Experiment: Stem-Atlantic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS01660 and DZA65_RS01665 are separated by 135 nucleotides DZA65_RS01665 and DZA65_RS01670 are separated by 10 nucleotides DZA65_RS01670 and DZA65_RS01675 are separated by 73 nucleotides DZA65_RS01675 and DZA65_RS01680 overlap by 43 nucleotides
DZA65_RS01660: DZA65_RS01660 - alginate lyase family protein, at 361,775 to 363,076
_RS01660
DZA65_RS01665: DZA65_RS01665 - divisome-associated lipoprotein YraP, at 363,212 to 363,787
_RS01665
DZA65_RS01670: DZA65_RS01670 - DnaA initiator-associating protein DiaA, at 363,798 to 364,388
_RS01670
DZA65_RS01675: DZA65_RS01675 - YraN family protein, at 364,462 to 364,857
_RS01675
DZA65_RS01680: DZA65_RS01680 - penicillin-binding protein activator, at 364,815 to 366,866
_RS01680
Position (kb) 363
364
365 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 362.822 kb on + strand, within DZA65_RS01660 at 362.850 kb on - strand, within DZA65_RS01660 at 362.851 kb on + strand, within DZA65_RS01660 at 362.851 kb on + strand, within DZA65_RS01660 at 362.851 kb on + strand, within DZA65_RS01660 at 362.851 kb on + strand, within DZA65_RS01660 at 362.851 kb on + strand, within DZA65_RS01660 at 362.851 kb on + strand, within DZA65_RS01660 at 362.852 kb on - strand, within DZA65_RS01660 at 362.852 kb on - strand, within DZA65_RS01660 at 362.857 kb on + strand, within DZA65_RS01660 at 362.858 kb on - strand, within DZA65_RS01660 at 362.858 kb on - strand, within DZA65_RS01660 at 362.858 kb on - strand, within DZA65_RS01660 at 362.956 kb on + strand at 362.957 kb on - strand at 363.078 kb on + strand at 363.079 kb on - strand at 363.083 kb on - strand at 363.094 kb on + strand at 363.095 kb on - strand at 363.099 kb on - strand at 363.110 kb on + strand at 363.197 kb on - strand at 363.197 kb on - strand at 363.213 kb on + strand at 363.214 kb on - strand at 363.214 kb on - strand at 363.214 kb on - strand at 363.549 kb on - strand, within DZA65_RS01665 at 363.778 kb on - strand at 363.778 kb on - strand at 363.778 kb on - strand at 363.778 kb on - strand at 363.778 kb on - strand at 363.778 kb on - strand at 363.799 kb on + strand at 363.800 kb on - strand at 363.800 kb on - strand at 363.827 kb on + strand at 363.828 kb on - strand at 363.834 kb on + strand at 363.834 kb on + strand at 363.834 kb on + strand at 363.834 kb on + strand at 363.834 kb on + strand at 363.834 kb on + strand at 363.835 kb on - strand at 363.863 kb on + strand, within DZA65_RS01670 at 363.863 kb on + strand, within DZA65_RS01670 at 363.864 kb on - strand, within DZA65_RS01670 at 363.864 kb on - strand, within DZA65_RS01670 at 363.864 kb on - strand, within DZA65_RS01670 at 363.864 kb on - strand, within DZA65_RS01670 at 363.864 kb on - strand, within DZA65_RS01670 at 363.864 kb on - strand, within DZA65_RS01670 at 363.955 kb on + strand, within DZA65_RS01670 at 364.093 kb on + strand, within DZA65_RS01670 at 364.142 kb on + strand, within DZA65_RS01670 at 364.151 kb on + strand, within DZA65_RS01670 at 364.151 kb on + strand, within