Experiment: Stem-Atlantic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS03175 and DZA65_RS03180 are separated by 264 nucleotides DZA65_RS03180 and DZA65_RS03185 are separated by 123 nucleotides DZA65_RS03185 and DZA65_RS03190 are separated by 157 nucleotides DZA65_RS03190 and DZA65_RS03195 are separated by 47 nucleotides
DZA65_RS03175: DZA65_RS03175 - serine-type D-Ala-D-Ala carboxypeptidase, at 674,044 to 675,477
_RS03175
DZA65_RS03180: DZA65_RS03180 - transcription elongation factor GreA, at 675,742 to 676,218
_RS03180
DZA65_RS03185: DZA65_RS03185 - ribosome assembly RNA-binding protein YhbY, at 676,342 to 676,635
_RS03185
DZA65_RS03190: DZA65_RS03190 - 23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE, at 676,793 to 677,422
_RS03190
DZA65_RS03195: DZA65_RS03195 - ATP-dependent zinc metalloprotease FtsH, at 677,470 to 679,422
_RS03195
Position (kb)
676
677 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 675.392 kb on + strand at 675.393 kb on - strand at 675.401 kb on + strand at 675.401 kb on + strand at 675.402 kb on - strand at 675.402 kb on - strand at 675.402 kb on - strand at 675.478 kb on - strand at 675.478 kb on - strand at 675.492 kb on - strand at 675.492 kb on - strand at 675.512 kb on + strand at 675.512 kb on + strand at 675.513 kb on - strand at 675.513 kb on - strand at 675.513 kb on - strand at 675.513 kb on - strand at 675.515 kb on + strand at 675.516 kb on - strand at 675.537 kb on + strand at 675.538 kb on - strand at 675.626 kb on + strand at 675.626 kb on + strand at 675.627 kb on - strand at 675.636 kb on + strand at 675.684 kb on + strand at 675.684 kb on + strand at 675.684 kb on + strand at 675.684 kb on + strand at 675.684 kb on + strand at 675.684 kb on + strand at 675.685 kb on - strand at 675.685 kb on - strand at 675.685 kb on - strand at 675.685 kb on - strand at 675.685 kb on - strand at 675.727 kb on - strand at 675.732 kb on + strand at 675.732 kb on + strand at 675.732 kb on + strand at 675.732 kb on + strand at 675.732 kb on + strand at 675.742 kb on - strand at 675.783 kb on - strand at 675.828 kb on + strand, within DZA65_RS03180 at 675.828 kb on + strand, within DZA65_RS03180 at 675.883 kb on + strand, within DZA65_RS03180 at 675.883 kb on + strand, within DZA65_RS03180 at 675.883 kb on + strand, within DZA65_RS03180 at 675.884 kb on - strand, within DZA65_RS03180 at 675.884 kb on - strand, within DZA65_RS03180 at 675.964 kb on - strand, within DZA65_RS03180 at 676.012 kb on - strand, within DZA65_RS03180 at 676.054 kb on + strand, within DZA65_RS03180 at 676.054 kb on + strand, within DZA65_RS03180 at 676.055 kb on - strand, within DZA65_RS03180 at 676.055 kb on - strand, within DZA65_RS03180 at 676.059 kb on + strand, within DZA65_RS03180 at 676.098 kb on + strand, within DZA65_RS03180 at 676.098 kb on + strand, within DZA65_RS03180 at 676.098 kb on + strand, within DZA65_RS03180 at 676.099 kb on - strand, within DZA65_RS03180 at 676.099 kb on - strand, within DZA65_RS03180 at 676.099 kb on - strand, within DZA65_RS03180 at 676.099 kb on - strand, within DZA65_RS03180 at 676.099 kb on - strand, within DZA65_RS03180 at 