Experiment: Stem-Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS05495 and DZA65_RS05500 are separated by 138 nucleotides DZA65_RS05500 and DZA65_RS05505 are separated by 3 nucleotides DZA65_RS05505 and DZA65_RS05510 are separated by 148 nucleotides DZA65_RS05510 and DZA65_RS05515 are separated by 3 nucleotides
DZA65_RS05495: DZA65_RS05495 - outer membrane protein assembly factor BamA, at 1,130,224 to 1,132,641
_RS05495
DZA65_RS05500: DZA65_RS05500 - molecular chaperone Skp, at 1,132,780 to 1,133,277
_RS05500
DZA65_RS05505: DZA65_RS05505 - UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase, at 1,133,281 to 1,134,303
_RS05505
DZA65_RS05510: DZA65_RS05510 - 3-hydroxyacyl-ACP dehydratase FabZ, at 1,134,452 to 1,134,907
_RS05510
DZA65_RS05515: DZA65_RS05515 - acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase, at 1,134,911 to 1,135,699
_RS05515
Position (kb)
1133
1134
1135 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1132.639 kb on + strand at 1132.639 kb on + strand at 1132.643 kb on + strand at 1132.643 kb on + strand at 1132.643 kb on + strand at 1132.643 kb on + strand at 1132.643 kb on + strand at 1132.643 kb on + strand at 1132.677 kb on + strand at 1132.677 kb on + strand at 1132.677 kb on + strand at 1132.677 kb on + strand at 1132.731 kb on + strand at 1132.757 kb on + strand at 1132.757 kb on + strand at 1132.757 kb on + strand at 1132.761 kb on + strand at 1132.761 kb on + strand at 1132.761 kb on + strand at 1132.808 kb on + strand at 1132.962 kb on + strand, within DZA65_RS05500 at 1132.962 kb on + strand, within DZA65_RS05500 at 1133.013 kb on + strand, within DZA65_RS05500 at 1133.013 kb on + strand, within DZA65_RS05500 at 1133.013 kb on + strand, within DZA65_RS05500 at 1133.224 kb on + strand, within DZA65_RS05500 at 1133.224 kb on + strand, within DZA65_RS05500 at 1133.224 kb on + strand, within DZA65_RS05500 at 1134.285 kb on + strand at 1134.336 kb on + strand at 1134.336 kb on + strand at 1134.336 kb on + strand at 1134.336 kb on + strand at 1134.336 kb on + strand at 1134.378 kb on + strand at 1134.378 kb on + strand at 1134.378 kb on + strand at 1134.378 kb on + strand at 1134.378 kb on + strand at 1134.415 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Upstate remove 1,132,639 + +0.8 1,132,639 + -0.9 1,132,643 + +0.9 1,132,643 + +0.1 1,132,643 + -2.2 1,132,643 + +1.2 1,132,643 + -1.7 1,132,643 + -2.3 1,132,677 + -2.8 1,132,677 + -0.3 1,132,677 + +0.7 1,132,677 + +1.2 1,132,731 + -2.2 1,132,757 + -2.4 1,132,757 + -1.4 1,132,757 + +0.6 1,132,761 + -0.1 1,132,761 + +0.9 1,132,761 + +1.2 1,132,808 + -0.3 1,132,962 + DZA65_RS05500 0.37 -0.7 1,132,962 + DZA65_RS05500 0.37 -3.8 1,133,013 + DZA65_RS05500 0.47 -1.4 1,133,013 + DZA65_RS05500 0.47 -1.2 1,133,013 + DZA65_RS05500 0.47 -0.8 1,133,224 + DZA65_RS05500 0.89 -1.9 1,133,224 + DZA65_RS05500 0.89 +0.2 1,133,224 + DZA65_RS05500 0.89 -0.1 1,134,285 + +0.2 1,134,336 + -0.2 1,134,336 + +2.2 1,134,336 + -3.1 1,134,336 + +2.7 1,134,336 + -0.1 1,134,378 + +0.5 1,134,378 + +1.5 1,134,378 + -0.5 1,134,378 + -0.1 1,134,378 + -1.7 1,134,415 + +0.9
Or see this region's nucleotide sequence