, within DZA65_RS10775 at 2280.872 kb on - strand, within DZA65_RS10775 at 2280.872 kb on - strand, within DZA65_RS10775 at 2280.872 kb on - strand, within DZA65_RS10775 at 2280.952 kb on + strand, within DZA65_RS10775 at 2280.952 kb on + strand, within DZA65_RS10775 at 2280.952 kb on + strand, within DZA65_RS10775 at 2280.952 kb on + strand, within DZA65_RS10775 at 2280.953 kb on - strand, within DZA65_RS10775 at 2280.953 kb on - strand, within DZA65_RS10775 at 2280.953 kb on - strand, within DZA65_RS10775 at 2280.953 kb on - strand, within DZA65_RS10775 at 2280.953 kb on - strand, within DZA65_RS10775 at 2280.953 kb on - strand, within DZA65_RS10775 at 2280.953 kb on - strand, within DZA65_RS10775 at 2280.953 kb on - strand, within DZA65_RS10775 at 2281.053 kb on - strand, within DZA65_RS10775 at 2281.161 kb on + strand at 2281.173 kb on + strand at 2281.209 kb on + strand at 2281.209 kb on + strand at 2281.220 kb on - strand at 2281.220 kb on - strand at 2281.220 kb on - strand at 2281.220 kb on - strand at 2281.220 kb on - strand at 2281.220 kb on - strand at 2281.220 kb on - strand at 2281.269 kb on - strand at 2281.312 kb on - strand at 2281.372 kb on + strand at 2281.417 kb on + strand at 2281.476 kb on - strand at 2281.476 kb on - strand at 2281.486 kb on + strand at 2281.486 kb on + strand at 2281.486 kb on + strand at 2281.486 kb on + strand at 2281.490 kb on + strand at 2281.490 kb on + strand at 2281.491 kb on - strand at 2281.491 kb on - strand at 2281.491 kb on - strand at 2281.491 kb on - strand at 2281.539 kb on + strand at 2281.540 kb on - strand at 2281.548 kb on + strand at 2281.571 kb on + strand at 2281.571 kb on + strand at 2281.571 kb on + strand at 2281.572 kb on - strand at 2281.572 kb on - strand at 2281.629 kb on + strand at 2281.693 kb on + strand, within DZA65_RS10780 at 2281.909 kb on + strand, within DZA65_RS10780 at 2281.909 kb on + strand, within DZA65_RS10780 at 2281.910 kb on - strand, within DZA65_RS10780 at 2281.935 kb on + strand, within DZA65_RS10780 at 2281.996 kb on + strand, within DZA65_RS10780 at 2282.085 kb on - strand, within DZA65_RS10780 at 2282.085 kb on - strand, within DZA65_RS10780 at 2282.085 kb on - strand, within DZA65_RS10780 at 2282.134 kb on + strand at 2282.135 kb on - strand at 2282.153 kb on + strand at 2282.153 kb on + strand at 2282.153 kb on + strand at 2282.153 kb on + strand at 2282.154 kb on - strand at 2282.154 kb on - strand at 2282.154 kb on - strand at 2282.159 kb on + strand at 2282.159 kb on + strand at 2282.160 kb on - strand at 2282.221 kb on + strand at 2282.405 kb on + strand at 2282.405 kb on + strand at 2282.405 kb on + strand at 2282.406 kb on - strand at 2282.574 kb on + strand, within DZA65_RS10785 at 2282.574 kb on + strand, within DZA65_RS10785 at 2282.598 kb on - strand, within DZA65_RS10785 