676.099 kb on - strand, within DZA65_RS03180 at 676.133 kb on + strand, within DZA65_RS03180 at 676.184 kb on + strand at 676.184 kb on + strand at 676.185 kb on - strand at 676.185 kb on - strand at 676.210 kb on + strand at 676.211 kb on - strand at 676.211 kb on - strand at 676.251 kb on + strand at 676.251 kb on + strand at 676.279 kb on + strand at 676.383 kb on - strand, within DZA65_RS03185 at 676.535 kb on - strand, within DZA65_RS03185 at 676.535 kb on - strand, within DZA65_RS03185 at 676.535 kb on - strand, within DZA65_RS03185 at 676.623 kb on + strand at 676.676 kb on + strand at 676.693 kb on + strand at 676.693 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.758 kb on + strand at 676.758 kb on + strand at 676.758 kb on + strand at 676.758 kb on + strand at 676.764 kb on + strand at 676.764 kb on + strand at 676.779 kb on + strand at 677.420 kb on + strand at 677.420 kb on + strand at 677.420 kb on + strand at 677.423 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Atlantic remove 675,392 + +1.8 675,393 - +0.0 675,401 + +0.5 675,401 + +0.6 675,402 - -2.5 675,402 - -0.3 675,402 - +1.9 675,478 - +0.3 675,478 - +0.3 675,492 - -0.3 675,492 - +1.0 675,512 + +0.7 675,512 + +1.8 675,513 - -1.0 675,513 - +3.3 675,513 - +1.0 675,513 - +1.0 675,515 + +0.7 675,516 - -1.0 675,537 + -1.0 675,538 - +0.6 675,626 + -0.9 675,626 + +1.0 675,627 - +0.8 675,636 + -0.9 675,684 + +0.4 675,684 + +0.0 675,684 + +1.8 675,684 + -1.0 675,684 + +0.3 675,684 + +0.2 675,685 - +1.6 675,685 - +0.8 675,685 - +1.2 675,685 - -2.7 675,685 - +1.0 675,727 - -1.0 675,732 + -0.2 675,732 + +0.0 675,732 + +0.9 675,732 + +1.8 675,732 + +2.0 675,742 - +0.0 675,783 - +0.7 675,828 + DZA65_RS03180 0.18 -1.0 675,828 + DZA65_RS03180 0.18 +0.0 675,883 + DZA65_RS03180 0.30 -0.6 675,883 + DZA65_RS03180 0.30 -4.0 675,883 + DZA65_RS03180 0.30 -1.6 675,884 - DZA65_RS03180 0.30 -1.1 675,884 - DZA65_RS03180 0.30 +0.4 675,964 - DZA65_RS03180 0.47 -2.8 676,012 - DZA65_RS03180 0.57 -2.4 676,054 + DZA65_RS03180 0.65 +0.2 676,054 + DZA65_RS03180 0.65 -3.6 676,055 - DZA65_RS03180 0.66 -0.6 676,055 - DZA65_RS03180 0.66 -1.6 676,059 + DZA65_RS03180 0.66 -1.6 676,098 + DZA65_RS03180 0.75 -1.4 676,098 + DZA65_RS03180 0.75 -1.9 676,098 + DZA65_RS03180 0.75 -0.3 676,099 - DZA65_RS03180 0.75 -1.6 676,099 - DZA65_RS03180 0.75 -2.1 676,099 - DZA65_RS03180 0.75 -1.1 676,099 - DZA65_RS03180 0.75 -1.6 676,099 - DZA65_RS03180 0.75 +0.0 676,099 - DZA65_RS03180 0.75 -3.6 676,133 + DZA65_RS03180 0.82 -3.2 676,184 + +0.1 676,184 + -1.0 676,185 - -1.3 676,185 - -2.2 676,210 + +0.0 676,211 - -1.0 676,211 - +0.6 676,251 + -1.1 676,251 + -1.0 676,279 + +0.0 676,383 - DZA65_RS03185 0.14 -1.7 676,535 - DZA65_RS03185 0.66 -1.2 676,535 - DZA65_RS03185 0.66 -1.3 676,535 - DZA65_RS03185 0.66 -2.8 676,623 + -0.3 676,676 + +0.8 676,693 + +0.2 676,693 + -1.8 676,754 + -1.4 676,754 + -1.0 676,754 + -1.3 676,754 + -2.0 676,754 + +0.0 676,754 + +0.6 676,758 + +0.7 676,758 + +1.1 676,758 + -2.0 676,758 + +2.0 676,764 + +0.3 676,764 + +0.1 676,779 + +0.8 677,420 + -1.3 677,420 + -0.3 677,420 + -2.1 677,423 + -1.0
Or see this region's nucleotide sequence