DZA65_RS01670 at 364.151 kb on + strand, within DZA65_RS01670 at 364.152 kb on - strand, within DZA65_RS01670 at 364.152 kb on - strand, within DZA65_RS01670 at 364.172 kb on + strand, within DZA65_RS01670 at 364.172 kb on + strand, within DZA65_RS01670 at 364.202 kb on + strand, within DZA65_RS01670 at 364.203 kb on - strand, within DZA65_RS01670 at 364.203 kb on - strand, within DZA65_RS01670 at 364.203 kb on - strand, within DZA65_RS01670 at 364.373 kb on + strand at 364.373 kb on + strand at 364.374 kb on - strand at 364.374 kb on - strand at 364.374 kb on - strand at 364.407 kb on + strand at 364.408 kb on - strand at 364.439 kb on + strand at 364.439 kb on + strand at 364.439 kb on + strand at 364.439 kb on + strand at 364.440 kb on - strand at 364.440 kb on - strand at 364.451 kb on + strand at 364.452 kb on - strand at 364.452 kb on - strand at 364.464 kb on - strand at 364.620 kb on + strand, within DZA65_RS01675 at 364.620 kb on + strand, within DZA65_RS01675 at 364.651 kb on - strand, within DZA65_RS01675 at 364.698 kb on + strand, within DZA65_RS01675 at 364.699 kb on - strand, within DZA65_RS01675 at 364.699 kb on - strand, within DZA65_RS01675 at 364.723 kb on + strand, within DZA65_RS01675 at 364.866 kb on - strand at 364.896 kb on - strand at 364.896 kb on - strand at 365.005 kb on + strand at 365.005 kb on + strand at 365.006 kb on - strand at 365.006 kb on - strand at 365.079 kb on - strand, within DZA65_RS01680 at 365.132 kb on - strand, within DZA65_RS01680 at 365.132 kb on - strand, within DZA65_RS01680 at 365.150 kb on - strand, within DZA65_RS01680 at 365.231 kb on + strand, within DZA65_RS01680 at 365.232 kb on - strand, within DZA65_RS01680 at 365.232 kb on - strand, within DZA65_RS01680 at 365.232 kb on - strand, within DZA65_RS01680 at 365.232 kb on - strand, within DZA65_RS01680 at 365.232 kb on - strand, within DZA65_RS01680 at 365.233 kb on + strand, within DZA65_RS01680 at 365.233 kb on + strand, within DZA65_RS01680 at 365.233 kb on + strand, within DZA65_RS01680 at 365.233 kb on + strand, within DZA65_RS01680 at 365.234 kb on - strand, within DZA65_RS01680 at 365.234 kb on - strand, within DZA65_RS01680 at 365.234 kb on - strand at 365.234 kb on - strand, within DZA65_RS01680 at 365.234 kb on - strand, within DZA65_RS01680 at 365.234 kb on - strand, within DZA65_RS01680 at 365.267 kb on + strand, within DZA65_RS01680 at 365.267 kb on + strand, within DZA65_RS01680 at 365.267 kb on + strand, within DZA65_RS01680 at 365.267 kb on + strand, within DZA65_RS01680 at 365.268 kb on - strand, within DZA65_RS01680 at 365.268 kb on - strand, within DZA65_RS01680 at 365.268 kb on - strand, within DZA65_RS01680 at 365.268 kb on - strand, within DZA65_RS01680
