Experiment: Stem-Atlantic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS10510 and DZA65_RS10515 are separated by 40 nucleotides DZA65_RS10515 and DZA65_RS10520 are separated by 293 nucleotides DZA65_RS10520 and DZA65_RS10525 are separated by 206 nucleotides DZA65_RS10525 and DZA65_RS10530 are separated by 7 nucleotides
DZA65_RS10510: DZA65_RS10510 - YebC/PmpR family DNA-binding transcriptional regulator, at 2,229,591 to 2,230,334
_RS10510
DZA65_RS10515: DZA65_RS10515 - crossover junction endodeoxyribonuclease RuvC, at 2,230,375 to 2,230,896
_RS10515
DZA65_RS10520: DZA65_RS10520 - HoxN/HupN/NixA family nickel/cobalt transporter, at 2,231,190 to 2,232,221
_RS10520
DZA65_RS10525: DZA65_RS10525 - Holliday junction branch migration protein RuvA, at 2,232,428 to 2,233,042
_RS10525
DZA65_RS10530: DZA65_RS10530 - Holliday junction branch migration DNA helicase RuvB, at 2,233,050 to 2,234,054
_RS10530
Position (kb)
2231
2232
2233 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2230.250 kb on + strand, within DZA65_RS10510 at 2230.256 kb on + strand, within DZA65_RS10510 at 2230.318 kb on + strand at 2230.380 kb on + strand at 2230.380 kb on + strand at 2230.380 kb on + strand at 2230.383 kb on + strand at 2230.888 kb on - strand at 2230.952 kb on + strand at 2230.952 kb on + strand at 2230.952 kb on + strand at 2230.952 kb on + strand at 2230.953 kb on - strand at 2231.060 kb on + strand at 2231.060 kb on + strand at 2231.061 kb on - strand at 2231.061 kb on - strand at 2231.061 kb on - strand at 2231.073 kb on + strand at 2231.074 kb on - strand at 2231.086 kb on + strand at 2231.087 kb on - strand at 2231.113 kb on - strand at 2231.113 kb on - strand at 2231.113 kb on - strand at 2231.113 kb on - strand at 2231.124 kb on - strand at 2231.176 kb on - strand at 2231.176 kb on - strand at 2231.183 kb on + strand at 2231.213 kb on + strand at 2231.213 kb on + strand at 2231.214 kb on - strand at 2231.233 kb on + strand at 2231.256 kb on - strand at 2231.260 kb on + strand at 2231.260 kb on + strand at 2231.261 kb on - strand at 2231.261 kb on - strand at 2231.323 kb on + strand, within DZA65_RS10520 at 2231.324 kb on - strand, within DZA65_RS10520 at 2231.338 kb on - strand, within DZA65_RS10520 at 2231.369 kb on + strand, within DZA65_RS10520 at 2231.509 kb on + strand, within DZA65_RS10520 at 2231.509 kb on + strand, within DZA65_RS10520 at 2231.509 kb on + strand, within DZA65_RS10520 at 2231.510 kb on - strand, within DZA65_RS10520 at 2231.510 kb on - strand, within DZA65_RS10520 at 2231.519 kb on + strand, within DZA65_RS10520 at 2231.642 kb on + strand, within DZA65_RS10520 at 2231.720 kb on + strand, within DZA65_RS10520 at 2231.720 kb on + strand, within DZA65_RS10520 at 2231.721 kb on - strand, within DZA65_RS10520 at 2231.721 kb on - strand, within DZA65_RS10520 at 2231.754 kb on + strand, within DZA65_RS10520 at 2231.754 kb on + strand, within DZA65_RS10520 at 2231.754 kb on + strand, within DZA65_RS10520 at 2231.754 kb on + strand, within DZA65_RS10520 at 2231.754 kb on + strand, within DZA65_RS10520 at 2231.754 kb on + strand, within DZA65_RS10520 at 2231.754 kb on + strand, within DZA65_RS10520 at 2231.754 kb on + strand, within DZA65_RS10520 at 2231.755 kb on - strand, within DZA65_RS10520 at 2231.755 kb on - strand, within DZA65_RS10520 at 2231.755 kb on - strand, within DZA65_RS10520 at 2231.755 kb on - strand, within DZA65_RS10520 at 2231.755 kb on - strand, within DZA65_RS10520 at 2231.755 kb on - strand, within DZA65_RS10520 at 2231.755 kb on - strand, within DZA65_RS10520 at 2231.755 kb on - strand, within DZA65_RS10520 at 2231.755 kb on - strand, within DZA65_RS10520 at 2231.755 kb on - strand, within DZA65_RS10520 at 2231.755 kb on - strand, within DZA65_RS10520 at 2231.755 kb on - strand, within DZA65_RS10520 at 2231.810 kb on - strand, within DZA65_RS10520 at 2231.849 kb on + strand, within DZA65_RS10520 at 2231.849 kb on + strand, within DZA65_RS10520 at 2231.849 kb on + strand, within DZA65_RS10520 at 2231.850 kb on - strand, within DZA65_RS10520 at 2231.882 kb on + strand, within DZA65_RS10520 at 2231.915 kb on + strand, within DZA65_RS10520 at 2231.916 kb on - strand at 2231.937 kb on + strand, within DZA65_RS10520 at 2231.938 kb on - strand, within DZA65_RS10520 at 2231.974 kb on - strand, within DZA65_RS10520 at 2231.974 kb on - strand, within DZA65_RS10520 at 2231.976 kb on + strand, within DZA65_RS10520 at 2231.976 kb on + strand, within DZA65_RS10520 at 2231.976 kb on + strand, within DZA65_RS10520 at 2231.976 kb on + strand, within DZA65_RS10520 at 2231.976 kb on + strand, within DZA65_RS10520 at 2231.976 kb on + strand, within DZA65_RS10520 at 2231.976 kb on + strand, within DZA65_RS10520 at 2231.976 kb on + strand, within DZA65_RS10520 at 2231.976 kb on + strand, within DZA65_RS10520 at 2231.976 kb on + strand, within DZA65_RS10520 at 2231.976 kb on + strand, within DZA65_RS10520 at 2231.977 kb on - strand at 2231.977 kb on - strand, within DZA65_RS10520 at 2231.977 kb on - strand, within DZA65_RS10520 at 2231.977 kb on - strand, within DZA65_RS10520 at 2232.056 kb on + strand, within DZA65_RS10520 at 2232.129 kb on + strand at 2232.129 kb on + strand at 2232.130 kb on - strand at 2232.130 kb on - strand at 2232.162 kb on - strand at 2232.178 kb on - strand at 2232.193 kb on - strand at 2232.206 kb on + strand at 2232.206 kb on + strand at 2232.220 kb on - strand at 2232.330 kb on - strand at 2232.330 kb on - strand at 2232.330 kb on - strand at 2232.331 kb on + strand at 2232.332 kb on - strand at 2232.379 kb on + strand at 2232.379 kb on + strand at 2232.380 kb on - strand at 2232.380 kb on - strand at 2232.380 kb on - strand at 2232.380 kb on - strand at 2232.381 kb on + strand at 2232.381 kb on + strand at 2232.382 kb on - strand at 2232.382 kb on - strand at 2232.382 kb on - strand at 2232.386 kb on + strand at 2232.386 kb on + strand at 2232.386 kb on + strand at 2232.386 kb on + strand at 2232.386 kb on + strand at 2232.387 kb on - strand at 2232.514 kb on + strand, within DZA65_RS10525 at 2233.045 kb on + strand at 2233.045 kb on + strand at 2233.045 kb on + strand at 2233.045 kb on + strand at 2233.045 kb on + strand at 2233.045 kb on + strand at 2233.049 kb on + strand at 2233.049 kb on + strand at 2233.049 kb on + strand at 2233.049 kb on + strand at 2233.049 kb on + strand at 2233.049 kb on + strand at 2233.049 kb on + strand at 2233.049 kb on + strand at 2233.049 kb on + strand at 2233.049 kb on + strand at 2233.049 kb on + strand at 2233.049 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Atlantic remove 2,230,250 + DZA65_RS10510 0.89 -0.5 2,230,256 + DZA65_RS10510 0.89 +0.8 2,230,318 + -0.7 2,230,380 + +0.5 2,230,380 + +0.6 2,230,380 + -1.0 2,230,383 + +0.3 2,230,888 - -0.0 2,230,952 + -1.8 2,230,952 + -0.0 2,230,952 + -0.2 2,230,952 + -0.4 2,230,953 - +0.3 2,231,060 + -0.2 2,231,060 + +1.5 2,231,061 - +1.1 2,231,061 - -0.6 2,231,061 - -0.5 2,231,073 + -0.8 2,231,074 - +2.9 