at 2282.760 kb on - strand, within DZA65_RS10785 at 2282.760 kb on - strand, within DZA65_RS10785 at 2282.760 kb on - strand, within DZA65_RS10785 at 2282.783 kb on + strand, within DZA65_RS10785 at 2282.784 kb on - strand, within DZA65_RS10785 at 2282.784 kb on - strand, within DZA65_RS10785 at 2282.784 kb on - strand, within DZA65_RS10785 at 2282.784 kb on - strand, within DZA65_RS10785 at 2282.784 kb on - strand, within DZA65_RS10785 at 2282.784 kb on - strand, within DZA65_RS10785 at 2282.785 kb on + strand, within DZA65_RS10785 at 2282.785 kb on + strand, within DZA65_RS10785 at 2282.786 kb on - strand, within DZA65_RS10785 at 2282.786 kb on - strand, within DZA65_RS10785 at 2282.786 kb on - strand, within DZA65_RS10785 at 2282.786 kb on - strand, within DZA65_RS10785 at 2282.786 kb on - strand, within DZA65_RS10785 at 2282.786 kb on - strand, within DZA65_RS10785 at 2282.906 kb on + strand, within DZA65_RS10785 at 2282.907 kb on - strand, within DZA65_RS10785 at 2282.990 kb on + strand at 2283.080 kb on + strand, within DZA65_RS10785 at 2283.080 kb on + strand, within DZA65_RS10785 at 2283.081 kb on - strand, within DZA65_RS10785 at 2283.081 kb on - strand, within DZA65_RS10785 at 2283.081 kb on - strand, within DZA65_RS10785 at 2283.081 kb on - strand, within DZA65_RS10785 at 2283.081 kb on - strand, within DZA65_RS10785 at 2283.082 kb on + strand, within DZA65_RS10785 at 2283.082 kb on + strand, within DZA65_RS10785 at 2283.083 kb on - strand, within DZA65_RS10785 at 2283.083 kb on - strand, within DZA65_RS10785 at 2283.083 kb on - strand, within DZA65_RS10785 at 2283.088 kb on + strand, within DZA65_RS10785
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Atlantic remove 2,280,635 - DZA65_RS10775 0.62 +1.5 2,280,665 + -0.5 2,280,666 - DZA65_RS10775 0.63 -2.3 2,280,727 + DZA65_RS10775 0.67 +1.5 2,280,728 - DZA65_RS10775 0.67 -0.8 2,280,728 - DZA65_RS10775 0.67 -0.7 2,280,728 - DZA65_RS10775 0.67 +0.5 2,280,784 + DZA65_RS10775 0.70 -2.3 2,280,785 - DZA65_RS10775 0.70 -0.1 2,280,785 - DZA65_RS10775 0.70 -0.2 2,280,787 + DZA65_RS10775 0.70 -2.1 2,280,787 + DZA65_RS10775 0.70 -2.1 2,280,787 + DZA65_RS10775 0.70 -0.2 2,280,788 - DZA65_RS10775 0.70 +1.5 2,280,868 + DZA65_RS10775 0.74 +1.5 2,280,868 + DZA65_RS10775 0.74 -0.8 2,280,869 - DZA65_RS10775 0.74 -0.1 2,280,869 - DZA65_RS10775 0.74 -1.1 2,280,869 - DZA65_RS10775 0.74 -0.6 2,280,872 - DZA65_RS10775 0.75 +0.5 2,280,872 - DZA65_RS10775 0.75 +0.3 2,280,872 - DZA65_RS10775 0.75 -1.8 2,280,872 - DZA65_RS10775 0.75 -0.2 2,280,872 - DZA65_RS10775 0.75 +0.3 2,280,872 - DZA65_RS10775 0.75 -0.1 2,280,952 + DZA65_RS10775 0.79 +0.5 2,280,952 + DZA65_RS10775 0.79 +0.0 2,280,952 + DZA65_RS10775 0.79 +0.7 2,280,952 + DZA65_RS10775 0.79 -2.1 2,280,953 - DZA65_RS10775 0.79 +0.9 2,280,953 - DZA65_RS10775 0.79 +0.1 2,280,953 - DZA65_RS10775 0.79 +0.3 2,280,953 - DZA65_RS10775 0.79 +0.1 2,280,953 - DZA65_RS10775 0.79 -1.1 2,280,953 - DZA65_RS10775 0.79 +1.5 2,280,953 - DZA65_RS10775 0.79 +1.5 2,280,953 - DZA65_RS10775 0.79 +0.1 2,281,053 - DZA65_RS10775 0.84 -0.3 2,281,161 + -1.8 2,281,173 + -1.5 2,281,209 + -0.3 2,281,209 + -0.5 2,281,220 - +0.9 2,281,220 - -0.5 2,281,220 - -0.3 2,281,220 - -1.1 2,281,220 - -2.3 2,281,220 - -0.1 2,281,220 - -2.8 2,281,269 - +0.9 2,281,312 - -0.6 2,281,372 + +1.1 2,281,417 + -0.1 2,281,476 - +0.5 2,281,476 - +1.8 2,281,486 + -1.5 2,281,486 + -0.2 2,281,486 + -0.3 2,281,486 + +1.5 2,281,490 + +0.5 2,281,490 + +0.9 2,281,491 - -1.0 2,281,491 - -1.8 2,281,491 - -0.7 2,281,491 - +2.1 2,281,539 + -1.2 2,281,540 - +1.5 2,281,548 + +0.0 2,281,571 + +0.0 2,281,571 + -0.4 2,281,571 + +1.3 2,281,572 - -1.8 2,281,572 - +1.3 2,281,629 + -0.4 2,281,693 + DZA65_RS10780 0.15 +0.5 2,281,909 + DZA65_RS10780 0.54 +0.7 2,281,909 + DZA65_RS10780 0.54 -0.6 2,281,910 - DZA65_RS10780 0.54 +0.5 2,281,935 + DZA65_RS10780 0.59 -0.3 2,281,996 + DZA65_RS10780 0.70 +0.5 2,282,085 - DZA65_RS10780 0.86 +0.5 2,282,085 - DZA65_RS10780 0.86 +0.5 2,282,085 - DZA65_RS10780 0.86 -0.1 2,282,134 + -0.5 2,282,135 - -0.8 2,282,153 + +0.1 2,282,153 + +0.9 2,282,153 + +0.1 2,282,153 + -0.8 2,282,154 - -0.5 2,282,154 - +0.7 2,282,154 - -0.3 2,282,159 + +0.8 2,282,159 + -1.5 2,282,160 - +0.5 2,282,221 + +0.1 2,282,405 + +3.1 2,282,405 + +0.5 2,282,405 + +1.5 2,282,406 - -0.1 2,282,574 + DZA65_RS10785 0.17 +0.0 2,282,574 + DZA65_RS10785 0.17 +0.0 2,282,598 - DZA65_RS10785 0.18 -1.5 2,282,760 - DZA65_RS10785 0.27 +2.1 2,282,760 - DZA65_RS10785 0.27 -0.5 2,282,760 - DZA65_RS10785 0.27 +0.9 2,282,783 + DZA65_RS10785 0.28 -2.6 2,282,784 - DZA65_RS10785 0.28 -0.6 2,282,784 - DZA65_RS10785 0.28 -0.5 2,282,784 - DZA65_RS10785 0.28 -1.1 2,282,784 - DZA65_RS10785 0.28 -2.2 2,282,784 - DZA65_RS10785 0.28 +1.3 2,282,784 - DZA65_RS10785 0.28 +0.8 2,282,785 + DZA65_RS10785 0.28 -0.1 2,282,785 + DZA65_RS10785 0.28 +0.5 2,282,786 - DZA65_RS10785 0.28 +0.5 2,282,786 - DZA65_RS10785 0.28 -0.5 2,282,786 - DZA65_RS10785 0.28 -0.5 2,282,786 - DZA65_RS10785 0.28 -0.7 2,282,786 - DZA65_RS10785 0.28 -0.8 2,282,786 - DZA65_RS10785 0.28 +1.3 2,282,906 + DZA65_RS10785 0.34 -1.1 2,282,907 - DZA65_RS10785 0.34 +1.0 2,282,990 + -2.3 2,283,080 + DZA65_RS10785 0.43 +0.5 2,283,080 + DZA65_RS10785 0.43 -1.1 2,283,081 - DZA65_RS10785 0.43 -0.6 2,283,081 - DZA65_RS10785 0.43 -0.7 2,283,081 - DZA65_RS10785 0.43 -0.0 2,283,081 - DZA65_RS10785 0.43 +0.8 2,283,081 - DZA65_RS10785 0.43 -1.6 2,283,082 + DZA65_RS10785 0.43 +1.0 2,283,082 + DZA65_RS10785 0.43 -0.7 2,283,083 - DZA65_RS10785 0.43 +1.2 2,283,083 - DZA65_RS10785 0.43 -2.1 2,283,083 - DZA65_RS10785 0.43 -1.1 2,283,088 + DZA65_RS10785 0.44 -0.7
Or see this region's nucleotide sequence