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Atlantic remove 362,822 + DZA65_RS01660 0.80 -0.4 362,850 - DZA65_RS01660 0.83 -1.2 362,851 + DZA65_RS01660 0.83 -0.6 362,851 + DZA65_RS01660 0.83 +0.4 362,851 + DZA65_RS01660 0.83 +1.4 362,851 + DZA65_RS01660 0.83 -1.9 362,851 + DZA65_RS01660 0.83 +0.1 362,851 + DZA65_RS01660 0.83 +2.9 362,852 - DZA65_RS01660 0.83 +0.1 362,852 - DZA65_RS01660 0.83 +0.5 362,857 + DZA65_RS01660 0.83 +0.1 362,858 - DZA65_RS01660 0.83 -1.5 362,858 - DZA65_RS01660 0.83 -0.2 362,858 - DZA65_RS01660 0.83 +0.2 362,956 + +0.9 362,957 - -0.9 363,078 + -0.2 363,079 - -0.5 363,083 - -0.0 363,094 + -0.3 363,095 - -2.5 363,099 - +0.5 363,110 + -0.7 363,197 - +2.1 363,197 - -0.2 363,213 + -0.2 363,214 - -1.9 363,214 - +0.1 363,214 - +0.9 363,549 - DZA65_RS01665 0.59 +0.5 363,778 - +0.1 363,778 - +0.5 363,778 - -2.0 363,778 - -0.5 363,778 - -0.9 363,778 - -1.5 363,799 + -0.9 363,800 - -0.5 363,800 - +0.3 363,827 + -0.5 363,828 - -0.5 363,834 + +0.4 363,834 + +1.1 363,834 + -1.7 363,834 + -0.3 363,834 + +1.5 363,834 + +2.8 363,835 - -1.0 363,863 + DZA65_RS01670 0.11 -0.3 363,863 + DZA65_RS01670 0.11 +0.1 363,864 - DZA65_RS01670 0.11 -0.3 363,864 - DZA65_RS01670 0.11 -2.6 363,864 - DZA65_RS01670 0.11 -0.0 363,864 - DZA65_RS01670 0.11 -1.2 363,864 - DZA65_RS01670 0.11 -0.7 363,864 - DZA65_RS01670 0.11 +0.8 363,955 + DZA65_RS01670 0.27 -0.1 364,093 + DZA65_RS01670 0.50 -0.7 364,142 + DZA65_RS01670 0.58 +0.5 364,151 + DZA65_RS01670 0.60 +0.3 364,151 + DZA65_RS01670 0.60 +1.5 364,151 + DZA65_RS01670 0.60 -0.2 364,152 - DZA65_RS01670 0.60 +0.4 364,152 - DZA65_RS01670 0.60 +1.3 364,172 + DZA65_RS01670 0.63 -1.2 364,172 + DZA65_RS01670 0.63 -0.2 364,202 + DZA65_RS01670 0.68 -0.2 364,203 - DZA65_RS01670 0.69 -0.9 364,203 - DZA65_RS01670 0.69 +1.5 364,203 - DZA65_RS01670 0.69 +1.8 364,373 + -0.9 364,373 + -1.2 364,374 - -0.5 364,374 - +1.3 364,374 - +0.1 364,407 + -1.7 364,408 - -0.3 364,439 + +1.3 364,439 + +1.5 364,439 + +0.5 364,439 + -0.1 364,440 - -1.6 364,440 - +2.7 364,451 + -0.7 364,452 - +0.7 364,452 - -2.2 364,464 - -0.7 364,620 + DZA65_RS01675 0.40 -1.2 364,620 + DZA65_RS01675 0.40 -0.4 364,651 - DZA65_RS01675 0.48 -1.3 364,698 + DZA65_RS01675 0.60 -0.1 364,699 - DZA65_RS01675 0.60 +0.3 364,699 - DZA65_RS01675 0.60 -1.7 364,723 + DZA65_RS01675 0.66 +0.0 364,866 - -0.2 364,896 - +0.9 364,896 - +0.5 365,005 + +0.5 365,005 + +0.2 365,006 - -0.1 365,006 - -0.3 365,079 - DZA65_RS01680 0.13 -2.1 365,132 - DZA65_RS01680 0.15 -2.5 365,132 - DZA65_RS01680 0.15 +0.1 365,150 - DZA65_RS01680 0.16 -1.2 365,231 + DZA65_RS01680 0.20 -0.5 365,232 - DZA65_RS01680 0.20 +0.4 365,232 - DZA65_RS01680 0.20 -0.6 365,232 - DZA65_RS01680 0.20 -1.6 365,232 - DZA65_RS01680 0.20 -1.2 365,232 - DZA65_RS01680 0.20 +0.1 365,233 + DZA65_RS01680 0.20 -0.9 365,233 + DZA65_RS01680 0.20 +0.1 365,233 + DZA65_RS01680 0.20 -0.8 365,233 + DZA65_RS01680 0.20 -1.7 365,234 - DZA65_RS01680 0.20 -2.2 365,234 - DZA65_RS01680 0.20 -1.5 365,234 - -0.2 365,234 - DZA65_RS01680 0.20 +0.1 365,234 - DZA65_RS01680 0.20 -2.6 365,234 - DZA65_RS01680 0.20 -1.9 365,267 + DZA65_RS01680 0.22 +0.1 365,267 + DZA65_RS01680 0.22 -2.1 365,267 + DZA65_RS01680 0.22 +0.7 365,267 + DZA65_RS01680 0.22 +0.5 365,268 - DZA65_RS01680 0.22 +2.1 365,268 - DZA65_RS01680 0.22 -1.1 365,268 - DZA65_RS01680 0.22 -0.6 365,268 - DZA65_RS01680 0.22 -1.9
Or see this region's nucleotide sequence