Experiment: Stem-Atlantic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS10775 and DZA65_RS10780 are separated by 266 nucleotides DZA65_RS10780 and DZA65_RS10785 are separated by 84 nucleotides DZA65_RS10785 and DZA65_RS10790 are separated by 5 nucleotides DZA65_RS10790 and DZA65_RS10795 are separated by 45 nucleotides
DZA65_RS10775: DZA65_RS10775 - signal peptide peptidase SppA, at 2,279,493 to 2,281,343
_RS10775
DZA65_RS10780: DZA65_RS10780 - NAD(P)H nitroreductase, at 2,281,610 to 2,282,161
_RS10780
DZA65_RS10785: DZA65_RS10785 - DNA topoisomerase III, at 2,282,246 to 2,284,171
_RS10785
DZA65_RS10790: DZA65_RS10790 - DUF1496 domain-containing protein, at 2,284,177 to 2,284,572
_RS10790
DZA65_RS10795: DZA65_RS10795 - exodeoxyribonuclease III, at 2,284,618 to 2,285,424
_RS10795
Position (kb)
2282
2283
2284
2285 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2281.269 kb on - strand at 2281.312 kb on - strand at 2281.372 kb on + strand at 2281.417 kb on + strand at 2281.476 kb on - strand at 2281.476 kb on - strand at 2281.486 kb on + strand at 2281.486 kb on + strand at 2281.486 kb on + strand at 2281.486 kb on + strand at 2281.490 kb on + strand at 2281.490 kb on + strand at 2281.491 kb on - strand at 2281.491 kb on - strand at 2281.491 kb on - strand at 2281.491 kb on - strand at 2281.539 kb on + strand at 2281.540 kb on - strand at 2281.548 kb on + strand at 2281.571 kb on + strand at 2281.571 kb on + strand at 2281.571 kb on + strand at 2281.572 kb on - strand at 2281.572 kb on - strand at 2281.629 kb on + strand at 2281.693 kb on + strand, within DZA65_RS10780 at 2281.909 kb on + strand, within DZA65_RS10780 at 2281.909 kb on + strand, within DZA65_RS10780 at 2281.910 kb on - strand, within DZA65_RS10780 at 2281.935 kb on + strand, within DZA65_RS10780 at 2281.996 kb on + strand, within DZA65_RS10780 at 2282.085 kb on - strand, within DZA65_RS10780 at 2282.085 kb on - strand, within DZA65_RS10780 at 2282.085 kb on - strand, within DZA65_RS10780 at 2282.134 kb on + strand at 2282.135 kb on - strand at 2282.153 kb on + strand at 2282.153 kb on + strand at 2282.153 kb on + strand at 2282.153 kb on + strand at 2282.154 kb on - strand at 2282.154 kb on - strand at 2282.154 kb on - strand at 2282.159 kb on + strand at 2282.159 kb on + strand at 2282.160 kb on - strand at 2282.221 kb on + strand at 2282.405 kb on + strand at 2282.405 kb on + strand at 2282.405 kb on + strand at 2282.406 kb on - strand at 2282.574 kb on + strand, within DZA65_RS10785 at 2282.574 kb on + strand, within DZA65_RS10785 at 2282.598 kb on - strand, within DZA65_RS10785 at 2282.760 kb on - strand, within DZA65_RS10785 at 2282.760 kb on - strand, within DZA65_RS10785 at 2282.760 kb on - strand, within DZA65_RS10785 at 2282.783 kb on + strand, within DZA65_RS10785 at 2282.784 kb on - strand, within DZA65_RS10785 at 2282.784 kb on - strand, within DZA65_RS10785 at 2282.784 kb on - strand, within