Experiment: Stem-Atlantic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS03185 and DZA65_RS03190 are separated by 157 nucleotides DZA65_RS03190 and DZA65_RS03195 are separated by 47 nucleotides DZA65_RS03195 and DZA65_RS03200 are separated by 105 nucleotides DZA65_RS03200 and DZA65_RS03205 are separated by 8 nucleotides
DZA65_RS03185: DZA65_RS03185 - ribosome assembly RNA-binding protein YhbY, at 676,342 to 676,635
_RS03185
DZA65_RS03190: DZA65_RS03190 - 23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE, at 676,793 to 677,422
_RS03190
DZA65_RS03195: DZA65_RS03195 - ATP-dependent zinc metalloprotease FtsH, at 677,470 to 679,422
_RS03195
DZA65_RS03200: DZA65_RS03200 - dihydropteroate synthase, at 679,528 to 680,361
_RS03200
DZA65_RS03205: DZA65_RS03205 - phosphoglucosamine mutase, at 680,370 to 681,707
_RS03205
Position (kb) 677
678
679
680 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 676.535 kb on - strand, within DZA65_RS03185 at 676.535 kb on - strand, within DZA65_RS03185 at 676.535 kb on - strand, within DZA65_RS03185 at 676.623 kb on + strand at 676.676 kb on + strand at 676.693 kb on + strand at 676.693 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.758 kb on + strand at 676.758 kb on + strand at 676.758 kb on + strand at 676.758 kb on + strand at 676.764 kb on + strand at 676.764 kb on + strand at 676.779 kb on + strand at 677.420 kb on + strand at 677.420 kb on + strand at 677.420 kb on + strand at 677.423 kb on + strand at 679.311 kb on + strand at 679.311 kb on + strand at 679.311 kb on + strand at 679.389 kb on + strand at 679.389 kb on + strand at 679.392 kb on + strand at 679.392 kb on + strand at 679.392 kb on + strand at 679.392 kb on + strand at 679.392 kb on + strand at 679.393 kb on - strand at 679.393 kb on - strand at 679.393 kb on - strand at 679.393 kb on - strand at 679.420 kb on + strand at 679.420 kb on + strand at 679.420 kb on + strand at 679.420 kb on + strand at 679.420 kb on + strand at 679.420 kb on + strand at 679.420 kb on + strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.427 kb on - strand at 679.427 kb on - strand at 679.427 kb on - strand at 679.427 kb on - strand at 679.427 kb on - strand at 679.427 kb on - strand at 679.448 kb on + strand at 679.448 kb on + strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.484 kb on - strand at 679.484 kb on - strand at 679.515 kb on + strand at 679.516 kb on - strand at 679.516 kb on - strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.325 kb on - strand at 680.325 kb on - strand at 680.325 kb on - strand at 680.330 kb on + strand at 680.330 kb on + strand at 680.330 kb on + strand at 680.346 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Atlantic remove 676,535 - DZA65_RS03185 0.66 -1.2 676,535 - DZA65_RS03185 0.66 -1.3 676,535 - DZA65_RS03185 0.66 -2.8 676,623 + -0.3 676,676 + +0.8 676,693 + +0.2 676,693 + -1.8 676,754 + -1.4 676,754 + -1.0 676,754 + -1.3 676,754 + -2.0 676,754 + +0.0 676,754 + +0.6 676,758 + +0.7 676,758 + +1.1 676,758 + -2.0 676,758 + +2.0 676,764 + +0.3 676,764 + +0.1 676,779 + +0.8 677,420 + -1.3 677,420 + -0.3 677,420 + -2.1 677,423 + -1.0 679,311 + +1.5 679,311 + -1.1 679,311 + -2.0 679,389 + +1.0 679,389 + -1.0 679,392 + -1.3 679,392 + +0.8 679,392 + +1.6 679,392 + -1.3 679,392 + -2.2 679,393 - -0.6 679,393 - -1.3 679,393 - -1.0 679,393 - -0.4 679,420 + -2.2 679,420 + -1.1 679,420 + -0.6 679,420 + -1.6 679,420 + -1.1 679,420 + -2.3 679,420 + -0.2 679,421 - -2.3 679,421 - -2.1 679,421 - -1.3 679,421 - -2.2 679,421 - +0.0 679,421 - -2.0 679,421 - -0.9 679,421 - -1.4 679,421 - -1.3 679,421 - -1.7 679,427 - -1.2 679,427 - -0.7 679,427 - -2.6 679,427 - -0.6 679,427 - -1.3 679,427 - -2.4 679,448 + +2.0 679,448 + -0.8 679,449 - +0.5 679,449 - -1.3 679,449 - -1.1 679,449 - +0.2 679,449 - -2.0 679,449 - -1.6 679,449 - -1.7 679,449 - +0.5 679,449 - -0.8 679,484 - +1.0 679,484 - +1.0 679,515 + +0.0 679,516 - -2.6 679,516 - -0.6 680,324 + +0.0 680,324 + -1.8 680,324 + -2.3 680,324 + -0.6 680,324 + -2.3 680,324 + -2.0 680,324 + -3.2 680,324 + -1.8 680,324 + -2.2 680,325 - -4.7 680,325 - +1.0 680,325 - -1.8 680,330 + +0.5 680,330 + -0.6 680,330 + -2.0 680,346 - -1.0
Or see this region's nucleotide sequence