2,231,086 + -1.5 2,231,087 - -3.0 2,231,113 - +0.2 2,231,113 - -0.8 2,231,113 - +0.5 2,231,113 - +0.1 2,231,124 - +1.9 2,231,176 - +0.5 2,231,176 - +0.1 2,231,183 + +0.1 2,231,213 + +0.5 2,231,213 + +0.1 2,231,214 - -0.5 2,231,233 + +0.5 2,231,256 - -1.2 2,231,260 + -0.1 2,231,260 + -1.8 2,231,261 - +1.3 2,231,261 - +0.2 2,231,323 + DZA65_RS10520 0.13 -0.0 2,231,324 - DZA65_RS10520 0.13 +0.1 2,231,338 - DZA65_RS10520 0.14 +1.0 2,231,369 + DZA65_RS10520 0.17 +0.3 2,231,509 + DZA65_RS10520 0.31 -0.5 2,231,509 + DZA65_RS10520 0.31 -0.5 2,231,509 + DZA65_RS10520 0.31 +0.8 2,231,510 - DZA65_RS10520 0.31 -1.3 2,231,510 - DZA65_RS10520 0.31 -1.3 2,231,519 + DZA65_RS10520 0.32 -0.7 2,231,642 + DZA65_RS10520 0.44 -0.6 2,231,720 + DZA65_RS10520 0.51 -0.6 2,231,720 + DZA65_RS10520 0.51 -0.3 2,231,721 - DZA65_RS10520 0.51 -1.5 2,231,721 - DZA65_RS10520 0.51 -0.0 2,231,754 + DZA65_RS10520 0.55 -0.1 2,231,754 + DZA65_RS10520 0.55 +1.8 2,231,754 + DZA65_RS10520 0.55 -0.4 2,231,754 + DZA65_RS10520 0.55 +0.1 2,231,754 + DZA65_RS10520 0.55 -0.5 2,231,754 + DZA65_RS10520 0.55 +0.2 2,231,754 + DZA65_RS10520 0.55 -0.7 2,231,754 + DZA65_RS10520 0.55 +0.2 2,231,755 - DZA65_RS10520 0.55 -1.0 2,231,755 - DZA65_RS10520 0.55 +0.5 2,231,755 - DZA65_RS10520 0.55 -1.2 2,231,755 - DZA65_RS10520 0.55 -0.5 2,231,755 - DZA65_RS10520 0.55 +0.7 2,231,755 - DZA65_RS10520 0.55 +0.1 2,231,755 - DZA65_RS10520 0.55 -0.5 2,231,755 - DZA65_RS10520 0.55 -0.4 2,231,755 - DZA65_RS10520 0.55 -0.3 2,231,755 - DZA65_RS10520 0.55 +0.7 2,231,755 - DZA65_RS10520 0.55 -1.5 2,231,755 - DZA65_RS10520 0.55 +0.5 2,231,810 - DZA65_RS10520 0.60 +0.1 2,231,849 + DZA65_RS10520 0.64 -0.9 2,231,849 + DZA65_RS10520 0.64 -0.0 2,231,849 + DZA65_RS10520 0.64 +1.1 2,231,850 - DZA65_RS10520 0.64 +0.2 2,231,882 + DZA65_RS10520 0.67 +0.9 2,231,915 + DZA65_RS10520 0.70 +0.4 2,231,916 - -1.8 2,231,937 + DZA65_RS10520 0.72 -0.2 2,231,938 - DZA65_RS10520 0.72 +0.5 2,231,974 - DZA65_RS10520 0.76 +0.3 2,231,974 - DZA65_RS10520 0.76 +2.1 2,231,976 + DZA65_RS10520 0.76 +0.6 2,231,976 + DZA65_RS10520 0.76 +0.2 2,231,976 + DZA65_RS10520 0.76 -0.0 2,231,976 + DZA65_RS10520 0.76 +0.0 2,231,976 + DZA65_RS10520 0.76 -0.2 2,231,976 + DZA65_RS10520 0.76 +1.1 2,231,976 + DZA65_RS10520 0.76 -0.3 2,231,976 + DZA65_RS10520 0.76 -0.8 2,231,976 + DZA65_RS10520 0.76 +0.5 2,231,976 + DZA65_RS10520 0.76 +0.5 2,231,976 + DZA65_RS10520 0.76 -0.1 2,231,977 - -0.2 2,231,977 - DZA65_RS10520 0.76 -0.1 2,231,977 - DZA65_RS10520 0.76 -0.5 2,231,977 - DZA65_RS10520 0.76 +1.1 2,232,056 + DZA65_RS10520 0.84 +1.0 2,232,129 + +0.5 2,232,129 + +0.5 2,232,130 - -0.1 2,232,130 - -0.7 2,232,162 - +0.5 2,232,178 - +1.5 2,232,193 - +1.0 2,232,206 + -0.8 2,232,206 + +1.7 2,232,220 - +0.3 2,232,330 - +0.1 2,232,330 - -0.7 2,232,330 - +1.0 2,232,331 + +1.0 2,232,332 - -0.3 2,232,379 + -0.5 2,232,379 + -1.0 2,232,380 - -0.2 2,232,380 - +0.5 2,232,380 - -0.1 2,232,380 - +0.5 2,232,381 + +0.1 2,232,381 + +0.4 2,232,382 - -0.5 2,232,382 - -2.9 2,232,382 - -1.2 2,232,386 + -0.7 2,232,386 + -1.3 2,232,386 + -1.5 2,232,386 + -1.2 2,232,386 + +0.2 2,232,387 - -0.8 2,232,514 + DZA65_RS10525 0.14 -0.5 2,233,045 + -1.1 2,233,045 + -0.7 2,233,045 + -0.5 2,233,045 + -0.0 2,233,045 + +1.5 2,233,045 + -1.0 2,233,049 + -0.9 2,233,049 + -1.5 2,233,049 + +0.3 2,233,049 + +0.5 2,233,049 + +0.1 2,233,049 + -1.4 2,233,049 + -1.5 2,233,049 + -1.1 2,233,049 + -0.2 2,233,049 + +0.0 2,233,049 + -1.8 2,233,049 + +0.5
Or see this region's nucleotide sequence