DZA65_RS10785 at 2282.784 kb on - strand, within DZA65_RS10785 at 2282.784 kb on - strand, within DZA65_RS10785 at 2282.784 kb on - strand, within DZA65_RS10785 at 2282.785 kb on + strand, within DZA65_RS10785 at 2282.785 kb on + strand, within DZA65_RS10785 at 2282.786 kb on - strand, within DZA65_RS10785 at 2282.786 kb on - strand, within DZA65_RS10785 at 2282.786 kb on - strand, within DZA65_RS10785 at 2282.786 kb on - strand, within DZA65_RS10785 at 2282.786 kb on - strand, within DZA65_RS10785 at 2282.786 kb on - strand, within DZA65_RS10785 at 2282.906 kb on + strand, within DZA65_RS10785 at 2282.907 kb on - strand, within DZA65_RS10785 at 2282.990 kb on + strand at 2283.080 kb on + strand, within DZA65_RS10785 at 2283.080 kb on + strand, within DZA65_RS10785 at 2283.081 kb on - strand, within DZA65_RS10785 at 2283.081 kb on - strand, within DZA65_RS10785 at 2283.081 kb on - strand, within DZA65_RS10785 at 2283.081 kb on - strand, within DZA65_RS10785 at 2283.081 kb on - strand, within DZA65_RS10785 at 2283.082 kb on + strand, within DZA65_RS10785 at 2283.082 kb on + strand, within DZA65_RS10785 at 2283.083 kb on - strand, within DZA65_RS10785 at 2283.083 kb on - strand, within DZA65_RS10785 at 2283.083 kb on - strand, within DZA65_RS10785 at 2283.088 kb on + strand, within DZA65_RS10785 at 2283.204 kb on - strand, within DZA65_RS10785 at 2283.223 kb on + strand, within DZA65_RS10785 at 2283.223 kb on + strand, within DZA65_RS10785 at 2283.223 kb on + strand, within DZA65_RS10785 at 2283.224 kb on - strand, within DZA65_RS10785 at 2283.224 kb on - strand, within DZA65_RS10785 at 2283.285 kb on + strand, within DZA65_RS10785 at 2283.397 kb on + strand, within DZA65_RS10785 at 2283.398 kb on - strand, within DZA65_RS10785 at 2283.422 kb on - strand, within DZA65_RS10785 at 2283.452 kb on + strand, within DZA65_RS10785 at 2283.452 kb on + strand, within DZA65_RS10785 at 2283.473 kb on + strand, within DZA65_RS10785 at 2283.474 kb on - strand, within DZA65_RS10785 at 2283.474 kb on - strand, within DZA65_RS10785 at 2283.474 kb on - strand, within DZA65_RS10785 at 2283.508 kb on + strand, within DZA65_RS10785 at 2283.509 kb on - strand, within DZA65_RS10785 at 2283.765 kb on + strand, within DZA65_RS10785 at 2283.766 kb on - strand at 2283.884 kb on + strand, within DZA65_RS10785 at 2283.884 kb on + strand, within DZA65_RS10785 at 2283.885 kb on - strand at 2283.886 kb on + strand, within DZA65_RS10785 at 2283.886 kb on + strand at 2283.886 kb on + strand, within DZA65_RS10785 at 2283.887 kb on - strand, within DZA65_RS10785 at 2283.887 kb on - strand, within DZA65_RS10785 at 2283.887 kb on - strand, within DZA65_RS10785 at 2283.887 kb on - strand, within DZA65_RS10785 at 2283.887 kb on - strand, within DZA65_RS10785 at 2284.051 kb on + strand at 2284.052 kb on - strand at 2284.075 kb on + strand at 2284.076 kb on - strand at 2284.078 kb on + strand at 2284.079 kb on - strand at 2284.220 kb on + strand, within DZA65_RS10790 at 2284.220 kb on + strand, within DZA65_RS10790 at 2284.260 kb on + strand, within DZA65_RS10790 at 2284.261 kb on - strand, within DZA65_RS10790 at 2284.281 kb on - strand, within DZA65_RS10790 at 2284.302 kb on + strand, within DZA65_RS10790 at 2284.374 kb on + strand, within DZA65_RS10790 at 2284.460 kb on - strand, within DZA65_RS10790 at 2284.460 kb on - strand, within DZA65_RS10790 at 2284.570 kb on + strand at 2284.577 kb on - strand at 2284.577 kb on - strand at 2284.577 kb on - strand at 2284.577 kb on - strand at 2284.683 kb on - strand at 2284.683 kb on - strand at 2284.732 kb on - strand, within DZA65_RS10795 at 2284.939 kb on - strand, within DZA65_RS10795 at 2284.939 kb on - strand, within DZA65_RS10795 at 2284.981 kb on - strand, within DZA65_RS10795 at 2285.021 kb on + strand, within DZA65_RS10795 at 2285.021 kb on + strand, within DZA65_RS10795 at 2285.021 kb on + strand, within DZA65_RS10795 at 2285.022 kb on - strand, within DZA65_RS10795 at 2285.022 kb on - strand, within DZA65_RS10795 at 2285.039 kb on + strand, within DZA65_RS10795 at 2285.040 kb on - strand, within DZA65_RS10795 at 2285.040 kb on - strand, within DZA65_RS10795 at 2285.040 kb on - strand, within DZA65_RS10795 at 2285.154 kb on + strand, within DZA65_RS10795
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Atlantic remove 2,281,269 - +0.5 2,281,312 - -1.6 2,281,372 + +1.2 2,281,417 + -0.1 2,281,476 - -0.5 2,281,476 - +0.8 2,281,486 + -0.5 2,281,486 + +0.1 2,281,486 + -0.9 2,281,486 + +0.5 2,281,490 + -0.5 2,281,490 + +0.5 2,281,491 - -0.1 2,281,491 - -0.2 2,281,491 - -0.7 2,281,491 - +2.5 2,281,539 + +0.8 2,281,540 - -0.1 2,281,548 + +0.3 2,281,571 + +0.5 2,281,571 + -0.4 2,281,571 + -0.2 2,281,572 - -1.8 2,281,572 - -0.0 2,281,629 + -0.4 2,281,693 + DZA65_RS10780 0.15 +1.5 2,281,909 + DZA65_RS10780 0.54 +0.1 2,281,909 + DZA65_RS10780 0.54 +0.6 2,281,910 - DZA65_RS10780 0.54 +0.1 2,281,935 + DZA65_RS10780 0.59 +0.2 2,281,996 + DZA65_RS10780 0.70 +1.1 2,282,085 - DZA65_RS10780 0.86 +0.5 2,282,085 - DZA65_RS10780 0.86 +0.4 2,282,085 - DZA65_RS10780 0.86 -0.2 2,282,134 + -0.5 2,282,135 - -0.5 2,282,153 + +0.1 2,282,153 + +0.1 2,282,153 + -1.1 2,282,153 + -0.8 2,282,154 - -0.2 2,282,154 - -1.3 2,282,154 - +0.2 2,282,159 + +1.0 2,282,159 + +0.5 2,282,160 - +0.5 2,282,221 + +0.1 2,282,405 + +2.5 2,282,405 + +2.3 2,282,405 + +1.5 2,282,406 - +0.5 2,282,574 + DZA65_RS10785 0.17 -0.4 2,282,574 + DZA65_RS10785 0.17 -1.3 2,282,598 - DZA65_RS10785 0.18 -0.5 2,282,760 - DZA65_RS10785 0.27 +2.9 2,282,760 - DZA65_RS10785 0.27 +1.1 2,282,760 - DZA65_RS10785 0.27 +0.5 2,282,783 + DZA65_RS10785 0.28 +0.2 2,282,784 - DZA65_RS10785 0.28 -2.9 2,282,784 - DZA65_RS10785 0.28 -1.3 2,282,784 - DZA65_RS10785 0.28 +0.5 2,282,784 - DZA65_RS10785 0.28 +0.3 2,282,784 - DZA65_RS10785 0.28 -0.5 2,282,784 - DZA65_RS10785 0.28 -0.2 2,282,785 + DZA65_RS10785 0.28 -0.1 2,282,785 + DZA65_RS10785 0.28 +2.1 2,282,786 - DZA65_RS10785 0.28 +0.1 2,282,786 - DZA65_RS10785 0.28 +0.4 2,282,786 - DZA65_RS10785 0.28 +1.0 2,282,786 - DZA65_RS10785 0.28 +0.3 2,282,786 - DZA65_RS10785 0.28 -1.6 2,282,786 - DZA65_RS10785 0.28 +1.0 2,282,906 + DZA65_RS10785 0.34 +1.5 2,282,907 - DZA65_RS10785 0.34 -2.3 2,282,990 + -2.3 2,283,080 + DZA65_RS10785 0.43 -1.0 2,283,080 + DZA65_RS10785 0.43 +0.5 2,283,081 - DZA65_RS10785 0.43 -0.2 2,283,081 - DZA65_RS10785 0.43 +0.7 2,283,081 - DZA65_RS10785 0.43 +0.2 2,283,081 - DZA65_RS10785 0.43 +1.3 2,283,081 - DZA65_RS10785 0.43 -0.3 2,283,082 + DZA65_RS10785 0.43 +0.2 2,283,082 + DZA65_RS10785 0.43 +0.7 2,283,083 - DZA65_RS10785 0.43 +0.2 2,283,083 - DZA65_RS10785 0.43 -0.0 2,283,083 - DZA65_RS10785 0.43 -2.0 2,283,088 + DZA65_RS10785 0.44 -0.2 2,283,204 - DZA65_RS10785 0.50 +0.8 2,283,223 + DZA65_RS10785 0.51 +0.8 2,283,223 + DZA65_RS10785 0.51 -1.9 2,283,223 + DZA65_RS10785 0.51 +0.5 2,283,224 - DZA65_RS10785 0.51 +0.5 2,283,224 - DZA65_RS10785 0.51 +1.9 2,283,285 + DZA65_RS10785 0.54 +0.4 2,283,397 + DZA65_RS10785 0.60 -0.5 2,283,398 - DZA65_RS10785 0.60 +0.5 2,283,422 - DZA65_RS10785 0.61 +2.9 2,283,452 + DZA65_RS10785 0.63 +1.2 2,283,452 + DZA65_RS10785 0.63 +1.5 2,283,473 + DZA65_RS10785 0.64 -0.1 2,283,474 - DZA65_RS10785 0.64 -0.6 2,283,474 - DZA65_RS10785 0.64 -2.3 2,283,474 - DZA65_RS10785 0.64 +1.1 2,283,508 + DZA65_RS10785 0.66 +1.3 2,283,509 - DZA65_RS10785 0.66 +0.8 2,283,765 + DZA65_RS10785 0.79 -0.0 2,283,766 - +0.3 2,283,884 + DZA65_RS10785 0.85 -0.3 2,283,884 + DZA65_RS10785 0.85 -0.5 2,283,885 - -1.5 2,283,886 + DZA65_RS10785 0.85 -0.8 2,283,886 + -0.0 2,283,886 + DZA65_RS10785 0.85 +0.1 2,283,887 - DZA65_RS10785 0.85 -0.0 2,283,887 - DZA65_RS10785 0.85 -0.5 2,283,887 - DZA65_RS10785 0.85 -0.3 2,283,887 - DZA65_RS10785 0.85 -0.6 2,283,887 - DZA65_RS10785 0.85 -0.5 2,284,051 + -0.9 2,284,052 - +1.9 2,284,075 + -3.0 2,284,076 - -0.3 2,284,078 + -0.1 2,284,079 - -0.5 2,284,220 + DZA65_RS10790 0.11 +1.1 2,284,220 + DZA65_RS10790 0.11 -0.0 2,284,260 + DZA65_RS10790 0.21 +1.9 2,284,261 - DZA65_RS10790 0.21 +0.5 2,284,281 - DZA65_RS10790 0.26 +1.1 2,284,302 + DZA65_RS10790 0.32 -0.5 2,284,374 + DZA65_RS10790 0.50 +1.0 2,284,460 - DZA65_RS10790 0.71 +0.5 2,284,460 - DZA65_RS10790 0.71 -0.8 2,284,570 + -1.5 2,284,577 - -1.1 2,284,577 - +1.4 2,284,577 - +0.3 2,284,577 - -0.7 2,284,683 - -1.3 2,284,683 - -2.5 2,284,732 - DZA65_RS10795 0.14 +1.5 2,284,939 - DZA65_RS10795 0.40 -0.6 2,284,939 - DZA65_RS10795 0.40 +0.3 2,284,981 - DZA65_RS10795 0.45 -0.3 2,285,021 + DZA65_RS10795 0.50 +1.4 2,285,021 + DZA65_RS10795 0.50 -1.0 2,285,021 + DZA65_RS10795 0.50 -1.8 2,285,022 - DZA65_RS10795 0.50 -0.8 2,285,022 - DZA65_RS10795 0.50 +2.1 2,285,039 + DZA65_RS10795 0.52 -0.7 2,285,040 - DZA65_RS10795 0.52 -1.0 2,285,040 - DZA65_RS10795 0.52 +0.7 2,285,040 - DZA65_RS10795 0.52 +1.0 2,285,154 + DZA65_RS10795 0.66 +0.1
Or see this region's nucleotide sequence